Journal of Traditional Chinese Medicine ›› 2024, Vol. 44 ›› Issue (4): 745-752.DOI: 10.19852/j.cnki.jtcm.2024.04.002
• Original articles • Previous Articles Next Articles
SHEN Jie, YIN Yaoli, LI Hongxiao, LU Ge, ZHU Yaoyao, QIN Yantong, JIN Xun, CHENG Jie(), SHEN Meihong()
Received:
2023-03-02
Accepted:
2023-07-20
Online:
2024-08-15
Published:
2024-07-15
Contact:
Prof. CHENG Jie, College of Acupuncture and Tuina, Nanjing University of Chinese Medicine, Nanjing 210023, China. Supported by:
SHEN Jie, YIN Yaoli, LI Hongxiao, LU Ge, ZHU Yaoyao, QIN Yantong, JIN Xun, CHENG Jie, SHEN Meihong. Effect of moxibustion on expression profile of miRNAs in Tripterygium glycoside-induced decreased ovarian reserve[J]. Journal of Traditional Chinese Medicine, 2024, 44(4): 745-752.
Figure 1 Moxibustion has a protective effect on ovarian damage in TGS-induced DOR rats A-C: hematoxylin and eosin staining was used to detect the morphological changes in ovarian tissue. A1-C1: ×40. A2-C2: Enlarged part (×100). A-C: control, model, and moxibustion. Control group: rats treated with intragastric physiological saline (10 mL·kg-1·d-1) for 14 d; Model group: rats treated with TGS suspension (50 mg·kg-1·d-1) for 14 d; Moxibustion group: rats treated with TGS suspension (50 mg·kg-1·d-1) and moxibustion for 14 d. PF: primary follicle; SF: secondary follicle; MF: mature follicle; AF: atretic follicle; circles represent corpus luteum. D: number of follicles in each ovarian stage. The difference between the two groups was measured by Student’s t-test. Data are shown as the mean?±?standard deviation (n = 6). aP < 0.01, compared with the control group; bP < 0.05 and cP < 0.05, compared with the model group. DOR: decreased ovarian reserve; TGS: Tripterygium glycoside.
Figure 2 Effects of moxibustion on DOR on a transcriptome-wide scale A: volcano map of miRNA of the model versus the control groups; B: volcano map of miRNA of the moxibustion versus the model groups; C: venn diagram of the model versus the control (up) and the moxibustion versus the model (down) groups; D: venn diagram of the model versus the control (down) and the moxibustion versus the model (up) groups. Control group: rats treated with intragastric physiological saline (10 mL·kg-1·d-1) for 14 d; Model group: rats treated with TGS suspension (50 mg·kg-1·d-1) for 14 d; Moxibustion group: rats treated with TGS suspension (50 mg·kg-1·d-1) and moxibustion for 14 d. Mod: model; Con: control; Mox: moxibustion; DOR: decreased ovarian reserve; TGS: Tripterygium glycoside. The difference between the two groups was measured by Student’s t-test. Data are shown as the mean?±?standard deviation (n = 3).
Figure 3 Enrichment analysis of differential miRNA A: GO terms analysis of differential miRNA; B: pathway analysis of differential miRNA. GO: Gene Ontology; PI3K-Akt: phosphatidylinositol-3-kinase-protein kinase B; MAPK: mitogen-activated protein kinase; Nrf2/HO-1: nuclear factor erythropoietin-2-related factor 2/heme oxygenase 1; AMPK: adenosine 5‘-monophosphate (AMP)-activated protein kinase; GPI: glycosylphosphatidylinositol, mTOR: mammalian target of rapamycin; FDR: false discovery rate.
Figure 4 Construction of the miRNA-target gene-pathway and validation of miRNAs A: the network of miRNAs-target genes-pathway. The square represents the pathway; the diamond represents the mRNA; the octagon represents the miRNA. B: the differentially expressed miRNA was verified by RT-PCR. RT-PCR: reverse transcription polymerase chain reaction; Nrf2/ARE: nuclear factor erythropoietin-2-related factor 2/AU-rich element; PI3K/Akt: phosphatidylinositol-3-kinase/protein kinase B; Cdx2: caudal type homeobox 2; E2f1: E2 promoter binding factor 1; Atp5c1: adenosine triphosphate synthase subunit gamma; Sall1: spalt like transcription factor 1; Klf5: krüppel-like factor 5; Edn1: endothelin 1; Zfp382: zinc finger protein 382; Hmg20a: high mobility Group 20a; Bach2: BTB domain and CNC homolog 2; Bcl6b: B-cell Lymphoma 6b; Rnf2: ring finger protein 2; Cbx2: chromobox 2; Esr1: estrogen receptor 1; Shox2: short Stature homobox 2; Cav1: caveolin-1; Pias1: protein inhibitor of activated signal transducer and activators of transcription 1; Dcaf1: DDB1 and CUL4 associated factor 1. The difference between the two groups was measured by Student’s t-test. Data are shown as the mean?±?standard deviation (n = 5). aP < 0.05, compared with the control group. bP < 0.01 and cP < 0.05, compared with the model group.
1. | Greene AD, Patounakis G, Segars JH. Genetic associations with diminished ovarian reserve: a systematic review of the literature. J Assist Reprod Genet 2014; 31: 935-46. |
2. |
Zhao M, Feng F, Chu C, Yue W, Li L. A novel EIF4ENIF1 mutation associated with a diminished ovarian reserve and premature ovarian insufficiency identified by whole-exome sequencing. J Ovarian Res 2019; 12: 119.
