Journal of Traditional Chinese Medicine ›› 2025, Vol. 45 ›› Issue (1): 13-21.DOI: 10.19852/j.cnki.jtcm.2025.01.002
Previous Articles Next Articles
Electroacupuncture enhances the mitophagy of granulosa cells in premature ovarian insufficiency model mice by inactivating the hippo-yes-associated protein/transcriptional co-activator with postsynaptic density protein, drosophila disc large tumor suppressor, and zonula occludens-1 protein binding motif pathway
WU Jiaman1,2(
), TANG Meng2, LUO Yu2, ZHU Haimin3, ZHAO Tianqi3, MA Fei1,2, NING Yan1,2()
- 1 Department of Chinese Medicine, Affiliated Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen 518028, China
2 Guangzhou University of Chinese Medicine, Guangzhou 510405, China
3 Southern Medical University, Guangzhou 510515, China
-
Received:2023-11-22Accepted:2024-04-25Online:2025-02-15Published:2025-01-10 -
Contact:WU Jiaman, Department of Chinese Medicine, Affiliated Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, China; Guangzhou University of Chinese Medicine, Guangzhou, China.DrWuJiaman@163.com ; NING Yan, Department of Chinese Medicine, Affiliated Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen 518028, China; Guangzhou University of Chinese Medicine, Guangzhou, China.Judyning@163.com , Telephone: +86-15817470407 -
Supported by:Shenzhen Science and Innovation Commission: Investigating the Mechanism of Action of Acupuncture in Regulating the Gut Microbiome to Inhibit Apoptosis of Ovarian Granulosa Cells in Premature Ovarian Insufficiency Mice based on the Rictor/Torepamycin Target Protein c2 Pathway(JCYJ20210324130001004);Sanming Project of Medicine in Shenzhen: the First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Luo Songping National Famous Chinese Medicine Practitioner Female Reproductive Disorders Prevention and Treatment Team(SZZYSM202311010)
Cite this article
WU Jiaman, TANG Meng, LUO Yu, ZHU Haimin, ZHAO Tianqi, MA Fei, NING Yan. Electroacupuncture enhances the mitophagy of granulosa cells in premature ovarian insufficiency model mice by inactivating the hippo-yes-associated protein/transcriptional co-activator with postsynaptic density protein, drosophila disc large tumor suppressor, and zonula occludens-1 protein binding motif pathway[J]. Journal of Traditional Chinese Medicine, 2025, 45(1): 13-21.
share this article
Figure 1 EA affects apoptosis of ovarian granulosa cells A: the TUNEL fluorescence staining assay was used to analyze apoptosis of granulosa cells. A1: DAPI stained ovarian granulosa cells in control group; A2: DAPI stained ovarian granulosa cells in POI group; A3: DAPI stained ovarian granulosa cells in POI + EA group; A4: TUNEL stained ovarian granulosa cells in control group; A5: TUNEL stained ovarian granulosa cells in POI group; A6: TUNEL stained ovarian granulosa cells in POI + EA group; A7: merge of DAPI and TUNEL stained ovarian granulosa cells in control group; A8: merge of DAPI and TUNEL stained ovarian granulosa cells in POI group; A9: merge of DAPI and TUNEL stained ovarian granulosa cells in POI + EA group. B: quantitative analysis of the TUNEL stained positive granulosa cells. Ctrl group: injected with normal saline. POI group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide. POI + EA group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide and the disposable sterile acupuncture needles were inserted into the Guanyuan (CV4) acupoint to a depth of 2 mm. The acupuncture needles were retained for 30 min per day for three weeks. EA: electroacupuncture; TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling; DAPI: 4',6-diamidino-2-phenylindole; Ctrl: control; POI: premature ovarian insufficiency. Statistical analyses were measured using one-way analysis of variance followed by Tukey's post hoc test. Data were presented as mean±standard deviation (n = 6). Compared with the control group, aP < 0.001; compared with the POI group, bP < 0.01.
