Journal of Traditional Chinese Medicine ›› 2024, Vol. 44 ›› Issue (2): 260-267.DOI: 10.19852/j.cnki.jtcm.20240203.003
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Anti-inflammatory mechanism of the non-volatile ingredients originated from Guanghuoxiang (Pogostemonis Herba) based on high performance liquid chromatography-heated electron spray ionization-high resolution mass spectroscope and cell metabolomics
JING Wenguang1, LIN Xiaoyu2, LI Chu2, ZHAO Xiaoliang3, CHENG Xianlong1, WANG Penglong2, WEI Feng1(
), MA Shuangcheng1()
- 1 Institute of Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 102629, China
2 School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China
3 Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment of Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China
-
Received:2023-04-22Accepted:2023-09-05Online:2024-04-15Published:2024-02-03 -
Contact:MA Shuangcheng, Institute of Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 102629, China.masc@nifdc.org.cn ; WEI Feng, Institute of Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 102629, China.weifeng@nifdc.org.cn Telephone: +86-10-53852020 -
Supported by:Institute of Chinese Medicine Discipline Construction Project of National Institutes for Food and Drug Control: Disciplinary Construction Program of Chinese Medicine Institute of NIFDC(1020050090116);Training Fund for Academic Leaders of NIFDC(2023X10);Program of State Drug Administraion-Key Laboratory of Quality Control of Chinese Medicinal Materials and Decoction Pieces(2022GSMPA-KL02)
Cite this article
JING Wenguang, LIN Xiaoyu, LI Chu, ZHAO Xiaoliang, CHENG Xianlong, WANG Penglong, WEI Feng, MA Shuangcheng. Anti-inflammatory mechanism of the non-volatile ingredients originated from Guanghuoxiang (Pogostemonis Herba) based on high performance liquid chromatography-heated electron spray ionization-high resolution mass spectroscope and cell metabolomics[J]. Journal of Traditional Chinese Medicine, 2024, 44(2): 260-267.
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Figure 1 Total ion flow diagram of standard substances and the non-volatile ingredients of patchouli A: total ion flow diagram of the standard; B: total ion flow diagram of the sample.
Figure 1 Total ion flow diagram of standard substances and the non-volatile ingredients of patchouli A: total ion flow diagram of the standard; B: total ion flow diagram of the sample.
Figure 2 LPS induced cell inflammatory model A: toxicity of the samples to RAW264.7; B: NO content of each sample; C: IL-6 content of each sample; D: TNF-α content of each sample. LPS: lipid polysaccharide. Control group: without treatment; Model group: treated with lipopolysaccharide; Sample group: treated with lipopolysaccharide and non-volatile ingredients of patchouli of 5, 7.5, 10 mg/L. IL-6: interleukin-6; TNF-α: tumor necrosis factor-α. Student’s-test was used for statistical significance (P < 0.05); data are presented as mean ± standard deviation (n = 3). Significant differences compared with control group were designated as aP < 0.001 and with model group as bP < 0.001.
Figure 2 LPS induced cell inflammatory model A: toxicity of the samples to RAW264.7; B: NO content of each sample; C: IL-6 content of each sample; D: TNF-α content of each sample. LPS: lipid polysaccharide. Control group: without treatment; Model group: treated with lipopolysaccharide; Sample group: treated with lipopolysaccharide and non-volatile ingredients of patchouli of 5, 7.5, 10 mg/L. IL-6: interleukin-6; TNF-α: tumor necrosis factor-α. Student’s-test was used for statistical significance (P < 0.05); data are presented as mean ± standard deviation (n = 3). Significant differences compared with control group were designated as aP < 0.001 and with model group as bP < 0.001.
Figure 3 Metabolomic difference analysis A: total ion flow diagram of each group; B: PCA scores; C: OPLS-DA scores of normal group, model group and treated group; D: OPLS-DA scores of normal group and model group; E: OPLS-DA scores of the treated group and model group; F: S-plot of normal group and model group; G: S-plot of the treated group and model group; Control group: without treatment; Model group: treated with lipopolysaccharide; Sample group: treated with lipopolysaccharide and non-volatile ingredients of patchouli of 10 mg/L. PCA: principal component analysis; OPLS-DA: orthogonal partial least squares discriminant analysis. Student’s t-test was used for statistical significance (P < 0.05); Data are presented as mean ± standard deviation (n = 3).
Figure 3 Metabolomic difference analysis A: total ion flow diagram of each group; B: PCA scores; C: OPLS-DA scores of normal group, model group and treated group; D: OPLS-DA scores of normal group and model group; E: OPLS-DA scores of the treated group and model group; F: S-plot of normal group and model group; G: S-plot of the treated group and model group; Control group: without treatment; Model group: treated with lipopolysaccharide; Sample group: treated with lipopolysaccharide and non-volatile ingredients of patchouli of 10 mg/L. PCA: principal component analysis; OPLS-DA: orthogonal partial least squares discriminant analysis. Student’s t-test was used for statistical significance (P < 0.05); Data are presented as mean ± standard deviation (n = 3).
Figure 4 Metabolomics pathway analysis A: clustering heat map of differential metabolites in the normal group and the model group; B: clustering heat map of differential metabolites in the treated group and the model group; C: arginine levels in cell samples; D: sorbitol levels in cell samples; E: KEGG enrichment pathway map of differential metabolic pathway between the normal group and the model group; F: KEGG enrichment pathway map of differential metabolic pathway between the treated group and the model group. Control group: without treatment; Model group: treated with lipopolysaccharide; Sample group: treated with lipopolysaccharide and non-volatile ingredients of patchouli of 10 mg/L. Student’s t-test was used for statistical significance (P < 0.05); Data are presented as mean ± standard deviation (n = 6). Significant differences compared with control group were designated as aP < 0.001 and with model group as bP < 0.001.
Figure 4 Metabolomics pathway analysis A: clustering heat map of differential metabolites in the normal group and the model group; B: clustering heat map of differential metabolites in the treated group and the model group; C: arginine levels in cell samples; D: sorbitol levels in cell samples; E: KEGG enrichment pathway map of differential metabolic pathway between the normal group and the model group; F: KEGG enrichment pathway map of differential metabolic pathway between the treated group and the model group. Control group: without treatment; Model group: treated with lipopolysaccharide; Sample group: treated with lipopolysaccharide and non-volatile ingredients of patchouli of 10 mg/L. Student’s t-test was used for statistical significance (P < 0.05); Data are presented as mean ± standard deviation (n = 6). Significant differences compared with control group were designated as aP < 0.001 and with model group as bP < 0.001.
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