Journal of Traditional Chinese Medicine ›› 2022, Vol. 42 ›› Issue (6): 900-907.DOI: 10.19852/j.cnki.jtcm.20220815.001
• Research Articles • Previous Articles Next Articles
HENG Xianpei(), LI Liang, YANG Liuqin, WANG Zhita
Received:
2021-07-07
Accepted:
2021-10-17
Online:
2022-12-15
Published:
2022-08-15
Contact:
HENG Xianpei,LI Liang,YANG Liuqin
About author:
Prof. HENG Xianpei, Department of Endocrinology, People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou 350004, China. hengxianpei@hotmail.com, Telephone: +86-13067366157Supported by:
HENG Xianpei, LI Liang, YANG Liuqin, WANG Zhita. Efficacy of Dangua Fang (丹瓜方) on endothelial cells damaged by oxidative stress[J]. Journal of Traditional Chinese Medicine, 2022, 42(6): 900-907.
Group | n | OD value | ReR (%) | Group | n | OD value | ReR (%) | Group | n | OD value | ReR (%) |
---|---|---|---|---|---|---|---|---|---|---|---|
Control | 3 | 0.5772±0.0321 | - | DG3 | 3 | 0.4113±0.0536c | 32.268 | DD1 | 3 | 0.3224±0.0113 | –7.076 |
Model | 3 | 0.3429±0.0313a | - | DZ1 | 3 | 0.4356±0.0213c | 42.393 | DD2 | 3 | 0.3542±0.0024 | 5.934 |
DG1 | 3 | 0.3778±0.0144 | 19.392 | DZ2 | 3 | 0.4242±0.0334c | 34.104 | DD3 | 3 | 0.3755±0.0157 | 19.323 |
DG2 | 3 | 0.3851±0.0329b | 20.478 | DZ3 | 3 | 0.3266±0.0278 | –1.977 |
Table 1 Comparison of OD values of different concentrations of Dangua Fang-containing serum intervention of endothelial cell proliferation activity injure induced by oxidative stress ($\bar{x} \pm s$)
Group | n | OD value | ReR (%) | Group | n | OD value | ReR (%) | Group | n | OD value | ReR (%) |
---|---|---|---|---|---|---|---|---|---|---|---|
Control | 3 | 0.5772±0.0321 | - | DG3 | 3 | 0.4113±0.0536c | 32.268 | DD1 | 3 | 0.3224±0.0113 | –7.076 |
Model | 3 | 0.3429±0.0313a | - | DZ1 | 3 | 0.4356±0.0213c | 42.393 | DD2 | 3 | 0.3542±0.0024 | 5.934 |
DG1 | 3 | 0.3778±0.0144 | 19.392 | DZ2 | 3 | 0.4242±0.0334c | 34.104 | DD3 | 3 | 0.3755±0.0157 | 19.323 |
DG2 | 3 | 0.3851±0.0329b | 20.478 | DZ3 | 3 | 0.3266±0.0278 | –1.977 |
Figure 1 oxidative stress-induced injured endothelial cells under different interventions (×200) Figures were photographed under an inverted microscope. A: conventional culture, no intervention; B: without drug intervention after modelling (1000μmol/L final concentration of H2O2-caused oxidative stress-damage); C: after modelling, treatment with Dangua Fang drug-containing serum; D: medium containing final concentrations of vitamin C of 20 μg/mL and coenzyme Q of 80 μg/mL. cells were cultured for 48 h.
Figure 2 Comparison of cell viability, apoptosis and cell cycle of oxidative stress-induced injured cell (n = 6,$\bar{x} \pm s$) A: comparison of cell viability; B: comparison of apoptosis; C: comparison of G2 + S. Control: conventional culture, no intervention; Model: without drug intervention after modelling (1000μmol/L final concentration of H2O2-caused oxidative stress-damage); Dangua: after modelling, treatment with Dangua Fang drug-containing serum; CoQ + Vit C: medium containing final concentrations of vitamin C of 20 μg/mL and coenzyme Q of 80 μg/mL. Cells were cultured for 48 h. compared with Control group: aP < 0.05; compared with Model group: bP < 0.01, cP < 0.05. G2 + S: Late stage of DNA synthesis + DNA synthesis stage.
Group | n | SDH | COX |
---|---|---|---|
Control | 3 | 9.73±1.13 | 1.11±0.07 |
Model | 3 | 11.91±0.77a | 0.78±0.04a |
Dangua | 3 | 12.63±0.30 | 0.90±0.07c |
CoQ+VitC | 3 | 6.92±0.18b | 0.82±0.04 |
Table 2 Influence of Dangua Fang on mitochondrial respiratory chain enzymes (μg/μL, $\bar{x} \pm s$)
Group | n | SDH | COX |
---|---|---|---|
Control | 3 | 9.73±1.13 | 1.11±0.07 |
Model | 3 | 11.91±0.77a | 0.78±0.04a |
Dangua | 3 | 12.63±0.30 | 0.90±0.07c |
CoQ+VitC | 3 | 6.92±0.18b | 0.82±0.04 |
Group | n | R3 Red/green (×100) | R2 Red/green (×100) | R1 Red/green (×100) | Green (R2 + R3) |
---|---|---|---|---|---|
Contol | 4 | 13.502±0.579 | 58.104±0.843 | 45.366±3.911 | 695.294±11.408 |
Model | 4 | 3.658±0.012a | 47.802±0.502a | 6.513±0.130a | 887.108±3.724a |
Dangua | 4 | 6.836±0.014b | 60.835±0.199b | 51.015±4.365b | 638.714±25.206b |
CoQ+VitC | 4 | 10.129±0.009b | 73.697±1.041b | 61.643±0.636b | 686.668±14.191b |
Table 3 Comparison of Mitochondrial Membrane Potential Parameters in all groups ($\bar{x} \pm s$)
Group | n | R3 Red/green (×100) | R2 Red/green (×100) | R1 Red/green (×100) | Green (R2 + R3) |
---|---|---|---|---|---|
Contol | 4 | 13.502±0.579 | 58.104±0.843 | 45.366±3.911 | 695.294±11.408 |
Model | 4 | 3.658±0.012a | 47.802±0.502a | 6.513±0.130a | 887.108±3.724a |
Dangua | 4 | 6.836±0.014b | 60.835±0.199b | 51.015±4.365b | 638.714±25.206b |
CoQ+VitC | 4 | 10.129±0.009b | 73.697±1.041b | 61.643±0.636b | 686.668±14.191b |
Figure 3 Comparison of Na+-k+-ATPase and PGC-1αin all groups (n = 3, $\bar{x} \pm s$) A: comparison of Na+-k+-ATPase: Control group: conventional culture, no intervention; Model group: without drug intervention after modelling (1000μmol/L final concentration of H2O2-caused oxidative stress-damage); Dangua group: after modelling, treatment with Dangua Fang drug-containing serum; Diazoxide group: the culture solution contained a final diazoxide concentration of 0.1 μmol/mL; Combined: diazoxide + Dangua Fang. B: Comparsion of PGC-1α. The treatments of the control group, model group and Dangua group were the same as in Figure 3A. CoQ + Vit C group: medium containing final concentrations of vitamin C of 20 μg/mL and coenzyme Q of 80 μg/mL. Cells were cultured for 48 h. Compared with Control group, aP < 0.01, cP < 0.05; compared with Model group, bP < 0.01. PGC-1α: peroxisome proliferators-activated receptor-γcoactivator-1.
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