Journal of Traditional Chinese Medicine ›› 2024, Vol. 44 ›› Issue (5): 974-980.DOI: 10.19852/j.cnki.jtcm.20240806.002
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Electroacupuncture inhibits hippocampal oxidative stress and autophagy in sleep-deprived rats through the protein kinase B and mechanistic target of rapamycin signaling pathway
ZHENG Peng1, MENG Ying2, LIU Meijun2, YU Di2, LIU Huiying2, WANG Fuchun2, XU Xiaohong2(
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- 1 Department of Neurology, The Third Affiliated Clinical Hospital of Changchun University of Chinese Medicine, Changchun 130000, China
2 Rehabilitation Medicine Academy, Changchun University of Chinese Medicine, Changchun 130117, China
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Received:2023-04-22Accepted:2023-10-17Online:2024-10-15Published:2024-08-06 -
Contact:Prof. XU Xiaohong, Rehabilitation Medicine Academy, Changchun University of Chinese Medicine, Changchun 130117, China. 740761229@qq.com Telephone: +86-13074319344 -
Supported by:Investigation of the Regulatory Mechanism of Cognitive Function in Sleep-Deprived Rats Treated with Acupuncture based on the Rat Sarcoma Virus Oncogene/Rapidly Accelerated Fibrosarcoma/Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase Kinase/Extracellular Signal-Regulated Kinase pathway(20210101222JC)
Cite this article
ZHENG Peng, MENG Ying, LIU Meijun, YU Di, LIU Huiying, WANG Fuchun, XU Xiaohong. Electroacupuncture inhibits hippocampal oxidative stress and autophagy in sleep-deprived rats through the protein kinase B and mechanistic target of rapamycin signaling pathway[J]. Journal of Traditional Chinese Medicine, 2024, 44(5): 974-980.
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Figure 1 Comparison of spatial learning and memory abilities among groups A: time spent in the target quadrant in each group (n = 3); electroacupuncture (20 Hz, 1 mA) was applied 15 min/d for 7 d. B: number of times each group entered the target quadrant position (n = 3). One-way analysis of variance was used for comparisons between multiple groups. If the overall difference was statistically significant, the least significant difference t-test was used for further pairwise comparisons. The data are expressed as mean ± standard deviation. Compared with the normal group, aP < 0.05; compared with the sleep deprivation group, bP < 0.05.
Figure 1 Comparison of spatial learning and memory abilities among groups A: time spent in the target quadrant in each group (n = 3); electroacupuncture (20 Hz, 1 mA) was applied 15 min/d for 7 d. B: number of times each group entered the target quadrant position (n = 3). One-way analysis of variance was used for comparisons between multiple groups. If the overall difference was statistically significant, the least significant difference t-test was used for further pairwise comparisons. The data are expressed as mean ± standard deviation. Compared with the normal group, aP < 0.05; compared with the sleep deprivation group, bP < 0.05.
Figure 2 Comparison of oxidative stress levels in the brains of rats in each group A: MDA content in the brain tissue of rats in each group (n = 3), electroacupuncture (20 Hz, 1 mA) was applied 15 min/d for 7 d; B: SOD content in the brain tissue of rats in each group; C: GPX content in the brain tissue of rats in each group. MDA: malondialdehyde; SOD: superoxide dismutase; GPX: glutathione peroxidase. One-way analysis of variance was used for comparisons between multiple groups. If the overall difference was statistically significant, the least significant difference t-test was used for further pairwise comparisons. The data are expressed as mean ± standard deviation. Compared with the normal group, aP < 0.01; compared with sleep deprivation group, bP < 0.05.
