Journal of Traditional Chinese Medicine ›› 2025, Vol. 45 ›› Issue (1): 32-38.DOI: 10.19852/j.cnki.jtcm.20220425.001
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Electroacupuncture alleviates zymosan-induced colorectal hypersensitivity
LI Siting1, WANG Shaojun1(
), YIN Yehui1, DE Gejing2, LI Caicai1, WANG Ziyan1, CAO Wenjie1
- 1 Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China
2 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
-
Received:2022-01-16Accepted:2022-03-22Online:2025-02-15Published:2022-04-25 -
Contact:WANG Shaojun, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.ddwsj5@yeah.net , Telephone: +86-10-64089729 -
Supported by:the National Natural Science Foundation of China(Transient Potential Vanilloid Receptor1 Participates In The Mechanism Of Acupuncture Alleviating Visceral Hypersensitivity)(81373724);The Joint Innovation Funds of China Academy of Chinese Medical Sciences(Clinical observation of acupuncture in the treatment of chronic urticaria)(ZJYB2021-06)
Cite this article
LI Siting, WANG Shaojun, YIN Yehui, DE Gejing, LI Caicai, WANG Ziyan, CAO Wenjie. Electroacupuncture alleviates zymosan-induced colorectal hypersensitivity[J]. Journal of Traditional Chinese Medicine, 2025, 45(1): 32-38.
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Figure 1 EA attenuates zymosan-induced expression of TRPV1 and NGF in the colorectum. Protein levels were evaluated by Western blotting. The representative western blot diagram was shown in A and the statistical analysis diagram was shown in B. Both proteins were upregulated by zymosan injection (P < 0.05, S + C vs Z + C), an effect that was abrogated by EA (P < 0.05, Z + A vs Z + C). Anti-TrpV1 (Alomone Labs, Jerusalem, Israel; #Acc-030, 150KD, delusion: 1 : 800), anti-NGF (Santa Cruz Biotechnology, #sc-548, 30 kDa, delusion: 1:800). aP < 0.05 (versus S + C). bP < 0.05 (versus Z + C).
Figure 1 EA attenuates zymosan-induced expression of TRPV1 and NGF in the colorectum. Protein levels were evaluated by Western blotting. The representative western blot diagram was shown in A and the statistical analysis diagram was shown in B. Both proteins were upregulated by zymosan injection (P < 0.05, S + C vs Z + C), an effect that was abrogated by EA (P < 0.05, Z + A vs Z + C). Anti-TrpV1 (Alomone Labs, Jerusalem, Israel; #Acc-030, 150KD, delusion: 1 : 800), anti-NGF (Santa Cruz Biotechnology, #sc-548, 30 kDa, delusion: 1:800). aP < 0.05 (versus S + C). bP < 0.05 (versus Z + C).
Figure 2 EA attenuates zymosan-induced expression of TRPV1 and SP in the spinal ganglion. Protein levels were evaluated by western blotting. Protein levels were evaluated by western blotting. The representative western blot diagram was shown in A and the statistical analysis diagram was shown in B. The expression of both proteins was upregulated by zymosan injection (P < 0.05, S + C vs Z + C), but this effect was reversed by EA (P < 0.05, Z + A vs Z + C). The blot is representative of at least 3 independent experiments. Anti-SP (Santa Cruz Biotechnology, 18 kDa, #sc-9758, delusion: 1 : 700). aP < 0.05 (versus S + C). bP < 0.05 (versus Z + C).
Figure 2 EA attenuates zymosan-induced expression of TRPV1 and SP in the spinal ganglion. Protein levels were evaluated by western blotting. Protein levels were evaluated by western blotting. The representative western blot diagram was shown in A and the statistical analysis diagram was shown in B. The expression of both proteins was upregulated by zymosan injection (P < 0.05, S + C vs Z + C), but this effect was reversed by EA (P < 0.05, Z + A vs Z + C). The blot is representative of at least 3 independent experiments. Anti-SP (Santa Cruz Biotechnology, 18 kDa, #sc-9758, delusion: 1 : 700). aP < 0.05 (versus S + C). bP < 0.05 (versus Z + C).
