Journal of Traditional Chinese Medicine ›› 2024, Vol. 44 ›› Issue (4): 753-761.DOI: 10.19852/j.cnki.jtcm.20240610.003
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GUO Yanding1, LUO Kun1, ZHANG Linlin1, LU Wenting3, SHANG Yanan1, ZHONG Yumei2, HU Danhui1, YANG Xin4, ZHOU Haiyan1()
Received:
2023-02-12
Accepted:
2023-07-20
Online:
2024-08-15
Published:
2024-06-10
Contact:
ZHOU Haiyan, Acupuncture and Moxibustion College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China. Supported by:
GUO Yanding, LUO Kun, ZHANG Linlin, LU Wenting, SHANG Yanan, ZHONG Yumei, HU Danhui, YANG Xin, ZHOU Haiyan. Study on the anti-inflammatory mechanism of moxibustion in rheumatoid arthritis in rats based on phospholipaseA2 signaling inhibition by Annexin 1[J]. Journal of Traditional Chinese Medicine, 2024, 44(4): 753-761.
Pathological changes score | 0 | 1 | 2 | 3 |
---|---|---|---|---|
Synovial tissue hyperplasia | None (-) | Mild (+) | Moderate (++) | Massive (+++) |
Inflammatory cell infitration | None (-) | Few (+) | Dense (++) | Numerous (+++) |
Macrophage proliferation | None (-) | Few (+) | Dense (++) | Numerous (+++) |
Table 1 Classification criteria of synovial tissue lesions
Pathological changes score | 0 | 1 | 2 | 3 |
---|---|---|---|---|
Synovial tissue hyperplasia | None (-) | Mild (+) | Moderate (++) | Massive (+++) |
Inflammatory cell infitration | None (-) | Few (+) | Dense (++) | Numerous (+++) |
Macrophage proliferation | None (-) | Few (+) | Dense (++) | Numerous (+++) |
Figure 1 Effect of moxibustion on reducing the swelling of the foot A: photos of the control group. A1: photo of the rat foot pad; A2: HE stained image of ankle synovial tissue (× 20); A3: HE staining magnified image of ankle joint synovial tissue (× 200); B: photos of the rheumatoid arthritis group. B1: photo of the rat foot pad; B2: HE stained image of ankle synovial tissue (× 20); B3: HE staining magnified image of ankle joint synovial tissue (× 200); C: photos of the moxibustion group. C1: photo of the rat foot pad; C2: HE stained image of ankle synovial tissue (× 20); C3: HE staining magnified image of ankle joint synovial tissue (× 200); D: line diagram of the thickness change of the left foot pad in each group after three cycles of moxibustion treatment. E: line diagram of the thickness change of the right foot pad in each group after three cycles of moxibustion treatment. The control group received 0.1 mL of saline with bilateral foot pads; the rheumatoid arthritis group received 0.1 mL of FCA with bilateral foot pads; the moxibustion group received 0.1 mL of FCA with bilateral foot pads for three cycles of moxibustion treatment. HE: hematoxylin-eosin staining; FCA: Freund's Adjuvant Complete. The Data represent the mean ± standard deviation using one-way analysis of variance (n = 6). aP < 0.01 versus the control group; bP < 0.01 versus the rheumatoid arthritis group.
Figure 2 Moxibustion regulated the expression of inflammatory factors in the serum A: serum IL-1β level in rats from each group; B: serum IL-10 level in rats from each group; C: serum PGE2 levels in rats from each group; D: serum LTB4 level in rats from each group. The rheumatoid arthritis and moxibustion groups injected 0.1 mL of FCA, the control group received an equal amount of normal saline, and three cycles of moxibustion operation for the moxibustion group. IL-1β: interleukin-1beta; IL-10: interleukin-10; PGE2: pmstaglandi E2; LTB4: leukotrine4; FCA: Freund's Adjuvant Complete. The Data represent the mean ± standard deviation using one-way analysis of variance (n = 6). aP < 0.01, compared with the control group; bP < 0.01, compared with the rheumatoid arthritis group.
Figure 3 Measurement of Annexin 1, cPLA2α and p-cPLA2α expression in synovium by Weston blot A: Weston blot was used to detect the expression of Annexin 1, cPLA2α and p-cPLA2α in the synovium of rats ankle joint. B: Western blot analysis of Annexin 1; C: Western blot analysis of cPLA2α; D: Western blot analysis of p-cPLA2α. The rheumatoid arthritis, moxibustion, Annexin 1 lentiviral intervention and Annexin 1 lentiviral intervention + moxibustion intervention groups injected 0.1 mL of FCA, the control group received an equal amount of normal saline, and three cycles of moxibustion operation for the moxibustion and Annexin 1 lentiviral intervention + moxibustion intervention groups. Lentiviral vector-mediated RNAi-Anxa 1 was injected at a dose of 10 μL / rat into the rat foot pads of the Annexin 1 lentiviral intervention and Annexin 1 lentiviral intervention + moxibustion intervention groups. cPLA2α: phospholipaseA2; p-cPLA2α: phosphor-phospholipaseA2; FCA: Freund's Adjuvant Complete. The Data represent the mean ± standard deviation using one-way analysis of variance (n = 6). aP < 0.01, compared with the control group; bP < 0.01, compared with the rheumatoid arthritis group, cP < 0.01, compared with the moxibustion group.
Figure 4 Moxibustion treatment in RA rats can regulate downstream inflammatory cytokines and exert anti-inflammatory effects via Annexin 1/cPLA2α A: serum IL-10 level in rats from each group; B: serum IL-1β level in rats from each group; C: serum LTB4 level in rats from each group; D: serum PGE2 level in rats from each group. The rheumatoid arthritis, moxibustion, Annexin 1 lentiviral intervention and Annexin 1 lentiviral intervention + moxibustion intervention groups injected 0.1 mL of FCA, the control group received an equal amount of normal saline, and three cycles of moxibustion operation for the moxibustion and Annexin 1 lentiviral intervention + moxibustion intervention groups. Lentiviral vector-mediated RNAi-Anxa 1 was injected at a dose of 10 μL/rat into the rat foot pads of the Annexin 1 lentiviral intervention and Annexin 1 lentiviral intervention + moxibustion intervention groups. IL-10: interleukin- 10; IL-1β: interleukin- 1beta; PGE2: pmstaglandi E2; LTB4: leukotrine4; FCA: Freund's adjuvant complete. The Data represent the mean ± standard deviation using one-way analysis of variance (n = 6). aP < 0.01, compared with the control group; bP < 0.01, compared with the rheumatoid arthritis group, cP < 0.01, compared with the moxibustion group.
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