Journal of Traditional Chinese Medicine ›› 2024, Vol. 44 ›› Issue (4): 753-761.DOI: 10.19852/j.cnki.jtcm.20240610.003

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Study on the anti-inflammatory mechanism of moxibustion in rheumatoid arthritis in rats based on phospholipaseA2 signaling inhibition by Annexin 1

GUO Yanding1, LUO Kun1, ZHANG Linlin1, LU Wenting3, SHANG Yanan1, ZHONG Yumei2, HU Danhui1, YANG Xin4, ZHOU Haiyan1()   

  1. 1 Department of Acupuncture-Moxibustion and Tuina, the Third Affiliated Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China
    2 Department of Chengdu Integrated Traditional Chinese Medicine and Western Medicine Hospital, Chengdu 610075, China
    3 External Treatment Center, First People's Hospital of Chengdu, Chengdu 610095, China
    4 Department of Health Rehabilitation School, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China
  • Received:2023-02-12 Accepted:2023-07-20 Online:2024-08-15 Published:2024-06-10
  • Contact: ZHOU Haiyan, Acupuncture and Moxibustion College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China. zhouhaiyan@cdutcm.edu.cn Telephone: +86-13551039390
  • Supported by:
    National Natural Science Foundation of China: Study on the Immune Mechanism of Macrophages 1/Macrophages 2 Polarization of Macrophages Treated with Moxibustion "Strengthening the Body and Expelling Pathogenic Qi" for "Struggle Between Healthy Qi and Pathogenic Qi" in Rheumatoid Arthritis(81973959);National Key R&D Program of China: a Comparative Study of Moxibustion at Zusanli (ST36) on the Polarization of Knee Synovial Macrophage in Kneeosteoar-thritis and Rheumatoid Arthritis Rat Models(2019YFC1709001);National Natural Science Foundation of China: Study on the Regulatory Mechanism of the "Immune-Inflammation" Molecular Signal of the Nucleotide—Binding Oligomerization Domain, Leucine rich Repeat and Pyrin domain containing Proteins 3 Inflammasome Treated by Moxibustion for Rheumatoid Arthritis(81774435);Foundation of Chengdu University of Traditional Chinese Medicine: Study on the Mechanism of " Macrophage Migration Inhibitory Factor -Target Protein-Glucocorticoid-Inflammation" in Moxibustion Treatment and Anti-inflammatory Effect of Rheumatoid Arthritis(QNXZ2018034);Science and Technology Innovation Seedling Project of Sichuan Province: Exploring the Effector Mechanism of Moxibustion Treatment in the Experimental Rheumatoid Arthritis Model based on the Macrophage Macrophages 1 Polarization Signaling Pathway Toll-like Receptors 4-Maximum Tolerated Dose 88- Nuclear Factor-Kappa B and its Regulatory Molecule T Cell Immunoglobulin and Mucin Domain-containing Protein 3(2022037)

Abstract:

OBJECTIVE: To determine whether moxibustion had an anti-inflammatory effect on rheumatoid arthritis (RA) by regulating Annexin 1 expression and interfering with the phospholipaseA2 signaling pathway.

METHODS: Thirty male Sprague-Dawley rats were randomly categorized into five groups (six rats per group): blank control (CON) group, RA model (RA) group, moxibustion (MOX) group, Annexin 1 lentiviral intervention (RNAi-Anxa1) group, and Annexin 1 lentiviral intervention + moxibustion (RNAi-Anxa1 + MOX) group. The rats in the RNAi-Anxa1 and the RNAi-Anxa1 + MOX groups were injected with the lentiviral vector-mediated RNAi-Anxa1 into the rat foot pad. An experimental RA rat model was established by injecting Freund's complete adjuvant (FCA) into the RA, MOX, RNAi-Anxa1, and RNAi-Anxa1 + MOX groups. Rats in the MOX and RNAi-Anxa1 + MOX groups received moxibustion treatment. After modeling, using moxibustion “Shenshu (BL23)” and “Zusanli (ST36)”, each point is 5 times, bilateral alternating, once a day, 6 times for a course of treatment, between the courses of rest for a one day. A total of three treatment courses were conducted. Both bilateral pad thicknesses were measured using Vernier calipers on experimental days 1, 7, 14, 21, and 28. The expression of cPLA2α signaling in the synovium of diseased joints was observed using Western blot. The pathology of the rat ankle synovium was observed using hematoxylin-eosin (HE) staining. Interleukin (IL)-1β, IL-10, prostaglandin E2 (PGE2), and leukotriene B4 (LTB4) were detected using enzyme-linked immunosorbent assay.

RESULTS: Moxibustion increased the levels of Annexin 1 and decreased the inflammatory response in rats with RA. After increasing the expression of Annexin 1, the phosphorylated expression of cPLA2α was inhibited, the serum levels of IL-1β, PGE2, and LTB4 decreased, and the level of IL-10 increased. In moxibustion treated RA rats after the Annexin 1 lentiviral intervention, the serum levels of IL-1β, PGE2, LTB4, and IL-10 were almost unchanged.

CONCLUSION: Moxibustion enhanced the negative regulation of the cPLA2α signaling pathway, increased the synovial Annexin 1 expression, inhibited the cPLA2α signaling pathway, indirectly inhibited the expression of downstream inflammatory factors, and played a role in reducing inflammation.

Key words: annexins, group IV phospholipases A2, signal transduction, moxibustion, arthritis, rheumatoid

Cite this article

GUO Yanding, LUO Kun, ZHANG Linlin, LU Wenting, SHANG Yanan, ZHONG Yumei, HU Danhui, YANG Xin, ZHOU Haiyan. Study on the anti-inflammatory mechanism of moxibustion in rheumatoid arthritis in rats based on phospholipaseA2 signaling inhibition by Annexin 1[J]. Journal of Traditional Chinese Medicine, 2024, 44(4): 753-761.