Journal of Traditional Chinese Medicine ›› 2022, Vol. 42 ›› Issue (6): 980-987.DOI: 10.19852/j.cnki.jtcm.20220817.001
• Research Articles • Previous Articles Next Articles
Efficacy of Moxa-burning heat stimulating Zusanli (ST36) and Shenshu (BL23) on expressions of macrophage migration inhibitory factor and macrophage apoptosis in rabbits with adjuvant-induced arthritis
ZHOU Haiyan, ZHONG Yumei2, GAO Xiuhua3, WU Fei4, JIA Min5(
), YANG Xin6()
- 1 School of Acupuncture-Moxibustion and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China
2 Pain Department, Chengdu First People's Hospital/ Chengdu Integrated TCM & Western Medicine Hospital, Chengdu 610095, China
3 Centre of Preventive Treatment of Disease, Teaching Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China
4 Foreign Languages School, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China
5 Acupuncture Department, Second Affiliated Hospital of Baotou Medical College, Baotou 014030, China
6 Health Rehabilitation School, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China
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Received:2021-09-16Accepted:2021-12-21Online:2022-12-15Published:2022-08-17 -
Contact:JIA Min,YANG Xin -
About author:Associate Prof. YANG Xin, Health Rehabilitation School, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China. yangxin@cdutcm.edu.cn,Telephone: +86-13551039390
Associate chief doctor JIA Min, Acupuncture Department, Second Affiliated Hospital of Baotou Medical College, Baotou 014030, China. 401877133@qq.com;
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Supported by:Study on the Immune Mechanisms of Macrophage M1/M2 Polarization in the Treatment of Rheumatoid Arthritis by Moxibustion “Strengthening Body Resistance and Eliminating Evil”(81973959);Research on “Immune-Inflammation” Molecular Signal Regulation of NLRP3 Inflammasomes in RA with Moxibustion Treatment(81774435);Research on the Functional Characterics of “Special Effect” and “Common Effect” of Acupoints(2019YFC1709001);Research on MIF-GC Rhythm Regulation Mechanism of Moxibustion in the Treatment of Rheumatoid Arthritis(2018JC007);Research on the Mechanism of “MIF-target Protein-GC-inflammation” of Anti-inflammatory Effect of Moxibustion on Rheumatoid Arthritis(QNXZ2018034)
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ZHOU Haiyan, ZHONG Yumei, GAO Xiuhua, WU Fei, JIA Min, YANG Xin. Efficacy of Moxa-burning heat stimulating Zusanli (ST36) and Shenshu (BL23) on expressions of macrophage migration inhibitory factor and macrophage apoptosis in rabbits with adjuvant-induced arthritis[J]. Journal of Traditional Chinese Medicine, 2022, 42(6): 980-987.
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Figure 1 Apoptosis rate of macrophages in control, RA and Moxibustion group A-C: terminal deoxynucleotide transferase-mediated dUTP nick end labeling staining (× 400). A to C representative images show the apoptosis of macrophages in control (A), RA (B) and moxibustion (C) group. Black arrow indicates the apoptosis of macrophages. D showed the statistical analysis of the apoptosis of macrophages. n =10 for each group. RA group and Moxa group were established by injecting FCA (0.5 mL/kg) into the bilateral posterior knee joints. Control group was injected with the same volume sterile normal saline in the same way. Moxa group was treated by moxa-burning heat on Zusanli (ST36) and Shenshu (BL23) once 1 d for 3 courses after FCA injection. Other two groups were fixed with the same method for the same time without moxibustion. Data were expressed as the mean ± standard deviation. Compared with the Control group, aP < 0.01; compared with the RA group, bP < 0.05. RA: rheumatoid arthritis; FCA: Freund’s complete adjuvant.
Figure 1 Apoptosis rate of macrophages in control, RA and Moxibustion group A-C: terminal deoxynucleotide transferase-mediated dUTP nick end labeling staining (× 400). A to C representative images show the apoptosis of macrophages in control (A), RA (B) and moxibustion (C) group. Black arrow indicates the apoptosis of macrophages. D showed the statistical analysis of the apoptosis of macrophages. n =10 for each group. RA group and Moxa group were established by injecting FCA (0.5 mL/kg) into the bilateral posterior knee joints. Control group was injected with the same volume sterile normal saline in the same way. Moxa group was treated by moxa-burning heat on Zusanli (ST36) and Shenshu (BL23) once 1 d for 3 courses after FCA injection. Other two groups were fixed with the same method for the same time without moxibustion. Data were expressed as the mean ± standard deviation. Compared with the Control group, aP < 0.01; compared with the RA group, bP < 0.05. RA: rheumatoid arthritis; FCA: Freund’s complete adjuvant.
Figure 2 Immunohistochemical assessment the expression of ERK2 in control, RA, and moxibustion group A-C: immunohistochemistry (S-P) analysis (×400). Representative images show the expression of ERK2 in synovial tissues of control (A), RA (B) and moxibustion (C) group. Black arrow indicates the position expression of ERK2. D represented the statistical analysis of the expression of ERK2. n =10 for each group. RA group and Moxa group were established by injecting FCA (0.5 mL/kg) into the bilateral posterior knee joints. Control group was injected with the same volume sterile normal saline in the same way. Moxa group was treated by moxa-burning heat on Zusanli (ST36) and Shenshu (BL23) once 1day for 3 courses after FCA injection. Other two groups were fixed with the same method for the same time without moxibustion. Data were expressed as the mean ± standard deviation. Compared with the Control group, aP < 0.01; compared with the RA group, bP < 0.05. ERK: extracellular regulated protein kinases; RA: rheumatoid arthritis; FCA: Freund’s complete adjuvant.