Figure 1 EA affects apoptosis of ovarian granulosa cells A: the TUNEL fluorescence staining assay was used to analyze apoptosis of granulosa cells. A1: DAPI stained ovarian granulosa cells in control group; A2: DAPI stained ovarian granulosa cells in POI group; A3: DAPI stained ovarian granulosa cells in POI + EA group; A4: TUNEL stained ovarian granulosa cells in control group; A5: TUNEL stained ovarian granulosa cells in POI group; A6: TUNEL stained ovarian granulosa cells in POI + EA group; A7: merge of DAPI and TUNEL stained ovarian granulosa cells in control group; A8: merge of DAPI and TUNEL stained ovarian granulosa cells in POI group; A9: merge of DAPI and TUNEL stained ovarian granulosa cells in POI + EA group. B: quantitative analysis of the TUNEL stained positive granulosa cells. Ctrl group: injected with normal saline. POI group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide. POI + EA group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide and the disposable sterile acupuncture needles were inserted into the Guanyuan (CV4) acupoint to a depth of 2 mm. The acupuncture needles were retained for 30 min per day for three weeks. EA: electroacupuncture; TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling; DAPI: 4',6-diamidino-2-phenylindole; Ctrl: control; POI: premature ovarian insufficiency. Statistical analyses were measured using one-way analysis of variance followed by Tukey's post hoc test. Data were presented as mean±standard deviation (n = 6). Compared with the control group, aP < 0.001; compared with the POI group, bP < 0.01.
Figure 2 EA affects mitochondrial morphology and mitophagy of ovarian granulosa cells A: the mitochondrial morphology was observed under a transmission electron microscope. A1: control group; A2: POI group; A3: POI + EA group; B: the Western blotting assay was performed to access protein expression related to mitophagy; C: quantitative analysis of Pink1 protein levels. D: quantitative analysis of Parkin protein levels; E: quantitative analysis of LC3Ⅱ/Ⅰ protein levels. Control group: injected with normal saline. POI group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide. POI + EA group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide and the disposable sterile acupuncture needles were inserted into the Guanyuan (CV4) acupoint to a depth of 2 mm. The acupuncture needles were retained for 30 min per day for three weeks. Ctrl: control; EA: electroacupuncture; POI: premature ovarian insufficiency; LC3: microtubule-associated protein 1 light chain 3. Statistical analyses were measured using one-way analysis of variance followed by Tukey's post hoc test. Data were presented as mean ± standard deviation (n = 6). Compared with the control group, aP < 0.001; compared with the POI group, bP < 0.01.
Figure 2 EA affects mitochondrial morphology and mitophagy of ovarian granulosa cells A: the mitochondrial morphology was observed under a transmission electron microscope. A1: control group; A2: POI group; A3: POI + EA group; B: the Western blotting assay was performed to access protein expression related to mitophagy; C: quantitative analysis of Pink1 protein levels. D: quantitative analysis of Parkin protein levels; E: quantitative analysis of LC3Ⅱ/Ⅰ protein levels. Control group: injected with normal saline. POI group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide. POI + EA group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide and the disposable sterile acupuncture needles were inserted into the Guanyuan (CV4) acupoint to a depth of 2 mm. The acupuncture needles were retained for 30 min per day for three weeks. Ctrl: control; EA: electroacupuncture; POI: premature ovarian insufficiency; LC3: microtubule-associated protein 1 light chain 3. Statistical analyses were measured using one-way analysis of variance followed by Tukey's post hoc test. Data were presented as mean ± standard deviation (n = 6). Compared with the control group, aP < 0.001; compared with the POI group, bP < 0.01.
Figure 3 Representative photos of the key protein of JAK2/STAT3, Hippo-YAP/TAZ, and JNK/MAPK pathways were obtained by Western blotting assay A: representative protein bands of key protein of JAK2/STAT3 pathways in granulose cells; B: quantitative analysis of protein levels of p-JAK2/JAK2; C: quantitative analysis of protein levels of p-STAT3/STAT3; D: representative protein bands of key protein of Hippo-YAP/TAZ pathway in granulose cells; E: quantitative analysis of protein levels of p-YAP/YAP; F: quantitative analysis of protein levels of TAZ; G: quantitative analysis of protein levels of TEAD1; H: quantitative analysis of protein levels of MST1; I: representative protein bands of key protein of JNK/MAPK pathway in granulose cells; J: quantitative analysis of protein levels of p-JNK/JNK; K: quantitative analysis of protein levels of p-MAPK/MAPK. Control group: injected with normal saline. POI group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide. POI + EA group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide and the disposable sterile acupuncture needles were inserted into the Guanyuan (CV4) acupoint to a depth of 2 mm. The acupuncture needles were retained for 30 min per day for three weeks. EA: electroacupuncture; POI: premature ovarian insufficiency; JAK2: janus kinase 2; STAT3: signal transducer and activator of transcription 3; YAP1: yes-associated protein 1; TAZ: transcriptional co-activator with PDZ-binding motif; TEAD1: T-cell factor/enhancer of split and activator of transcription domain family member 1; MST1: mammalian sterile twenty-like kinase 1; JNK: c-jun n-terminal kinase 1; MAPK: mitogen-activated protein kinase. Statistical analyses were measured using one-way analysis of variance followed by Tukey's post hoc test. Data were presented as mean ± standard deviation (n = 6). Compared with the control group, aP < 0.001; compared with the POI group, bP < 0.01.
