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Journal of Traditional Chinese Medicine ›› 2021, Vol. 41 ›› Issue (6): 943-952.DOI: 10.19852/j.cnki.jtcm.2021.06.011

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Direct contact moxibustion promotes apoptosis of gastric cancer cells in rats by regulating intestinal flora

PAN Lijia, MA Shuya, WEN Jing, ZHANG Xiaoqi, XING Haijiao, JIA Chunsheng   

  1. College of Acupuncture and Moxibustion, Hebei University of Chinese Medicine;
  • Online:2021-12-07 Published:2021-12-07
  • Supported by:
    Supported by the National Natural Science Fund:the Biophysical Properties Research of Related Acupoints Based on the Physiological State of Uterus (No. 81603542), Study on Advantages of Disease and the Law of Micro-Needle Therapy by Using Data Mining Technology (No. 81473773), the Project of Improving Scientific Research Ability of Hebei University of Chinese Medicine:the Immune Initiation Effect of Dendritic Cells on Rat with Gastric Cancer Treating by Direct Moxibustion (No. KTZ2019012), Postgraduate Innovation Project of Hebei University of Chinese Medicine:the Immune Initiation Effect of Dendritic Cells on Rat with Gastric Cancer Treating by Direct Moxibustion (No. XCXZZBS2020012)

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Abstract: OBJECTIVE:To examine whether direct contact moxibustion(DCM) can prevent and treat gastric cancer(GC) by regulating intestinal flora in rats.METHODS:Male Wistar rats were randomly divided into normal group, normal+DCM control group, model group, and model+DCM group. Gastric cancer rats were induced by N-methyl-N-nitro-N-nitrosoguanidine(MNNG, 20 mg/mL) by gavage. At the same time, the model rats and normal rats were given DCM at Zusanli(ST36), Weishu(BL21), and Zhongwan(CV12) for 16 weeks. After treatment, gastric tissues were collected to analyze the pathological changes and the apoptosis of gastric mucosa cells. In addition, the cecal stool was taken and analyzed by 16 s r RNA sequencing.RESULTS:Gastric cancer-like pathological changes and different abundance of the intestinal flora were found in the model group. DCM promoted mucosa tissue apoptosis and regulated the abnormal changes of the intestinal microflora caused by MNNG; DCM also inhibited the growth of Ruminococcaceae and Prevotellaceae flora and promoted the growth of probiotic Akkermansia. Furthermore,DCM made the composition and abundance of intestinal microflora in the GC rats tending to the normal rats.CONCLUSION:DCM stimulating Zusanli(ST36),Weishu(BL21), and Zhongwan(CV12) promoted the apoptosis of gastric mucosa and delayed the progression of gastric cancer, possibly by decreasing Ruminococcaceae and Prevotellaceae bacteria(bacteria that produce short-chain fatty acids in the intestine) and promoting the growth of probiotic Akkermansia.

Key words: moxibustion, methylnitronitrosoguanidine, stomach neoplasms, gastrointestinal microbiome

Cite this article

PAN Lijia, MA Shuya, WEN Jing, ZHANG Xiaoqi, XING Haijiao, JIA Chunsheng. Direct contact moxibustion promotes apoptosis of gastric cancer cells in rats by regulating intestinal flora[J]. Journal of Traditional Chinese Medicine, 2021, 41(6): 943-952.