Figure 3 EA attenuates zymosan-induced expression of TRPV1 and PKCγ the in spinal cord. Protein levels were evaluated by western blotting The representative western blot diagram was shown in A and the statistical analysis diagram was shown in B. Both proteins were upregulated by zymosan injection (P < 0.05, S + C vs Z + C), but the increase was abolished by EA. The blot is representative of at least 3 independent experiments. PKCγ (Millipore; 75kD, #05983, delusion: 1 : 800). aP < 0.05 (versus S + C). bP < 0.05 (versus Z + C).
Figure 3 EA attenuates zymosan-induced expression of TRPV1 and PKCγ the in spinal cord. Protein levels were evaluated by western blotting The representative western blot diagram was shown in A and the statistical analysis diagram was shown in B. Both proteins were upregulated by zymosan injection (P < 0.05, S + C vs Z + C), but the increase was abolished by EA. The blot is representative of at least 3 independent experiments. PKCγ (Millipore; 75kD, #05983, delusion: 1 : 800). aP < 0.05 (versus S + C). bP < 0.05 (versus Z + C).
Figure 4 TRPV1/IB4 double immunofluorescence labeling of neurons in the spinal ganglion Coexpression of TRPV1 (red) and IB4 (green) in neurons can be seen in the merged images (yellow). Representative neurons are shown enlarged in the inset in the upper-left corner of each panel. Arrowheads indicate immunopositive neurons. Scale bar, 100 μm. Anti-IB4 (Sigma-Aldrich; #L2895, delusion: 1 : 500).
Figure 4 TRPV1/IB4 double immunofluorescence labeling of neurons in the spinal ganglion Coexpression of TRPV1 (red) and IB4 (green) in neurons can be seen in the merged images (yellow). Representative neurons are shown enlarged in the inset in the upper-left corner of each panel. Arrowheads indicate immunopositive neurons. Scale bar, 100 μm. Anti-IB4 (Sigma-Aldrich; #L2895, delusion: 1 : 500).
Figure 5 TRPV1/CGRP double immunofluorescence labeling of neurons in the spinal ganglion Coexpression of TRPV1 (red) and CGRP (green) in neurons can be seen in the merged images (yellow). Representative neurons are shown enlarged in the inset in the upper-left corner of each panel. Arrowheads indicate immunopositive neurons. Scale bar, 100 μm. Goat anti-mouse CGRP (Pierce, Rockford, IL, USA; #PA1-85250, delusion: 1∶400).
Figure 5 TRPV1/CGRP double immunofluorescence labeling of neurons in the spinal ganglion Coexpression of TRPV1 (red) and CGRP (green) in neurons can be seen in the merged images (yellow). Representative neurons are shown enlarged in the inset in the upper-left corner of each panel. Arrowheads indicate immunopositive neurons. Scale bar, 100 μm. Goat anti-mouse CGRP (Pierce, Rockford, IL, USA; #PA1-85250, delusion: 1∶400).
Figure 6 TRPV1/NF200 double immunofluorescence labeling of neurons in the spinal ganglion. Coexpression of TRPV1 (red) and NF200 (green) in neurons can be seen in the merged images (yellow). Representative neurons are shown enlarged in the inset in the upper-left corner of each panel. Arrowheads indicate immunopositive neurons. Scale bar, 100 μm. NF200 (Abcam; #ab4680, delusion: 1∶500).
Figure 6 TRPV1/NF200 double immunofluorescence labeling of neurons in the spinal ganglion. Coexpression of TRPV1 (red) and NF200 (green) in neurons can be seen in the merged images (yellow). Representative neurons are shown enlarged in the inset in the upper-left corner of each panel. Arrowheads indicate immunopositive neurons. Scale bar, 100 μm. NF200 (Abcam; #ab4680, delusion: 1∶500).
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