Figure 2 Comparison of oxidative stress levels in the brains of rats in each group A: MDA content in the brain tissue of rats in each group (n = 3), electroacupuncture (20 Hz, 1 mA) was applied 15 min/d for 7 d; B: SOD content in the brain tissue of rats in each group; C: GPX content in the brain tissue of rats in each group. MDA: malondialdehyde; SOD: superoxide dismutase; GPX: glutathione peroxidase. One-way analysis of variance was used for comparisons between multiple groups. If the overall difference was statistically significant, the least significant difference t-test was used for further pairwise comparisons. The data are expressed as mean ± standard deviation. Compared with the normal group, aP < 0.01; compared with sleep deprivation group, bP < 0.05.
Figure 3 Comparison of autophagy levels in the brains of rats in each group A: representative images of LC3A Ⅱ/Ⅰ ratio, LC3B Ⅱ/Ⅰ ratio and relative expression of Beclin 1 in the normal, sleep deprivation and acupuncture groups (n = 3). Electroacupuncture (20 Hz, 1 mA) was applied 15 min/d for 7 d. B: LC3A Ⅱ/Ⅰ ratio, LC3B Ⅱ/Ⅰ ratio and relative expression of Beclin 1 in the hippocampal tissues of rats in each group. LC3A: Light chain 3 alpha; LC3B: Light chain 3 beta. One-way analysis of variance was used for comparisons between multiple groups. If the overall difference was statistically significant, the least significant difference t-test was used for further pairwise comparisons. Data were presented as mean ± standard deviation (n = 3). Compared with the normal group, aP < 0.01; compared with sleep deprivation group, bP < 0.05.
Figure 3 Comparison of autophagy levels in the brains of rats in each group A: representative images of LC3A Ⅱ/Ⅰ ratio, LC3B Ⅱ/Ⅰ ratio and relative expression of Beclin 1 in the normal, sleep deprivation and acupuncture groups (n = 3). Electroacupuncture (20 Hz, 1 mA) was applied 15 min/d for 7 d. B: LC3A Ⅱ/Ⅰ ratio, LC3B Ⅱ/Ⅰ ratio and relative expression of Beclin 1 in the hippocampal tissues of rats in each group. LC3A: Light chain 3 alpha; LC3B: Light chain 3 beta. One-way analysis of variance was used for comparisons between multiple groups. If the overall difference was statistically significant, the least significant difference t-test was used for further pairwise comparisons. Data were presented as mean ± standard deviation (n = 3). Compared with the normal group, aP < 0.01; compared with sleep deprivation group, bP < 0.05.
Figure 4 Phosphorylation level of the AKT/mTOR/p70S6K signaling pathway in rat hippocampal tissue A: Western blotting was performed to analyze the expression of AKT, mTOR and p70S6K in the hippocampal tissues (n = 3). Electroacupuncture (20 Hz, 1 mA) was applied 15 min/d for 7 d. B: quantitative analysis of AKT, mTOR and p70S6K protein. AKT: protein kinase B; mTOR: mechanistic target of rapamycin. One-way analysis of variance was used for comparisons between multiple groups. If the overall difference was statistically significant, the least significant difference t-test was used for further pairwise comparisons. Data were presented as mean ± standard deviation (n = 3). Compared with the normal group, aP < 0.01; compared with the sleep deprivation group, bP < 0.05.
Figure 4 Phosphorylation level of the AKT/mTOR/p70S6K signaling pathway in rat hippocampal tissue A: Western blotting was performed to analyze the expression of AKT, mTOR and p70S6K in the hippocampal tissues (n = 3). Electroacupuncture (20 Hz, 1 mA) was applied 15 min/d for 7 d. B: quantitative analysis of AKT, mTOR and p70S6K protein. AKT: protein kinase B; mTOR: mechanistic target of rapamycin. One-way analysis of variance was used for comparisons between multiple groups. If the overall difference was statistically significant, the least significant difference t-test was used for further pairwise comparisons. Data were presented as mean ± standard deviation (n = 3). Compared with the normal group, aP < 0.01; compared with the sleep deprivation group, bP < 0.05.
| 1. |
Iranzo A. An overview on sleep medicine. Adv Exp Med Biol 2022; 1384: 3-15.