Figure 3 Representative photos of the key protein of JAK2/STAT3, Hippo-YAP/TAZ, and JNK/MAPK pathways were obtained by Western blotting assay A: representative protein bands of key protein of JAK2/STAT3 pathways in granulose cells; B: quantitative analysis of protein levels of p-JAK2/JAK2; C: quantitative analysis of protein levels of p-STAT3/STAT3; D: representative protein bands of key protein of Hippo-YAP/TAZ pathway in granulose cells; E: quantitative analysis of protein levels of p-YAP/YAP; F: quantitative analysis of protein levels of TAZ; G: quantitative analysis of protein levels of TEAD1; H: quantitative analysis of protein levels of MST1; I: representative protein bands of key protein of JNK/MAPK pathway in granulose cells; J: quantitative analysis of protein levels of p-JNK/JNK; K: quantitative analysis of protein levels of p-MAPK/MAPK. Control group: injected with normal saline. POI group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide. POI + EA group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide and the disposable sterile acupuncture needles were inserted into the Guanyuan (CV4) acupoint to a depth of 2 mm. The acupuncture needles were retained for 30 min per day for three weeks. EA: electroacupuncture; POI: premature ovarian insufficiency; JAK2: janus kinase 2; STAT3: signal transducer and activator of transcription 3; YAP1: yes-associated protein 1; TAZ: transcriptional co-activator with PDZ-binding motif; TEAD1: T-cell factor/enhancer of split and activator of transcription domain family member 1; MST1: mammalian sterile twenty-like kinase 1; JNK: c-jun n-terminal kinase 1; MAPK: mitogen-activated protein kinase. Statistical analyses were measured using one-way analysis of variance followed by Tukey's post hoc test. Data were presented as mean ± standard deviation (n = 6). Compared with the control group, aP < 0.001; compared with the POI group, bP < 0.01.
Table 1 Comparisons of the FSH, LH, E2, and AMH in the serum sample ($\bar{x}±s$)
| Group | n | FSH (U/L) | LH (ng/mL) | E2 (ng/L) | AMH (ng/L) |
|---|---|---|---|---|---|
| Ctrl | 6 | 2.79±0.34 | 2.12±0.24 | 23.00±1.50 | 67.46±5.57 |
| POI | 6 | 4.24±0.16a | 2.74±0.30a | 14.10±1.74a | 52.61±4.16a |
| POI + EA | 6 | 3.12±0.26b | 1.76±0.14b | 21.85±2.20b | 66.50±3.46b |
| POI + EA + VP | 6 | 3.74±0.40c | 2.53±0.16c | 16.37±1.31c | 55.74±4.31c |
Table 1 Comparisons of the FSH, LH, E2, and AMH in the serum sample ($\bar{x}±s$)
| Group | n | FSH (U/L) | LH (ng/mL) | E2 (ng/L) | AMH (ng/L) |
|---|---|---|---|---|---|
| Ctrl | 6 | 2.79±0.34 | 2.12±0.24 | 23.00±1.50 | 67.46±5.57 |
| POI | 6 | 4.24±0.16a | 2.74±0.30a | 14.10±1.74a | 52.61±4.16a |
| POI + EA | 6 | 3.12±0.26b | 1.76±0.14b | 21.85±2.20b | 66.50±3.46b |
| POI + EA + VP | 6 | 3.74±0.40c | 2.53±0.16c | 16.37±1.31c | 55.74±4.31c |
Figure 4 Verteporfin affects apoptosis, mitochondrial morphology and mitophagy of ovarian granulosa cells A: the TUNEL fluorescence staining assay was used to analyze apoptosis of granulosa cells. A1: DAPI stained ovarian granulosa cells in control group; A2: DAPI stained ovarian granulosa cells in POI group; A3: DAPI stained ovarian granulosa cells in POI + EA group; A4: DAPI stained ovarian granulosa cells in POI + EA + VP group; A5: TUNEL stained ovarian granulosa cells in control group; A6: TUNEL stained ovarian granulosa cells in POI group; A7: TUNEL stained ovarian granulosa cells in POI + EA group; A8: TUNEL stained ovarian granulosa cells in POI + EA + VP group; A9: merge of DAPI and TUNEL stained ovarian granulosa cells in control group; A10: merge of DAPI and TUNEL stained ovarian granulosa cells in POI group; A11: merge of DAPI and TUNEL stained ovarian granulosa cells in POI + EA group. A12: merge of DAPI and TUNEL stained ovarian granulosa cells in POI + EA + VP group. B: quantitative analysis of the TUNEL stained positive granulosa cells. C: the mitochondrial morphology was observed under a transmission electron microscope. C1: control group; C2: POI group; C3: POI + EA group; C4: POI + EA + VP group. D: the Western blotting assay was performed to access protein expression related to mitophagy. E: quantitative analysis of Pink1 protein levels. F: quantitative