Journal of Traditional Chinese Medicine ›› 2025, Vol. 45 ›› Issue (6): 1317-1329.DOI: 10.19852/j.cnki.jtcm.2025.06.011
• Original Articles • Previous Articles Next Articles
Psoriasis intervention by Huai’er (Trametes): unveiling novel targets via network pharmacology
LI Keyao1, SHU Ye2, CHANG Jing2, TANG Jianping2, ZHANG Litao3(
), WEI Zhu4()
- 1 Department of Dermatology, Hunan Children’s Hospital, Children’s Hospital Affiliated to Xiangya Medical College of Central South University, Changsha 410007, China; Graduate School, Tianjin Medical University, Tianjin 300070, China
2 Department of Dermatology, Hunan Children’s Hospital, Children’s Hospital Affiliated to Xiangya Medical College of Central South University, Changsha 410007, China
3 Department of Dermatology, Tianjin Medical University, Tianjin 300070, China; Affiliated Hospital of Tianjin Academy of Traditional Chinese Medicine, Tianjin 300193, China
4 Department of Dermatology, Hunan Children’s Hospital, Children’s Hospital Affiliated to Xiangya Medical College of Central South University, Changsha 410007, China
-
Received:2025-09-12Accepted:2025-01-26Online:2025-12-15Published:2025-11-24 -
Contact:ZHANG Litao, Tianjin Medical University, Affiliated Hospital of Tianjin Academy of Traditional Chinese Medicine, Tianjin 300193, China. zhanglitao@medmail.com.cn, Telephone: +86-13467622617;
WEI Zhu, Department of Dermatology, Hunan Children's Hospital, Children's Hospital Affiliated to Xiangya Medical College of Central South University, Changsha 410007, China. 34438881@qq.com -
Supported by:Scientific Research Project of Hunan Provincial Health Commission: Effects and Mechanisms of Huaiqi Huang on the Proliferation and Migration of Psoriasis HaCaT Cells(D202304128233)
Cite this article
LI Keyao, SHU Ye, CHANG Jing, TANG Jianping, ZHANG Litao, WEI Zhu. Psoriasis intervention by Huai’er (Trametes): unveiling novel targets via network pharmacology[J]. Journal of Traditional Chinese Medicine, 2025, 45(6): 1317-1329.
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Figure 1 Investigation of the effects of Huai'er (Trametes) on HaCaT cell behavior A: bright-field/phase-contrast image of HaCaT cells without staining. Scale bar = 50 μm; B: CCK-8 cell viability of HaCaT cells treated with Huai'er (Trametes) at 0, 2, 4, 8, and 16 mg/mL for 24, 48, and 72 h. Two-way ANOVA (factors: concentration and time) was performed with post-hoc tests when appropriate; C: EdU fluorescence staining of HaCaT cells treated with 8 mg/mL Huai'er (Trametes) for different time points.C1: 0 h; C2: 12 h; C3: 24 h; C4: 48 h. EdU? cells are shown in red; nuclei are stained with DAPI (blue). Scale bar = 50 μm.); D: percentage of EdU? cells in different time groups (0, 12, 24, 48 h). AEdU: 5-ethynyl-2′-deoxyuridine; DAPI: 4′, 6-diamidino-2-phenylindole; CCK-8: cell counting kit-8; ANOVA: analysis of variance. Data are presented as mean ± standard deviation (n = 3). One-way ANOVA was used for statistical analysis. Compared with the 0 h group, aP < 0.01.
