Journal of Traditional Chinese Medicine ›› 2024, Vol. 44 ›› Issue (5): 954-962.DOI: 10.19852/j.cnki.jtcm.20240806.003
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Qingfei Zhisou oral liquid (清肺止嗽口服液) alleviates fever-induced inflammation by regulating arachidonic acid and lysophospholipids metabolism and inhibiting hypothalamus transient receptor potential ion channels expression
GAO Jiaming1, ZHANG Yehao1, Chen Yuanyuan1, JIN Long1, ZHAO Jianfeng2, GUO Hao1(
), FU Jianhua1()
- 1 Institute of Basic Medical Sciences, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing 100091, China
2 Pharmaceutical R&D Department, Taiyuan Yidavike Medical Technology Development Co., Ltd., Taiyuan 030006, China
-
Received:2023-05-06Accepted:2023-09-05Online:2024-10-15Published:2024-08-06 -
Contact:Prof. GUO Hao, Institute of Basic Medical Sciences, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing 100091, China. g0502g@163.com;Prof. FU Jianhua, Institute of Basic Medical Sciences, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing 100091, China. jianhuaffcn@263.net Telephone: +86-10-62835636; +86-10-62835016 -
Supported by:Beijing Traditional Chinese Medicine Foundation for Science and Technology(JJ-2020-78);Research on the Protection of Intellectual Property Rights of Traditional Chinese Medicine; Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(CI2021A00912);Basic and Translational Research on the Application of Traditional Chinese Medicine; the Research Foundation of Major New Drug Creation, from the Ministry of Science and Technology of China(2018ZX09721003-009-022);Research on Key Technologies and Industrialization of Traditional Chinese Medicine Preparations for Children;National Natural Science Foundation of China(82074060);Study on the Effect of Tanyu Tongzhi Fang in Maintaining Vascular Homeostasis in the Treatment of Atherosclerosis
Cite this article
GAO Jiaming, ZHANG Yehao, Chen Yuanyuan, JIN Long, ZHAO Jianfeng, GUO Hao, FU Jianhua. Qingfei Zhisou oral liquid (清肺止嗽口服液) alleviates fever-induced inflammation by regulating arachidonic acid and lysophospholipids metabolism and inhibiting hypothalamus transient receptor potential ion channels expression[J]. Journal of Traditional Chinese Medicine, 2024, 44(5): 954-962.
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Figure 1 Differences in inflammatory cytokines and hypothalamic-releasing factors A: IL-6 level; B: TNF-α level; C: PGE-2 level; D: cAMP level; E: AVP; F: rectal temperature of the rats in each group. Control group: sham operated; Model group: Yeast-induced fever model without treatment; Positive group: Yeast-induced fever model with Aspirin (100 mg/kg); Lose-dose QFZS group: Yeast-induced fever model with low-dose QFZS (2.82 g/kg); High-dose QFZS group: Yeast-induced fever model with high-dose QFZS (5.64 g/kg). IL-6: interleukin-6; TNF-α: tumor necrosis factor-α; AVP: arginine vasopressin; cAMP: cyclic adenosine monophosphate; PGE-2: prostaglandin E-2; QFZS: Qingfei Zhisou oral liquid. Data represent the mean ± standard deviation using one-way analysis of variance (n = 4). Compared with the control group, aP < 0.01, dP < 0.05; compared with the model group, bP < 0.05, cP < 0.01.
Figure 1 Differences in inflammatory cytokines and hypothalamic-releasing factors A: IL-6 level; B: TNF-α level; C: PGE-2 level; D: cAMP level; E: AVP; F: rectal temperature of the rats in each group. Control group: sham operated; Model group: Yeast-induced fever model without treatment; Positive group: Yeast-induced fever model with Aspirin (100 mg/kg); Lose-dose QFZS group: Yeast-induced fever model with low-dose QFZS (2.82 g/kg); High-dose QFZS group: Yeast-induced fever model with high-dose QFZS (5.64 g/kg). IL-6: interleukin-6; TNF-α: tumor necrosis factor-α; AVP: arginine vasopressin; cAMP: cyclic adenosine monophosphate; PGE-2: prostaglandin E-2; QFZS: Qingfei Zhisou oral liquid. Data represent the mean ± standard deviation using one-way analysis of variance (n = 4). Compared with the control group, aP < 0.01, dP < 0.05; compared with the model group, bP < 0.05, cP < 0.01.