analysis of Parkin protein levels. G: quantitative analysis of LC3Ⅱ/Ⅰ protein levels. Control group: injected with normal saline. POI group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide. POI + EA group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide and the disposable sterile acupuncture needles were inserted into the Guanyuan (CV4) acupoint to a depth of 2 mm. The acupuncture needles were retained for 30 min per day for three weeks. POI + EA + VP group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide and the disposable sterile acupuncture needles were inserted into the Guanyuan (CV4) acupoint to a depth of 2 mm. The acupuncture needles were retained for 30 min per day for three weeks. During the acupuncture treatment, the mice were intraperitoneally injected with verteporfin (100 mg/kg body weight) every two days for three weeks. EA: electroacupuncture; TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling; DAPI: 4',6-diamidino-2-phenylindole; POI: premature ovarian insufficiency; VP: verteporfin; LC3: microtubule-associated protein 1 light chain 3. Statistical analyses were measured using one-way analysis of variance followed by Tukey's post hoc test. Data were presented as mean ± standard deviation (n = 6). Compared with the control group aP < 0.001; compared with the POI group, bP < 0.001; compared with the POI + EA group, cP < 0.01.
Figure 4 Verteporfin affects apoptosis, mitochondrial morphology and mitophagy of ovarian granulosa cells A: the TUNEL fluorescence staining assay was used to analyze apoptosis of granulosa cells. A1: DAPI stained ovarian granulosa cells in control group; A2: DAPI stained ovarian granulosa cells in POI group; A3: DAPI stained ovarian granulosa cells in POI + EA group; A4: DAPI stained ovarian granulosa cells in POI + EA + VP group; A5: TUNEL stained ovarian granulosa cells in control group; A6: TUNEL stained ovarian granulosa cells in POI group; A7: TUNEL stained ovarian granulosa cells in POI + EA group; A8: TUNEL stained ovarian granulosa cells in POI + EA + VP group; A9: merge of DAPI and TUNEL stained ovarian granulosa cells in control group; A10: merge of DAPI and TUNEL stained ovarian granulosa cells in POI group; A11: merge of DAPI and TUNEL stained ovarian granulosa cells in POI + EA group. A12: merge of DAPI and TUNEL stained ovarian granulosa cells in POI + EA + VP group. B: quantitative analysis of the TUNEL stained positive granulosa cells. C: the mitochondrial morphology was observed under a transmission electron microscope. C1: control group; C2: POI group; C3: POI + EA group; C4: POI + EA + VP group. D: the Western blotting assay was performed to access protein expression related to mitophagy. E: quantitative analysis of Pink1 protein levels. F: quantitative analysis of Parkin protein levels. G: quantitative analysis of LC3Ⅱ/Ⅰ protein levels. Control group: injected with normal saline. POI group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide. POI + EA group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide and the disposable sterile acupuncture needles were inserted into the Guanyuan (CV4) acupoint to a depth of 2 mm. The acupuncture needles were retained for 30 min per day for three weeks. POI + EA + VP group: single intraperitoneally injection with 12 mg/kg busulfan and 120 mg/kg cyclophosphamide and the disposable sterile acupuncture needles were inserted into the Guanyuan (CV4) acupoint to a depth of 2 mm. The acupuncture needles were retained for 30 min per day for three weeks. During the acupuncture treatment, the mice were intraperitoneally injected with verteporfin (100 mg/kg body weight) every two days for three weeks. EA: electroacupuncture; TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling; DAPI: 4',6-diamidino-2-phenylindole; POI: premature ovarian insufficiency; VP: verteporfin; LC3: microtubule-associated protein 1 light chain 3. Statistical analyses were measured using one-way analysis of variance followed by Tukey's post hoc test. Data were presented as mean ± standard deviation (n = 6). Compared with the control group aP < 0.001; compared with the POI group, bP < 0.001; compared with the POI + EA group, cP < 0.01.
