Journal of Traditional Chinese Medicine ›› 2023, Vol. 43 ›› Issue (5): 1026-1033.DOI: 10.19852/j.cnki.jtcm.2023.05.006
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Xiaoyi Yusi decoction (消异育嗣汤) improves in vitro fertilization and embryo transfer outcomes in patients with endometriosis
JIANG Wenjing1, JIANG Huaying2,3, YUAN Lihua4, SA Yuanhong5, XIAO Jimei6, SUN Hongqi7, SONG Jingyan8,9(
), SUN Zhengao8,9()
- 1 First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan 250014, China
2 Emergency Department, Laizhou Maternal and Child Health Hospital of Shandong, Laizhou 261400, China
3 College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250014, China
4 Reproductive and Genetic Center of Integrated Traditional and Western Medicine, the Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China
5 Department of Traditional Chinese Medicine, Qingyun County Maternity and Child Health Care Hospital of Dezhou, Qingyun 253799, China
6 Reproductive Center of Heze Hospital of Traditional Chinese Medicine, Heze 274000, China
7 Zhengzhou Key Laboratory of Children's Infection and Immunity, Children's Hospital Affiliated to Zhengzhou University, Zhengzhou 450000, China
8 Reproductive and Genetic Center of Integrated Traditional and Western Medicine, the Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China
9 College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250014, China
-
Received:2022-05-23Accepted:2022-08-12Online:2023-10-15Published:2023-08-29 -
Contact:Prof. SONG Jingyan, Reproductive Medicine Center of Integration of Traditional and Western Medicine, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China.hanlingjuzei91@126.com ; Prof. SUN Zhengao, Reproductive Medicine Center of Integration of Traditional and Western Medicine, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China.sunzhengao77@126.com . Telephone: +86-531-68901236 -
Supported by:National Natural Science Fund Project: A Theoretical Study of Renal Reproduction Based on in vitro Fertilization Follicular Fluid Differential Protein-metabolite Global Regulatory Network(81874484)
Cite this article
JIANG Wenjing, JIANG Huaying, YUAN Lihua, SA Yuanhong, XIAO Jimei, SUN Hongqi, SONG Jingyan, SUN Zhengao. Xiaoyi Yusi decoction (消异育嗣汤) improves in vitro fertilization and embryo transfer outcomes in patients with endometriosis[J]. Journal of Traditional Chinese Medicine, 2023, 43(5): 1026-1033.
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Table 1 Clinical background of subjects
| Item | Patients group (n = 28) | Treatment group (n = 32) | Control group (n = 33) |
|---|---|---|---|
| Age (years) | 30.3±4.1 | 30.8±3.5 | 29.6±3.6 |
| Infertility years (years) | 3.4±1.9 | 3.1±2.0 | 2.2±1.5 |
| Primary infertility (n) | 17 | 16 | 15 |
| Secondary infertility (n) | 11 | 16 | 18 |
| Basic FSH (mIU/mL) | 7.4±3.8 | 7.5±3.3 | 7.0±2.5 |
| Basic LH (mIU/mL) | 4.6±1.7 | 4.9±2.7 | 4.9±1.7 |
| Basic E2 (pg/mL) | 48.5±29.2 | 49.9±26.8 | 45.5±27.5 |
Table 1 Clinical background of subjects
| Item | Patients group (n = 28) | Treatment group (n = 32) | Control group (n = 33) |
|---|---|---|---|
| Age (years) | 30.3±4.1 | 30.8±3.5 | 29.6±3.6 |
| Infertility years (years) | 3.4±1.9 | 3.1±2.0 | 2.2±1.5 |
| Primary infertility (n) | 17 | 16 | 15 |
| Secondary infertility (n) | 11 | 16 | 18 |
| Basic FSH (mIU/mL) | 7.4±3.8 | 7.5±3.3 | 7.0±2.5 |
| Basic LH (mIU/mL) | 4.6±1.7 | 4.9±2.7 | 4.9±1.7 |
| Basic E2 (pg/mL) | 48.5±29.2 | 49.9±26.8 | 45.5±27.5 |
Table 2 Total integral of the patient group and the treatment group before and after the experiment
| Group | n | Total integral (before treatment) | Total integral (after treatment) | Integral difference | P value |