Figure 2 Effects of QFZS on TRPM2 expression A: the immunohistochemistry staining of TRPM2, A1-A4: images of control (A1), model (A2), Lose-dose QFZS (A3), High-dose QFZS(A4) (× 1); A5-A8: images of control (A5), model (A6), Lose-dose QFZS (A7), High-dose QFZS(A8) (× 200, bar = 100 μm); A9-A12: images of control (A9), model (A10), Lose-dose QFZS (A11), High-dose QFZS(A12) (× 400, bar = 50 μm). B: Western blotting representative images of TRPM2 respective quantification in the hypothalamus; C: protein expression levels of TRPM2. Control group: sham operated; Model group: Yeast-induced fever model without treatment; Positive group: Yeast-induced fever model with Aspirin (100 mg/kg); Lose-dose QFZS group: Yeast-induced fever model with low-dose QFZS (2.82 g/kg); High-dose QFZS group: Yeast-induced fever model with high-dose QFZS (5.64 g/kg). TRPM2: transient receptor potential melastatin 2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; QFZS: Qingfei Zhisou oral liquid. Data represent the mean ± standard deviation using one-way analysis of variance (n = 5). Compared with the sham group, aP < 0.05; compared with the model group, bP < 0.05.
Figure 2 Effects of QFZS on TRPM2 expression A: the immunohistochemistry staining of TRPM2, A1-A4: images of control (A1), model (A2), Lose-dose QFZS (A3), High-dose QFZS(A4) (× 1); A5-A8: images of control (A5), model (A6), Lose-dose QFZS (A7), High-dose QFZS(A8) (× 200, bar = 100 μm); A9-A12: images of control (A9), model (A10), Lose-dose QFZS (A11), High-dose QFZS(A12) (× 400, bar = 50 μm). B: Western blotting representative images of TRPM2 respective quantification in the hypothalamus; C: protein expression levels of TRPM2. Control group: sham operated; Model group: Yeast-induced fever model without treatment; Positive group: Yeast-induced fever model with Aspirin (100 mg/kg); Lose-dose QFZS group: Yeast-induced fever model with low-dose QFZS (2.82 g/kg); High-dose QFZS group: Yeast-induced fever model with high-dose QFZS (5.64 g/kg). TRPM2: transient receptor potential melastatin 2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; QFZS: Qingfei Zhisou oral liquid. Data represent the mean ± standard deviation using one-way analysis of variance (n = 5). Compared with the sham group, aP < 0.05; compared with the model group, bP < 0.05.
Figure 3 Screening, pathway analysis, and enrichment of differential metabolites A: summary analysis of differential metabolites volcano plot, B; heat map of metabolisms, C: pathway enrichment of differential metabolites.
Figure 3 Screening, pathway analysis, and enrichment of differential metabolites A: summary analysis of differential metabolites volcano plot, B; heat map of metabolisms, C: pathway enrichment of differential metabolites.
Figure 4 Relative levels of common metabolites A: arachidonic acid; B: galactonic acid; C: N-desmethylcitalopram; D: Western blotting representative images of COX-2; E: protein expression levels of COX-2. Control group: sham operated; model group: Yeast-induced fever model without treatment; positive group: Yeast-induced fever model with Aspirin (100 mg/kg); Lose-dose QFZS group: Yeast-induced fever model with low-dose QFZS (2.82 g/kg); High-dose QFZS group: Yeast-induced fever model with high-dose QFZS (5.64 g/kg). COX-2: cyclooxygenase-2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; QFZS: Qingfei Zhisou oral liquid. Data represent the mean ± standard deviation using one-way analysis of variance (n = 5). Compared with the control, aP < 0.01, dP < 0.001; compared with the model, bP < 0.001, cP < 0.01.
Figure 4 Relative levels of common metabolites A: arachidonic acid; B: galactonic acid; C: N-desmethylcitalopram; D: Western blotting representative images of COX-2; E: protein expression levels of COX-2. Control group: sham operated; model group: Yeast-induced fever model without treatment; positive group: Yeast-induced fever model with Aspirin (100 mg/kg); Lose-dose QFZS group: Yeast-induced fever model with low-dose QFZS (2.82 g/kg); High-dose QFZS group: Yeast-induced fever model with high-dose QFZS (5.64 g/kg). COX-2: cyclooxygenase-2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; QFZS: Qingfei Zhisou oral liquid. Data represent the mean ± standard deviation using one-way analysis of variance (n = 5). Compared with the control, aP < 0.01, dP < 0.001; compared with the model, bP < 0.001, cP < 0.01.
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