| 1. |
Genes linked to premature ovarian insufficiency show no pathogenicity in the general population. Nat Med 2023; 29: 1617-18.
DOI PMID |
| 2. | Wang F, Chen X, Sun B, et al. Hypermethylation-mediated downregulation of lncRNA PVT1 promotes granulosa cell apoptosis in premature ovarian insufficiency via interacting with Foxo3a. J Cell Physiol 2021; 236: 5162-75. |
| 3. | Jin X, Wang K, Wang L, et al. RAB 7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging. Autophagy 2022; 18: 643-60. |
| 4. | Huang P, Zhou Y, Tang W, et al. Long-term treatment of Nicotinamide mononucleotide improved age-related diminished ovary reserve through enhancing the mitophagy level of granulosa cells in mice. J Nutr Biochem 2022; 101: 108911. |
| 5. | Li N, Xu H, Liu X, et al. Exposure to benzo(a)pyrene suppresses mitophagy via ANT1-PINK1-Parkin pathway in ovarian corpus luteum during early pregnancy. Sci Total Environ 2022; 814: 152759. |
| 6. | Hu X, Wang J, Chai J, et al. Chaetomugilin J enhances apoptosis in human ovarian cancer A2780 cells induced by cisplatin through inhibiting Pink1/Parkin mediated mitophagy. Onco Targets Ther 2020; 13: 9967-76. |
| 7. |
May-Panloup P, Boucret L, Chao De La Barca J, et al. Ovarian ageing: the role of mitochondria in oocytes and follicles. Hum Reprod Update 2016; 22: 725-43.
PMID |
| 8. | Luo Y, Xu D, Tang X, et al. Auricular acupuncture for premature ovarian insufficiency: a protocol for systematic review and Meta-analysis. Medicine (Baltimore) 2020; 99: e22212. |
| 9. | Chen M, He QD, Guo JJ, et al. Electro-acupuncture regulates metabolic disorders of the liver and kidney in premature ovarian failure mice. Front Endocrinol (Lausanne) 2022; 13: 882214. |
| 10. |
Xu Y, Zhang Y, He W, et al. Efficiency and safety of acupuncture for women with premature ovarian insufficiency: study protocol for a randomized controlled trial. J Tradit Chin Med 2023; 43: 1268-74.
DOI |
| 11. | Wang S, Lin S, Zhu M, et al. Acupuncture reduces apoptosis of Granulosa cells in rats with premature ovarian failure via restoring the PI3K/Akt signaling pathway. Int J Mol Sci 2019; 20: 6311. |
| 12. | Shen J, Li HX, Lu G, et al. Protective effect of moxibustion preconditioning in rats with premature ovarian insufficiency. Zhen Ci Yan Jiu 2023; 48: 267-73. |
| 13. | Hsu WT, Chen YH, Yang HB, Lin JG, Hung SY. Electroacupuncture improves motor symptoms of Parkinson's disease and promotes neuronal autophagy activity in mouse brain. Am J Chin Med 2020; 48: 1651-69. |
| 14. | Yu CC, Du YJ, Wang SQ, et al. Experimental evidence of the benefits of acupuncture for Alzheimer's disease: an updated review. Front Neurosci 2020; 14: 549772. |
| 15. | Umair Z, Baek MO, Song J, An S, Chon SJ, Yoon MS. MicroRNA-4516 in urinary exosomes as a biomarker of premature ovarian insufficiency. Cells 2022; 11: 2797. |
| 16. |
Liu M, Zhang D, Zhou X, et al. Cell-free fat extract improves ovarian function and fertility in mice with premature ovarian insufficiency. Stem Cell Res Ther 2022; 13: 320.