Figure 2 Immunohistochemical assessment the expression of ERK2 in control, RA, and moxibustion group A-C: immunohistochemistry (S-P) analysis (×400). Representative images show the expression of ERK2 in synovial tissues of control (A), RA (B) and moxibustion (C) group. Black arrow indicates the position expression of ERK2. D represented the statistical analysis of the expression of ERK2. n =10 for each group. RA group and Moxa group were established by injecting FCA (0.5 mL/kg) into the bilateral posterior knee joints. Control group was injected with the same volume sterile normal saline in the same way. Moxa group was treated by moxa-burning heat on Zusanli (ST36) and Shenshu (BL23) once 1day for 3 courses after FCA injection. Other two groups were fixed with the same method for the same time without moxibustion. Data were expressed as the mean ± standard deviation. Compared with the Control group, aP < 0.01; compared with the RA group, bP < 0.05. ERK: extracellular regulated protein kinases; RA: rheumatoid arthritis; FCA: Freund’s complete adjuvant.
Figure 3 Immunohistochemical assessment the expression of p38MAPK in control, RA and Moxa group A-C: immunohistochemistry (S-P) analysis (× 400). A to C representative images show the expression of p38MAPK in synovial tissues of control (A), RA (B) and moxibustion (C) group. D represented the statistical analysis of the expression of p38MAPK. n =10 for each group. RA group and moxa group were established by injecting FCA (0.5 mL/kg) into the bilateral posterior knee joints. Control group was injected with the same volume sterile normal saline in the same way. Moxa group was treated by moxa-burning heat on Zusanli (ST36) and Shenshu (BL23) once 1 d for 3 courses after FCA injection. Other two groups were fixed with the same method for the same time without moxibustion. Data were expressed as the mean ± standard deviation. Compared with the control group, aP < 0.01; compared with RA group, bP < 0.05. MAPK: mitogen-activated protein kinase; RA: rheumatoid arthritis; FCA: Freund’s complete adjuvant.
Figure 3 Immunohistochemical assessment the expression of p38MAPK in control, RA and Moxa group A-C: immunohistochemistry (S-P) analysis (× 400). A to C representative images show the expression of p38MAPK in synovial tissues of control (A), RA (B) and moxibustion (C) group. D represented the statistical analysis of the expression of p38MAPK. n =10 for each group. RA group and moxa group were established by injecting FCA (0.5 mL/kg) into the bilateral posterior knee joints. Control group was injected with the same volume sterile normal saline in the same way. Moxa group was treated by moxa-burning heat on Zusanli (ST36) and Shenshu (BL23) once 1 d for 3 courses after FCA injection. Other two groups were fixed with the same method for the same time without moxibustion. Data were expressed as the mean ± standard deviation. Compared with the control group, aP < 0.01; compared with RA group, bP < 0.05. MAPK: mitogen-activated protein kinase; RA: rheumatoid arthritis; FCA: Freund’s complete adjuvant.
Figure 4 Immunohistochemical assessment the expression of NF-κBp65 in control, RA, and moxibustion group A-C: Immunohistochemistry (S-P) analysis (× 400). A to C representative images show the expression of NF-κBp65 in synovial tissues of control (A), RA (B) and moxibustion (C) group. Black arrow indicates the position expression of NF-κBp65. D represented the statistical analysis of the expression of NF-κBp65. n =10 for each group. RA group and Moxa group were established by injecting FCA (0.5 mL/kg) into the bilateral posterior knee joints. Control group was injected with the same volume sterile normal saline in the same way. Moxa group was treated by moxa-burning heat on Zusanli (ST36) and Shenshu (BL23) once 1day for 3 courses after FCA injection. Other two groups were fixed with the same method for the same time without moxibustion. Data were expressed as the mean ± standard deviation. Compared with the Control group, aP < 0.05; compared with the RA group, bP < 0.05. NF-κB: nuclear factor-κ-gene binding; RA: rheumatoid arthritis; FCA: Freund’s complete adjuvant.
Figure 4 Immunohistochemical assessment the expression of NF-κBp65 in control, RA, and moxibustion group A-C: Immunohistochemistry (S-P) analysis (× 400). A to C representative images show the expression of NF-κBp65 in synovial tissues of control (A), RA (B) and moxibustion (C) group. Black arrow indicates the position expression of NF-κBp65. D represented the statistical analysis of the expression of NF-κBp65. n =10 for each group. RA group and Moxa group were established by injecting FCA (0.5 mL/kg) into the bilateral posterior knee joints. Control group was injected with the same volume sterile normal saline in the same way. Moxa group was treated by moxa-burning heat on Zusanli (ST36) and Shenshu (BL23) once 1day for 3 courses after FCA injection. Other two groups were fixed with the same method for the same time without moxibustion. Data were expressed as the mean ± standard deviation. Compared with the Control group, aP < 0.05; compared with the RA group, bP < 0.05. NF-κB: nuclear factor-κ-gene binding; RA: rheumatoid arthritis; FCA: Freund’s complete adjuvant.
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