Journal of Traditional Chinese Medicine ›› 2024, Vol. 44 ›› Issue (3): 515-523.DOI: 10.19852/j.cnki.jtcm.20240423.006
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WANG Xiang1, HUANG Jianping1, WANG Yupeng3, WANG Qilong1, JING Yajiang1, ZHANG Gang1, PENG Liang1, GAO Jing1, WANG Hongyan2(), YAN Yonggang1()
Received:
2023-02-22
Accepted:
2023-07-14
Online:
2024-06-15
Published:
2024-04-30
Contact:
WANG Hongyan,YAN Yonggang
About author:
Prof. YAN Yonggang, School of Pharmacy, Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, China. yunfeng828@163.com Telephone: +86-13759845952; +86-13892062171Supported by:
WANG Xiang, HUANG Jianping, WANG Yupeng, WANG Qilong, JING Yajiang, ZHANG Gang, PENG Liang, GAO Jing, WANG Hongyan, YAN Yonggang. Differential metabolite analysis of the pharmacodynamic differences between different ratios of Dahuang (Radix Et Rhizoma Rhei Palmati)-Taoren (Semen Persicae) herb pair[J]. Journal of Traditional Chinese Medicine, 2024, 44(3): 515-523.
Figure 1 PCA plots were compared between groups A: PCA analysis of groups A and B; B: PCA analysis of groups B and C; C: PCA analysis of groups A and C. DH and TR were ground and weighed proportionally in three groups: group A = 25 g: 25 g, B = 20 g: 30 g and C = 30 g: 20 g. Then each group of samples was separately decocted with water and the filtrate was concentrated to 1 g/mL to obtain. PCA: principal component analysis; DH: Dahuang (Radix et Rhizoma Rhei Palnati); TR: Taoren (Semen Persicae). The PC value represents the proportion of principal components.
Number | Metabolite name | Number | Metabolite name |
---|---|---|---|
Com_14 | 2,3-dihydroxypyridine | Com_55 | 2-deoxy-D-glucose 2 |
Com_17 | (2R,3S)-2-hydroxy-3-isopropylbutanedioic acid | Com_89 | Asparagine 1 |
Com_52 | Raffinose | Com_91 | Asparagine 4 |
Com_118 | Oleic acid | Com_92 | Malonamide 3 |
Com_178 | Melezitose | Com_98 | Glutamic acid |
Com_38 | Glucose-1-phosphate | Com_113 | Galactinol 1 |
Com_46 | Palatinitol 1 | Com_120 | Glycine 2 |
Com_49 | Salicin | Com_135 | Quinic acid |
Com_53 | 4,2',4'-trihydroxychalcone | Com_138 | Naringin |
Com_110 | Putrescine 2 | Com_140 | Aminomalonic acid |
Com_132 | Gentiobiose 1 | Com_146 | L-dithiothreitol |
Com_151 | Prunin degr Prod 2 | Com_152 | 3-cyanoalanine |
Com_158 | Melibiose 1 | Com_157 | Orotic acid |
Com_199 | Epigallocatechin | Com_160 | Tryptophan 1 |
Com_201 | Levoglucosan | Com_163 | Benzyl alcohol |
Com_206 | (+/-)-Taxifolin | Com_187 | Sucrose-6-phosphate |
Com_216 | Chlorogenic acid 1 | Com_203 | Serine 1 |
Com_219 | Noradrenaline | Com_205 | Aspartic acid 1 |
Com_6 | Allose 1 | Com_215 | 4-Acetamidobutyric acid 2 |
Com_12 | Toluenesulfonic acid | Com_229 | Leucrose 2 |
Com_18 | Diglycerol 1 |
Table 1 Differential metabolites
Number | Metabolite name | Number | Metabolite name |
---|---|---|---|
Com_14 | 2,3-dihydroxypyridine | Com_55 | 2-deoxy-D-glucose 2 |
Com_17 | (2R,3S)-2-hydroxy-3-isopropylbutanedioic acid | Com_89 | Asparagine 1 |
Com_52 | Raffinose | Com_91 | Asparagine 4 |
Com_118 | Oleic acid | Com_92 | Malonamide 3 |
Com_178 | Melezitose | Com_98 | Glutamic acid |
Com_38 | Glucose-1-phosphate | Com_113 | Galactinol 1 |
Com_46 | Palatinitol 1 | Com_120 | Glycine 2 |
Com_49 | Salicin | Com_135 | Quinic acid |
Com_53 | 4,2',4'-trihydroxychalcone | Com_138 | Naringin |
Com_110 | Putrescine 2 | Com_140 | Aminomalonic acid |
Com_132 | Gentiobiose 1 | Com_146 | L-dithiothreitol |
Com_151 | Prunin degr Prod 2 | Com_152 | 3-cyanoalanine |
Com_158 | Melibiose 1 | Com_157 | Orotic acid |
Com_199 | Epigallocatechin | Com_160 | Tryptophan 1 |
Com_201 | Levoglucosan | Com_163 | Benzyl alcohol |
Com_206 | (+/-)-Taxifolin | Com_187 | Sucrose-6-phosphate |
Com_216 | Chlorogenic acid 1 | Com_203 | Serine 1 |
Com_219 | Noradrenaline | Com_205 | Aspartic acid 1 |
Com_6 | Allose 1 | Com_215 | 4-Acetamidobutyric acid 2 |
Com_12 | Toluenesulfonic acid | Com_229 | Leucrose 2 |
Com_18 | Diglycerol 1 |
Figure 3 Correlation analysis of different metabolites in each group A: correlation analysis of different metabolites in group A and B; B: correlation analysis of different metabolites in group B and C; C: correlation analysis of different metabolites in group A and C. DH and TR were ground and weighed proportionally in three groups: group A = 25 g: 25 g, B = 20 g: 30 g and C = 30 g: 20 g. Then each group of samples was separately decocted with water and the filtrate was concentrated to 1 g/mL to obtain. DH: Dahuang (Radix et Rhizoma Rhei Palnati); TR: Taoren (Semen Persicae). The complete positive correlation is red, and the complete negative correlation is blue. The part without color indicates P > 0.05.
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