DOI PMID |
| 17. | Wang SC, Jiang YM, Qiu LR, Su M. Efficacy of needling acupoints of Guanyuan (CV4), Sanyinjiao (SP6), Zusanli (ST36), Pishu (BL20), Shenshu (BL23), Zigong (EX-CA1) on expression of p38 mitogen-activated protein kinase in ovarian tissue in rats with premature ovarian failure induced by cyclophosphamide. J Tradit Chin Med 2021; 41: 953-58. |
| 18. | Wei H, Wang F, Wang Y, et al. Verteporfin suppresses cell survival, angiogenesis and vasculogenic mimicry of pancreatic ductal adenocarcinoma via disrupting the YAP‐TEAD complex. Cancer Sci 2017; 108: 478-87. |
| 19. |
Chen X, Tang H, Liang Y, et al. Acupuncture regulates the autophagy of ovarian granulosa cells in polycystic ovarian syndrome ovulation disorder by inhibiting the PI3K/AKT/mTOR pathway through LncMEG3. Biomed Pharmacother 2021; 144: 112288.
DOI PMID |
| 20. |
Zhang H, Qin F, Liu A, et al. Electro-acupuncture attenuates the mice premature ovarian failure via mediating PI3K/AKT/mTOR pathway. Life Sci 2019; 217: 169-75.
DOI PMID |
| 21. | Wang D, Geng M, Gan D, et al. Effect of resveratrol on mouse ovarian vitrification and transplantation. Reprod Biol Endocrinol 2021; 19: 54. |
| 22. |
Takahashi A, Yousif A, Hong L, Chefetz I. Premature ovarian insufficiency: pathogenesis and therapeutic potential of mesenchymal stem cell. J Mol Med (Berl) 2021; 99: 637-50.
DOI PMID |
| 23. |
Xing J, Qiao G, Luo X, et al. Ferredoxin 1 regulates granulosa cell apoptosis and autophagy in polycystic ovary syndrome. Clin Sci (Lond) 2023; 137: 453-68.
DOI PMID |
| 24. | Shen HH, Zhang T, Yang HL, et al. Ovarian hormones-autophagy-immunity axis in menstruation and endometriosis. Theranostics 2021; 11: 3512-26. |
| 25. |
Zhou J, Peng X, Mei S. Autophagy in ovarian follicular development and atresia. Int J Biol Sci 2019; 15: 726-37.
DOI PMID |
| 26. | Zhu F, Gao J, Zeng F, Lai Y, Ruan X, Deng G. Hyperoside protects against cyclophosphamide induced ovarian damage and reduced fertility by suppressing HIF-1alpha/BNIP3-mediated autophagy. Biomed Pharmacother 2022; 156: 113743. |
| 27. | Wu X, Zheng Y, Liu M, et al. BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains. Autophagy 2021; 17: 1934-46. |
| 28. | Shen Q, Liu Y, Li H, Zhang L. Effect of mitophagy in oocytes and granulosa cells on oocyte qualitydagger. Biol Reprod 2021; 104: 294-304. |
| 29. | Sreerangaraja UD, Wu WH, Komrskova K, et al. Mitochondrial function in modulating human granulosa cell steroidogenesis and female fertility. Int J Mol Sci 2020; 21: 3592. |
| 30. | Wang X, Wang Y, Wei S, et al. An overview of systematic reviews of acupuncture for infertile women undergoing in vitro fertilization and embryo transfer. Front Public Health 2021; 9: 651811. |
| 31. | Nicolian S, Butel T, Gambotti L, et al. Cost-effectiveness of acupuncture versus standard care for pelvic and low back pain in pregnancy: a randomized controlled trial. PLoS One 2019; 14: e0214195. |
| 32. | Huang L, Chen Y, Luo M, Tang Y, Wei S. Acupuncture for patients with premature ovarian insufficiency: a systematic review protocol. Medicine (Baltimore) 2019; 98: e15444. |
| 33. | Liu P, Yu X, Dai X, et al. Scalp acupuncture attenuates brain damage after intracerebral hemorrhage through enhanced mitophagy and reduced apoptosis in rats. Front Aging Neurosci 2021; 13: 718631. |
| 34. | Li XT, Fang YG, Shang J, Guo XC. Ideas and exploration on acupuncture treatment of premature ovarian failure. Chin J Tradit Chin Med 2016; 31: 3170-2. |
| 35. | Yang NN, Yang JW, Ye Y, et al. Electroacupuncture ameliorates intestinal inflammation by activating alpha7nAChR-mediated JAK2/STAT3 signaling pathway in postoperative ileus. Theranostics 2021; 11: 4078-89. |
| 36. | Cao Y, Wang L, Lin LT, et al. Acupuncture attenuates cognitive deficits through alpha7nAChR mediated anti-inflammatory pathway in chronic cerebral hypoperfusion rats. Life Sci 2021; 266: 118732. |
| 37. |
Xie L, Wu S, Cao D, et al. Huyang Yangkun formula protects against 4-Vinylcyclohexene diepoxide-induced premature ovarian insufficiency in rats via the Hippo-JAK2/STAT3 signaling pathway. Biomed Pharmacother 2019; 116: 109008.