Figure 1 Investigation of the effects of Huai'er (Trametes) on HaCaT cell behavior A: bright-field/phase-contrast image of HaCaT cells without staining. Scale bar = 50 μm; B: CCK-8 cell viability of HaCaT cells treated with Huai'er (Trametes) at 0, 2, 4, 8, and 16 mg/mL for 24, 48, and 72 h. Two-way ANOVA (factors: concentration and time) was performed with post-hoc tests when appropriate; C: EdU fluorescence staining of HaCaT cells treated with 8 mg/mL Huai'er (Trametes) for different time points.C1: 0 h; C2: 12 h; C3: 24 h; C4: 48 h. EdU? cells are shown in red; nuclei are stained with DAPI (blue). Scale bar = 50 μm.); D: percentage of EdU? cells in different time groups (0, 12, 24, 48 h). AEdU: 5-ethynyl-2′-deoxyuridine; DAPI: 4′, 6-diamidino-2-phenylindole; CCK-8: cell counting kit-8; ANOVA: analysis of variance. Data are presented as mean ± standard deviation (n = 3). One-way ANOVA was used for statistical analysis. Compared with the 0 h group, aP < 0.01.
Figure 2 Investigation of the effects of Huai'er (Trametes) on HaCaT cell behavior and expression of inflammatory factors A: scratch-wound assay of HaCaT cells imaged by phase-contrast microscopy (no staining); a 100 μm scale bar is shown at the bottom right of each image. A1-A4: representative images of Control and Huai'er (Trametes) at 0 h and 24 h (A1: Control group at 0 h; A2: Huai’er (Trametes) group at 0 h; A3: Control group at 24 h; A4: Huai’er (Trametes) group at 24 h); A5: quantification of migration distance (%); B: RT-qPCR analysis of inflammatory cytokine mRNA levels. B1: IL-6; B2: IL-8; B3: IL-10; B4: IL-23; B5: IL-17; B6: TNF-α. The cell experiments mentioned above were repeated three times. Control: untreated; Huai'er (Trametes): treated with Huai'er (Trametes) at 8 mg/mL for 24 h; scratch assay time points: 0 h and 24 h. RT-qPCR: reverse transcription quantitative polymerase chain reaction; IL: interleukin; TNF-α: tumor necrosis factor alpha; ANOVA: analysis of variance. Data are presented as mean ± standard deviation (n = 3). Two-group comparisons used a two-tailed unpaired t-test (or one-way ANOVA with post-hoc tests, keep consistent with your methods). Compared to the Control group, aP < 0.01.
Figure 2 Investigation of the effects of Huai'er (Trametes) on HaCaT cell behavior and expression of inflammatory factors A: scratch-wound assay of HaCaT cells imaged by phase-contrast microscopy (no staining); a 100 μm scale bar is shown at the bottom right of each image. A1-A4: representative images of Control and Huai'er (Trametes) at 0 h and 24 h (A1: Control group at 0 h; A2: Huai’er (Trametes) group at 0 h; A3: Control group at 24 h; A4: Huai’er (Trametes) group at 24 h); A5: quantification of migration distance (%); B: RT-qPCR analysis of inflammatory cytokine mRNA levels. B1: IL-6; B2: IL-8; B3: IL-10; B4: IL-23; B5: IL-17; B6: TNF-α. The cell experiments mentioned above were repeated three times. Control: untreated; Huai'er (Trametes): treated with Huai'er (Trametes) at 8 mg/mL for 24 h; scratch assay time points: 0 h and 24 h. RT-qPCR: reverse transcription quantitative polymerase chain reaction; IL: interleukin; TNF-α: tumor necrosis factor alpha; ANOVA: analysis of variance. Data are presented as mean ± standard deviation (n = 3). Two-group comparisons used a two-tailed unpaired t-test (or one-way ANOVA with post-hoc tests, keep consistent with your methods). Compared to the Control group, aP < 0.01.
Figure 3 Network pharmacology investigation of potential targets and mechanisms of Huai'er (Trametes) in treating psoriasis
Figure 3 Network pharmacology investigation of potential targets and mechanisms of Huai'er (Trametes) in treating psoriasis
Figure 4 Network pharmacology investigation of potential targets and mechanisms of Huai'er (Trametes) in treating psoriasis
Figure 4 Network pharmacology investigation of potential targets and mechanisms of Huai'er (Trametes) in treating psoriasis
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