DOI PMID |
| 2. | Melhuish Beaupre LM, Brown GM, Braganza NA, Kennedy JL, Gonçalves VF. Mitochondria's role in sleep: novel insights from sleep deprivation and restriction studies. World J Biol Psychiatry 2022; 23: 1-13. |
| 3. |
Luo F, Han S, Xia M, Chen Z, Liu P, Lin J. Study on the mechanism of hydrolyzed seawater pearl tablet in treating chronic sleep deprivation mice model. Endocr Metab Immune Disord Drug Targets 2023; 23: 927-36.
DOI PMID |
| 4. | Alrousan G, Hassan A, Pillai AA, Atrooz F, Salim S. Early life sleep deprivation and brain development: insights from human and animal studies. Front Neurosci 2022; 16: 833786. |
| 5. |
Tobaldini E, Costantino G, Solbiati M, et al. Sleep, sleep deprivation, autonomic nervous system and cardiovascular diseases. Neurosci Biobehav Rev 2017; 74: 321-9.
DOI PMID |
| 6. |
Eide PK, Vinje V, Pripp AH, Mardal KA, Ringstad G. Sleep deprivation impairs molecular clearance from the human brain. Brain 2021; 144: 863-74.
DOI PMID |
| 7. | Bedont JL, Toda H, Shi M, et al. Short and long sleeping mutants reveal links between sleep and macroautophagy. Elife 2021; 10: e64140. |
| 8. |
Xie Y, Ba L, Wang M, et al. Chronic sleep fragmentation shares similar pathogenesis with neurodegenerative diseases: Endosome-autophagosome-lysosome pathway dysfunction and microglia-mediated neuroinflammation. CNS Neurosci Ther 2020; 26: 215-27.
DOI PMID |
| 9. | Yun HR, Jo YH, Kim J, Shin Y, Kim SS, Choi TG. Roles of autophagy in oxidative stress. Int J Mol Sci 2020; 21: 3289. |
| 10. | Cao Y, Li Q, Liu L, et al. Modafinil protects hippocampal neurons by suppressing excessive autophagy and apoptosis in mice with sleep deprivation. Br J Pharmacol 2019; 176: 1282-97. |
| 11. |
Frolinger T, Smith C, Cobo CF, et al. Dietary polyphenols promote resilience against sleep deprivation-induced cognitive impairment by activating protein translation. FASEB J 2018; 32: 5390-404.
DOI PMID |
| 12. | Sun W, Li J, Cui S, et al. Sleep deprivation disrupts acquisition of contextual fear extinction by affecting circadian oscillation of hippocampal-Infralimbic proBDNF. eNeuro 2019; 6: ENEURO.0165-19.2019. |
| 13. | Hao L, Wu Y, Xie J, Chen X. Electroacupuncture enhances cognitive deficits in a rat model of rapid eye movement sleep deprivation via targeting MiR-132. Evid Based Complement Alternat Med 2022; 2022: 7044208. |
| 14. | Chen D, Zhang Y, Wang C, et al. Modulation of hippocampal dopamine and synapse-related proteins by electroacupuncture improves memory deficit caused by sleep deprivation. Acupunct Med 2020; 38: 343-51. |
| 15. | Gao L, Zhang M, Gong H, et al. Differential activation patterns of FMRI in sleep-deprived brain: restoring effects of acupuncture. Evid Based Complement Alternat Med 2014; 2014: 465760. |
| 16. |
Yan B, Wang FC, Ma TS, et al. Efficacy and safety of electroacupuncture treatment in the prevention of negative moods in healthy young men after 30 h of total sleep deprivation: study protocol for a single-center, single-blind, parallel-arm, randomized clinical trial. Trials 2021; 22: 761.