DOI |
| 38. | Wang Q, Wu X, Zhang J, et al. Role of ROS/JAK2/STAT3 signaling pathway in di-n-butyl phthalate-induced testosterone synthesis inhibition and antagonism of lycopene. Food Chem Toxicol 2023; 175: 113741. |
| 39. | Zhou XY, Zhang J, Li Y, et al. Advanced oxidation protein products induce G1/G0-phase arrest in ovarian granulosa cells via the ROS-JNK/p38 MAPK-p21 pathway in premature ovarian insufficiency. Oxid Med Cell Longev 2021; 2021: 6634718. |
| 40. | Mizutani T, Orisaka M, Kawabe S, Morichika R, Uesaka M, Yoshida Y. YAP/TAZ-TEAD is a novel transcriptional regulator of genes encoding steroidogenic enzymes in rat granulosa cells and KGN cells. Mol Cell Endocrinol 2023; 559: 111808. |
| 41. | Masciangelo R, Hossay C, Chiti MC, et al. Role of the PI3K and Hippo pathways in follicle activation after grafting of human ovarian tissue. J Assist Reprod Genet 2020; 37: 101-8. |
| 42. | Wang C, Sun H, Davis JS, et al. FHL2 deficiency impairs follicular development and fertility by attenuating EGF/ EGFR/YAP signaling in ovarian granulosa cells. Cell Death Dis 2023; 14: 239. |
| 43. |
Godin P, Tsoi MF, Morin M, Gevry N, Boerboom D. The granulosa cell response to luteinizing hormone is partly mediated by YAP1-dependent induction of amphiregulin. Cell Commun Signal 2022; 20: 72.
DOI PMID |
| 44. |
Ai A, Xiong Y, Wu B, et al. Induction of miR-15a expression by tripterygium glycosides caused premature ovarian failure by suppressing the Hippo-YAP/TAZ signaling effector Lats1. Gene 2018; 678: 155-63.
DOI PMID |
| [1] | LI Siting, WANG Shaojun, YIN Yehui, DE Gejing, LI Caicai, WANG Ziyan, CAO Wenjie. Electroacupuncture alleviates zymosan-induced colorectal hypersensitivity [J]. Journal of Traditional Chinese Medicine, 2025, 45(1): 32-38. |
| [2] | WU Jiaman, NING Yan, TAN Liya, MA Fei, LIN Yanting, ZHUO Yuanyuan. Difference of the gut microbiota of premature ovarian insufficiency in two traditional Chinese syndromes [J]. Journal of Traditional Chinese Medicine, 2025, 45(1): 132-139. |
| [3] | LANG Jiawang, JIN Lingqing, LUO Jianchang, LANG Boxu. Effects of acupuncture combined with bone-setting therapy to treat tourette syndrome: a three-arm randomized controlled trial [J]. Journal of Traditional Chinese Medicine, 2025, 45(1): 176-183. |
| [4] | Emre Bulut, Didem Özkal Eminoğlu, Yasemin Çayır. Effect of electroacupuncture on pain after periodontal flap surgery: a randomized controlled trial [J]. Journal of Traditional Chinese Medicine, 2025, 45(1): 184-191. |
| [5] | HE Ling, YANG Hui, LI Kang, WANG Junwen, SUN Zhibo, YANG Jinsheng, ZHANG Jing. Research on acupuncture robots based on the OptiTrack motion capture system and a robotic arm [J]. Journal of Traditional Chinese Medicine, 2025, 45(1): 201-212. |
| [6] | WANG Bingyu, JIN Fangfang, GAO Jiawei, YANG Liuxin, ZHANG Yali, YUAN Xingxing, ZHANG Yang. Acupuncture reduces sedative and anaesthetic consumption and improves pain tolerance in patients undergoing colonoscopy: a Meta-analysis [J]. Journal of Traditional Chinese Medicine, 2024, 44(6): 1091-1103. |
