Journal of Traditional Chinese Medicine ›› 2024, Vol. 44 ›› Issue (4): 745-752.DOI: 10.19852/j.cnki.jtcm.2024.04.002
• Original articles • Previous Articles Next Articles
Effect of moxibustion on expression profile of miRNAs in Tripterygium glycoside-induced decreased ovarian reserve
SHEN Jie, YIN Yaoli, LI Hongxiao, LU Ge, ZHU Yaoyao, QIN Yantong, JIN Xun, CHENG Jie(
), SHEN Meihong()
- College of Acupuncture and Tuina, Nanjing University of Chinese Medicine, Nanjing 210023, China
-
Received:2023-03-02Accepted:2023-07-20Online:2024-08-15Published:2024-07-15 -
Contact:Prof. CHENG Jie, College of Acupuncture and Tuina, Nanjing University of Chinese Medicine, Nanjing 210023, China.grace@njucm.edu.cn ; Prof. SHEN Meihong, College of Acupuncture and Tuina, Nanjing University of Chinese Medicine, Nanjing 210023, China.mhshen@njucm.edu.cn Telephone: +86-13813963632 -
Supported by:National Natural Science Foundation of China: Based on Phosphatidylinositol-3-kinase/Protein kinase B Signaling Pathway Study microRNAs Regulating Mechanism of Moxibustion Improve Ovarian Function(81804179);Explore the miRNA Mechanism of Acupuncture and Moxibustion Against Oxygen Stress in Improving Ovarian Function based on Nuclear Factor Erythropoietin-2-related Factor 2/AU-rich Element Pathway(81774408);Explore the Effect and Molecular Mechanism of Moxibustion on Granulosa Cells in Premature Ovarian Failure based on "Autophagy-apoptosis Cross-talk"(81973957)
Cite this article
SHEN Jie, YIN Yaoli, LI Hongxiao, LU Ge, ZHU Yaoyao, QIN Yantong, JIN Xun, CHENG Jie, SHEN Meihong. Effect of moxibustion on expression profile of miRNAs in Tripterygium glycoside-induced decreased ovarian reserve[J]. Journal of Traditional Chinese Medicine, 2024, 44(4): 745-752.
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Figure 1 Moxibustion has a protective effect on ovarian damage in TGS-induced DOR rats A-C: hematoxylin and eosin staining was used to detect the morphological changes in ovarian tissue. A1-C1: ×40. A2-C2: Enlarged part (×100). A-C: control, model, and moxibustion. Control group: rats treated with intragastric physiological saline (10 mL·kg-1·d-1) for 14 d; Model group: rats treated with TGS suspension (50 mg·kg-1·d-1) for 14 d; Moxibustion group: rats treated with TGS suspension (50 mg·kg-1·d-1) and moxibustion for 14 d. PF: primary follicle; SF: secondary follicle; MF: mature follicle; AF: atretic follicle; circles represent corpus luteum. D: number of follicles in each ovarian stage. The difference between the two groups was measured by Student’s t-test. Data are shown as the mean?±?standard deviation (n = 6). aP < 0.01, compared with the control group; bP < 0.05 and cP < 0.05, compared with the model group. DOR: decreased ovarian reserve; TGS: Tripterygium glycoside.
Figure 1 Moxibustion has a protective effect on ovarian damage in TGS-induced DOR rats A-C: hematoxylin and eosin staining was used to detect the morphological changes in ovarian tissue. A1-C1: ×40. A2-C2: Enlarged part (×100). A-C: control, model, and moxibustion. Control group: rats treated with intragastric physiological saline (10 mL·kg-1·d-1) for 14 d; Model group: rats treated with TGS suspension (50 mg·kg-1·d-1) for 14 d; Moxibustion group: rats treated with TGS suspension (50 mg·kg-1·d-1) and moxibustion for 14 d. PF: primary follicle; SF: secondary follicle; MF: mature follicle; AF: atretic follicle; circles represent corpus luteum. D: number of follicles in each ovarian stage. The difference between the two groups was measured by Student’s t-test. Data are shown as the mean?±?standard deviation (n = 6). aP < 0.01, compared with the control group; bP < 0.05 and cP < 0.05, compared with the model group. DOR: decreased ovarian reserve; TGS: Tripterygium glycoside.
Figure 2 Effects of moxibustion on DOR on a transcriptome-wide scale A: volcano map of miRNA of the model versus the control groups; B: volcano map of miRNA of the moxibustion versus the model groups; C: venn diagram of the model versus the control (up) and the moxibustion versus the model (down) groups; D: venn diagram of the model versus the control (down) and the moxibustion versus the model (up) groups. Control group: rats treated with intragastric physiological saline (10 mL·kg-1·d-1) for 14 d; Model group: rats treated with TGS suspension (50 mg·kg-1·d-1) for 14 d; Moxibustion group: rats treated with TGS suspension (50 mg·kg-1·d-1) and moxibustion for 14 d. Mod: model; Con: control; Mox: moxibustion; DOR: decreased ovarian reserve; TGS: Tripterygium glycoside. The difference between the two groups was measured by Student’s t-test. Data are shown as the mean?±?standard deviation (n = 3).