DOI PMID |
| 17. | Yeung WF, Yu BY, Yuen JW, et al. Semi-individualized acupuncture for insomnia disorder and oxidative stress: a randomized, double-blind, sham-controlled trial. Nat Sci Sleep 2021; 13: 1195-207. |
| 18. | Zheng P, Xu X, Zhao H, Lyu T, Song B, Wang F. Tranquilizing and allaying excitement needling method affects BDNF and SYP expression in hippocampus. Evid Based Complement Alternat Med 2017; 2017: 8215949. |
| 19. | Xu X, Zheng P, Zhao H, Song B, Wang F. Effect of electroacupuncture at GV20 on sleep deprivation-induced depression-like behavior in mice. Evid Based Complement Alternat Med 2020; 2020: 7481813. |
| 20. |
Pei W, Meng F, Deng Q, et al. Electroacupuncture promotes the survival and synaptic plasticity of hippocampal neurons and improvement of sleep deprivation-induced spatial memory impairment. CNS Neurosci Ther 2021; 27: 1472-82.
DOI PMID |
| 21. |
Machado RB, Suchecki D, Tufik S. Comparison of the sleep pattern throughout a protocol of chronic sleep restriction induced by two methods of paradoxical sleep deprivation. Brain Res Bull 2006; 70: 213-20.
PMID |
| 22. |
Hudson AN, Van Dongen HPA, Honn KA. Sleep deprivation, vigilant attention, and brain function: a review. Neuropsychopharmacology 2020; 45: 21-30.
DOI PMID |
| 23. | Ning Y, Zheng S, Feng S, et al. The altered intrinsic functional connectivity after acupuncture at shenmen (HT7) in acute sleep deprivation. Front Neurol 2022; 13: 947379. |
| 24. | Dai XJ, Min YJ, Gong HH, et al. Evaluation of the post-effect of acupuncture at Sanyinjiao (SP6) under sleep deprivation by resting-state amplitude of low-frequency fluctuation: a fMRI study. Zhong Guo Zhen Jiu 2012; 32: 47-52. |
| 25. |
Xue R, Wan Y, Sun X, Zhang X, Gao W, Wu W. Nicotinic mitigation of neuroinflammation and oxidative stress after chronic sleep deprivation. Front Immunol 2019; 10: 2546.
DOI PMID |
| 26. |
Valvassori SS, Resende WR, Dal-Pont G, et al. Lithium ameliorates sleep deprivation-induced mania-like behavior, hypothalamic-pituitary-adrenal (HPA) axis alterations, oxidative stress and elevations of cytokine concentrations in the brain and serum of mice. Bipolar Disord 2017; 19: 246-58.
DOI PMID |
| 27. |
Nabaee E, Kesmati M, Shahriari A, Khajehpour L, Torabi M. Cognitive and hippocampus biochemical changes following sleep deprivation in the adult male rat. Biomed Pharmacother 2018; 104: 69-76.
DOI PMID |
| 28. | Seo SY, Ryu Y. Electroacupuncture stimulation of HT7 alleviates sleep disruption following acute caffeine exposure by regulating BDNF-mediated endoplasmic reticulum stress in the rat medial septum. Biomed Pharmacother 2022; 155: 113724. |
| 29. |
D'Arcy MS. Cell death: a review of the major forms of apoptosis, necrosis and autophagy. Cell Biol Int 2019; 43: 582-92.
DOI PMID |
| 30. | Cheng WJ, Li P, Huang WY, et al. Acupuncture relieves stress-induced depressive behavior by reducing oxidative stress and neuroapoptosis in rats. Front Behav Neurosci 2022; 15: 783056. |
| 31. |
Havekes R, Abel T. The tired hippocampus: the molecular impact of sleep deprivation on hippocampal function. Curr Opin Neurobiol 2017; 44: 13-9.
DOI PMID |
| 32. | Cao Y, Li Q, Zhou A, et al. Notoginsenoside R1 reverses abnormal autophagy in hippocampal neurons of mice with sleep deprivation through melatonin receptor 1A. Front Pharmacol 2021; 12: 719313. |
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