| [7] | ZHANG Boyang, ZHOU Yang, FENG Liyuan, SUI Dan, HE Lei, TONG Dan, WANG Ruoyu, SUI Xue, SONG Jing, WANG Dongyan. A neural regulation mechanism of head electroacupuncture on brain network of patients with stroke related sleep disorders [J]. Journal of Traditional Chinese Medicine, 2024, 44(6): 1268-1276. |
| [8] | XI Hanqing, LI Xia, ZHANG Ziyi, CUI Xiang, JING Xianghong, ZHU Bing, GAO Xinyan. Neuro- and immuno-modulation mediated by the cardiac sympathetic nerve: a novel insight into the anti-ischemic efficacy of acupuncture [J]. Journal of Traditional Chinese Medicine, 2024, 44(5): 1058-1066. |
| [9] | XU Yingshan, WU Chunxiao, YU Wei, GUO Hongji, LU Liming, XU Nenggui, TANG Chunzhi. Systematic review and Meta-analysis of brain plasticity associated with electroacupuncture in experimental ischemic stroke [J]. Journal of Traditional Chinese Medicine, 2024, 44(5): 859-870. |
| [10] | ZHENG Peng, MENG Ying, LIU Meijun, YU Di, LIU Huiying, WANG Fuchun, XU Xiaohong. Electroacupuncture inhibits hippocampal oxidative stress and autophagy in sleep-deprived rats through the protein kinase B and mechanistic target of rapamycin signaling pathway [J]. Journal of Traditional Chinese Medicine, 2024, 44(5): 974-980. |
| [11] | ZHANG Fang, YAN Cuina, WENG Zhijun, WU Luyi, QI Li, ZHAO Min, XIN Yuhu, WU Huangan, LIU Huirong. Regulatory role of electroacupuncture on satellite glial cell activity in the colon and dorsal root ganglion of rats with irritable bowel syndrome [J]. Journal of Traditional Chinese Medicine, 2024, 44(5): 981-990. |
| [12] | LI Zhongzheng, ZHAO Yadan, Ma Weigang, Zhang Yonglong, XU Zhifang, XI Qiang, LI Yanqi, QIN Siru, ZHANG Zichen, WANG Songtao, ZHAO Xue, LIU Yangyang, GUO Yi, GUO Yongming. Adenosine triphosphate mediates the pain tolerance effect of manual acupuncture at Zusanli (ST36) in mice [J]. Journal of Traditional Chinese Medicine, 2024, 44(4): 660-669. |
| [13] | YAN Jing, FENG Huimin, QIU Fang, WANG Haijun, YIN Luyun, JIN Xiaofei, ZHAO Jiyu, WANG Hongyang, YAN Xiaoqin. Effect on serum metabolomics of rats with premature ovarian insufficiency by Zhibian (BL54) through Shuidao (ST28) acupuncture [J]. Journal of Traditional Chinese Medicine, 2024, 44(4): 722-733. |
| [14] | CHEN Yonglin, OUYANG Ling, MENG Lingling, WU Bufan, PENG Rou, LIU Sitong, HOU Dan, WANG Yaling, JING Xinyue, LU Shengfeng, FU Shuping. Electroacupuncture ameliorates blood-brain barrier disruption after ischemic stroke through histone acetylation regulation at the matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 2 genes [J]. Journal of Traditional Chinese Medicine, 2024, 44(4): 734-744. |
| [15] | SUN Junjian, XIE Henghui, LI Huanhuan, TIAN Xiangming, FANG Yigong, ZHOU Wenhui. Acupuncture improves the live birth of patients with repeated implantation failure: a retrospective cohort study [J]. Journal of Traditional Chinese Medicine, 2024, 44(4): 830-838. |
| Viewed | ||||||
|
Full text |
|
|||||
|
Abstract |
|
|||||