|---|---|---|---|---|---|
| Patients | 28 | 11.4±1.8 | 11.1±1.7 | 0.29±1.03 | > 0.05 |
| Treatment | 32 | 11.8±1.7 | 8.7±2.0a | 3.06±1.73a | < 0.01 |
Table 2 Total integral of the patient group and the treatment group before and after the experiment
| Group | n | Total integral (before treatment) | Total integral (after treatment) | Integral difference | P value |
|---|---|---|---|---|---|
| Patients | 28 | 11.4±1.8 | 11.1±1.7 | 0.29±1.03 | > 0.05 |
| Treatment | 32 | 11.8±1.7 | 8.7±2.0a | 3.06±1.73a | < 0.01 |
Table 3 The number of follicles and endocrine parameters during the day of HCG
| Item | Patients group (n = 28) | Treatment group (n = 32) | Control group (n = 33) | |
|---|---|---|---|---|
| Number of oocyte (n) | 8.9±6.5 | 12.1±6.6a | 14.1±5.0a | |
| Number of embryos (n) | 2.1±1.8 | 3.1±2.4a | 4.4±2.1a | |
| Rate of high-quality egg (%) | 45.4 | 65.0a | 62.2a | |
| Rate of high-quality embryos (%) | 33.3 | 51.2b | 51.3b | |
| Biochemical pregnancy rate (%) | 75.0 | 81.3 | 87.9 | |
| Clinical pregnancy rate (%) | 57.1 | 71.9a | 87.9a | |
Table 3 The number of follicles and endocrine parameters during the day of HCG
| Item | Patients group (n = 28) | Treatment group (n = 32) | Control group (n = 33) | |
|---|---|---|---|---|
| Number of oocyte (n) | 8.9±6.5 | 12.1±6.6a | 14.1±5.0a | |
| Number of embryos (n) | 2.1±1.8 | 3.1±2.4a | 4.4±2.1a | |
| Rate of high-quality egg (%) | 45.4 | 65.0a | 62.2a | |
| Rate of high-quality embryos (%) | 33.3 | 51.2b | 51.3b | |
| Biochemical pregnancy rate (%) | 75.0 | 81.3 | 87.9 | |
| Clinical pregnancy rate (%) | 57.1 | 71.9a | 87.9a | |
Figure 1 Partial least-squares discriminant analysis scores plot in follicular fluid metabolomics analysis
Figure 1 Partial least-squares discriminant analysis scores plot in follicular fluid metabolomics analysis
Figure 2 Heat map from the data of significantly differential metabolites The horizontal axis of the figure shows a dendrogram of the samples. The vertical axis of the figure shows a dendrogram of the metabolite differences.
Figure 2 Heat map from the data of significantly differential metabolites The horizontal axis of the figure shows a dendrogram of the samples. The vertical axis of the figure shows a dendrogram of the metabolite differences.
Figure 3 VIP scores of each potential biomarker to the discrimination between the patient group and the treatment group The contribution of every potential biomarker to the discrimination between the patient group and the treatment group were ranked as VIP scores. VIP: variable importance in projection.
Figure 3 VIP scores of each potential biomarker to the discrimination between the patient group and the treatment group The contribution of every potential biomarker to the discrimination between the patient group and the treatment group were ranked as VIP scores. VIP: variable importance in projection.
Figure 4 Metabolic pathway analysis of significantly changed metabolites between the patient group and the treatment group The results showed that pyruvate and glycerophospholipid metabolism pathways and the citrate cycle are closely related to endometriosis, indicating that they may be targetted in the treatment of endometriosis with XYYSD. XYYSD: Xiaoyi Yusi decoction.
Figure 4 Metabolic pathway analysis of significantly changed metabolites between the patient group and the treatment group The results showed that pyruvate and glycerophospholipid metabolism pathways and the citrate cycle are closely related to endometriosis, indicating that they may be targetted in the treatment of endometriosis with XYYSD. XYYSD: Xiaoyi Yusi decoction.
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