Figure 1 Baihui (GV20) and Fengchi (GB20) target information and intersection of acupoints with migraine targets A: the Venn diagram of Fengchi (GB20) and Baihui (GV20); B: the intersection of acupoints and migraine targets, in which red is the acupoint, gray and light green are the targets, and dark green are the intersecting targets; C: the target information of Fengchi (GB20) and Baihui (GV20).

Figure 2 Behavioral scores and changes in inflammatory factor levels in rats A: behavioral score; B: levels of IL-1β; C: levels of IL-6; D: levels of TNF-α, respectively, as measured via ELISA. After 30 min of successful modeling, the rats were immobilized. In the acupuncture group, "Baihui (GV20)" and "Fengchi (GB20)" were selected for acupuncture. The depth of needle insertion was approximately 2 mm, and the needle was retained for 30 min. The needles were manually twisted for 1 min with flat tonic and flat diarrhea, twisting once every 5 min for 1 min each time for 30 min. The electroacupuncture group was treated with a parse and dense waveform, frequency 2/15 Hz, and electroacupuncture intensity 1 mA, which lasted for 30 min. The model group and the control group only received routine immobilization for 0.5 h without any acupuncture intervention. IL-1β: interleukin-1 beta; IL-6: interleukin-6; TNF-α: tumor necrosis factor-alpha; ELISA: enzyme linked immunosorbent assay. Comparisons between groups were analyzed by Student's t tests, and the values are shown as the mean ± standard deviation (n = 10). Compared with the control group, aP < 0.05; compared with the Model group, bP < 0.05.

Figure 3 Effect of acupuncture on target proteins HMOX1, AKT1, STAT3, P-ERK1/ERK2 and TLR4 A: effects of acupuncture on target proteins HMOX1, AKT1 and STAT3; B: effects of acupuncture on target proteins P-ERK1/ERK2 and TLR4. After 30 min of successful modeling, the rats were immobilized. In the acupuncture group, "Baihui (GV20)" and "Fengchi (GB20)" were selected for acupuncture. The depth of needle insertion was approximately 2 mm, and the needle was retained for 30 min. The needles were manually twisted for 1 min with flat tonic and flat diarrhea, twisting once every 5 min for 1 min each time for 30 min. The electroacupuncture group was treated with a parse and dense waveform, frequency 2/15 Hz, and electroacupuncture intensity 1 mA, which lasted for 30 min. The model group and the control group only received routine immobilization for 0.5 h without any acupuncture intervention. All increased after modeling after acupuncture, twist or electroacupuncture intervention, the expression of the above proteins was reduced. HMOX1: heme oxygenase 1; AKT1: protein kinase B; STAT3: signal transducer and activator of transcription 3; P-ERK1/ERK2: extracellular signal-regulated kinase 1/extracellular signal-regulated kinase 2; TLR4: toll-like receptor 4; GAPDH: glyceraldehyde-3-phosphate dehydrogenase. Comparisons between groups were analyzed by Student's t tests, and the values are shown as the mean ± standard deviation (n = 10). Differences were considered statistically significant at P < 0.05.