Figure 2 Effects of moxibustion on DOR on a transcriptome-wide scale A: volcano map of miRNA of the model versus the control groups; B: volcano map of miRNA of the moxibustion versus the model groups; C: venn diagram of the model versus the control (up) and the moxibustion versus the model (down) groups; D: venn diagram of the model versus the control (down) and the moxibustion versus the model (up) groups. Control group: rats treated with intragastric physiological saline (10 mL·kg-1·d-1) for 14 d; Model group: rats treated with TGS suspension (50 mg·kg-1·d-1) for 14 d; Moxibustion group: rats treated with TGS suspension (50 mg·kg-1·d-1) and moxibustion for 14 d. Mod: model; Con: control; Mox: moxibustion; DOR: decreased ovarian reserve; TGS: Tripterygium glycoside. The difference between the two groups was measured by Student’s t-test. Data are shown as the mean?±?standard deviation (n = 3).
Figure 3 Enrichment analysis of differential miRNA A: GO terms analysis of differential miRNA; B: pathway analysis of differential miRNA. GO: Gene Ontology; PI3K-Akt: phosphatidylinositol-3-kinase-protein kinase B; MAPK: mitogen-activated protein kinase; Nrf2/HO-1: nuclear factor erythropoietin-2-related factor 2/heme oxygenase 1; AMPK: adenosine 5‘-monophosphate (AMP)-activated protein kinase; GPI: glycosylphosphatidylinositol, mTOR: mammalian target of rapamycin; FDR: false discovery rate.
Figure 3 Enrichment analysis of differential miRNA A: GO terms analysis of differential miRNA; B: pathway analysis of differential miRNA. GO: Gene Ontology; PI3K-Akt: phosphatidylinositol-3-kinase-protein kinase B; MAPK: mitogen-activated protein kinase; Nrf2/HO-1: nuclear factor erythropoietin-2-related factor 2/heme oxygenase 1; AMPK: adenosine 5‘-monophosphate (AMP)-activated protein kinase; GPI: glycosylphosphatidylinositol, mTOR: mammalian target of rapamycin; FDR: false discovery rate.
Figure 4 Construction of the miRNA-target gene-pathway and validation of miRNAs A: the network of miRNAs-target genes-pathway. The square represents the pathway; the diamond represents the mRNA; the octagon represents the miRNA. B: the differentially expressed miRNA was verified by RT-PCR. RT-PCR: reverse transcription polymerase chain reaction; Nrf2/ARE: nuclear factor erythropoietin-2-related factor 2/AU-rich element; PI3K/Akt: phosphatidylinositol-3-kinase/protein kinase B; Cdx2: caudal type homeobox 2; E2f1: E2 promoter binding factor 1; Atp5c1: adenosine triphosphate synthase subunit gamma; Sall1: spalt like transcription factor 1; Klf5: krüppel-like factor 5; Edn1: endothelin 1; Zfp382: zinc finger protein 382; Hmg20a: high mobility Group 20a; Bach2: BTB domain and CNC homolog 2; Bcl6b: B-cell Lymphoma 6b; Rnf2: ring finger protein 2; Cbx2: chromobox 2; Esr1: estrogen receptor 1; Shox2: short Stature homobox 2; Cav1: caveolin-1; Pias1: protein inhibitor of activated signal transducer and activators of transcription 1; Dcaf1: DDB1 and CUL4 associated factor 1. The difference between the two groups was measured by Student’s t-test. Data are shown as the mean?±?standard deviation (n = 5). aP < 0.05, compared with the control group. bP < 0.01 and cP < 0.05, compared with the model group.
Figure 4 Construction of the miRNA-target gene-pathway and validation of miRNAs A: the network of miRNAs-target genes-pathway. The square represents the pathway; the diamond represents the mRNA; the octagon represents the miRNA. B: the differentially expressed miRNA was verified by RT-PCR. RT-PCR: reverse transcription polymerase chain reaction; Nrf2/ARE: nuclear factor erythropoietin-2-related factor 2/AU-rich element; PI3K/Akt: phosphatidylinositol-3-kinase/protein kinase B; Cdx2: caudal type homeobox 2; E2f1: E2 promoter binding factor 1; Atp5c1: adenosine triphosphate synthase subunit gamma; Sall1: spalt like transcription factor 1; Klf5: krüppel-like factor 5; Edn1: endothelin 1; Zfp382: zinc finger protein 382; Hmg20a: high mobility Group 20a; Bach2: BTB domain and CNC homolog 2; Bcl6b: B-cell Lymphoma 6b; Rnf2: ring finger protein 2; Cbx2: chromobox 2; Esr1: estrogen receptor 1; Shox2: short Stature homobox 2; Cav1: caveolin-1; Pias1: protein inhibitor of activated signal transducer and activators of transcription 1; Dcaf1: DDB1 and CUL4 associated factor 1. The difference between the two groups was measured by Student’s t-test. Data are shown as the mean?±?standard deviation (n = 5). aP < 0.05, compared with the control group. bP < 0.01 and cP < 0.05, compared with the model group.
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