DOI PMID |
3. | Tan Z, Gong X, Wang CC, et al. Diminished Ovarian Reserve in Endometriosis: Insights from In Vitro, In Vivo, and Human Studies-A Systematic Review. Int J Mol Sci 2023; 24(21): 15967. |
4. | Jin X, Cheng J, Shen J, et al. Moxibustion improves ovarian function based on the regulation of the androgen balance. Erratum in: Exp Ther Med 2022; 23:190. |
5. | Shen J, Ai B, Shen M. Effectiveness of mild moxibustion for sub-health conditions in pre- and post-menopausal women: a randomized controlled clinical trial. Med Sci Monit 2018; 24: 2907-11. |
6. | Zhao QY, Sun Y, Zhou J, et al. Effectiveness of herb-partitioned moxibustion combined with electroacupuncture on polycystic ovary syndrome in patients with symptom pattern of kidney deficiency and phlegm-dampne. J Tradit Chin Med 2021; 41: 985-93. |
7. |
Yang X, Wang W, Zhang Y, Wang J, Huang F. Moxibustion improves ovary function by suppressing apoptosis events and upregulating antioxidant defenses in natural aging ovary. Life Sci 2019; 229: 166-72.
DOI PMID |
8. |
Thabet E, Yusuf A, Abdelmonsif DA, Nabil I, Mourad G, Mehanna RA. Extracellular vesicles miRNA-21: a potential therapeutic tool in premature ovarian dysfunction. Mol Hum Reprod 2020; 26: 906-19.
DOI PMID |
9. | Zhang J, Xu Y, Liu H, Pan Z. MicroRNAs in ovarian follicular atresia and granulosa cell apoptosis. Reprod Biol Endocrinol 2019; 17: 9. |
10. | Wei C, Xiang S, Yu Y, Song J, Zheng M, Lian F. miR-221-3p regulates apoptosis of ovarian granulosa cells via targeting FOXO1 in older women with diminished ovarian reserve (DOR). Mol Reprod Dev 2021; 88: 251-60. |
11. | Wang ZQ, Cui YH, Huang Y, et al. Herb-partitioned moxibustion regulated the miRNA expression profile in the thyroid tissues of rats with experimental autoimmune thyroiditi. J Tradit Chin Med 2021; 41: 789-98. |
12. | Sun Y, Chen S, Tan Y. Influence of nourishing Yin and tonifying Yang sequential therapy combined with Western Medicine on TGF-β1/Smads signaling pathway in anovulatory infertility rats with diminished ovarian reserve. Zhong Nan Da Xue Xue Bao Yi Xue Ban 2018; 43: 1068-74. |
13. |
Hirshfield AN, Midgley AR Jr. Morphometric analysis of follicular development in the rat. Biol Reprod 1978; 19: 597-605.
PMID |
14. | Tilly JL. Ovarian follicle counts--not as simple as 1, 2, 3. Reprod Biol Endocrinol 2003; 1: 11. |
15. | Liu W, Chen Q, Liu Z, et al. Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis. J Ethnopharmacol 2021; 277: 113789. |
16. | Wang Q, Lu G, Xie Z, Xie ZJ, Li HX, Shen MH. Effect of moxibustion on Nrf2/HO-1 signaling pathway in rats with diminished ovarian reserve. Zhong Guo Zhen Jiu 2021; 41: 53-8. |
17. | Xiao J, Song J, Sa Y, Yuan L, Guo J, Sun Z. The mechanisms of improving IVF outcomes of Liu-Wei-Di-Huang pill acting on DOR patients. Evid Based Complement Alternat Med 2020; 2020: 5183017. |
18. | Yang LJ, Wu J, Yang L, Zhou T, Li HR, Miao RQ. Effect on follicular development and pregnancy outcome treated with acupuncture and moxibustion therapy of Tiaochongren Gushenyuan in patients with luteal phase defect. Zhong Guo Zhen Jiu 2019; 39: 927-31. |
19. | Li Y, Xia G, Tan Y, Shuai J. Acupoint stimulation and Chinese herbal medicines for the treatment of premature ovarian insufficiency: a systematic review and Meta-analysis. Complement Ther Clin Pract 2020; 41: 101244. |
20. |
Ren Y, Yang X, Zhang Y, Wang Y, Li X. Effects and mechanisms of acupuncture and moxibustion on reproductive endocrine function in male rats with partial androgen deficiency. Acupunct Med 2016; 34: 136-43.
DOI PMID |
21. | Fan H, Ji F, Lin Y, et al. Electroacupuncture stimulation at CV4 prevents ovariectomy-induced osteoporosis in rats via Wnt-β-catenin signaling. Mol Med Rep 2016; 13: 2485-91. |
22. |
Fu H, Sun J, Tan Y, et al. Effects of acupuncture on the levels of serum estradiol and pituitary estrogen receptor beta in a rat model of induced super ovulation. Life Sci 2018; 197: 109-13.
DOI PMID |
23. |
Du Y, Liu R, Sun G, Meng P, Song J. Pre-moxibustion and moxibustion prevent Alzheimer's disease. Neural Regen Res 2013; 8: 2811-9.
DOI PMID |
24. | Li HX, Shi L, Liang SJ, et al. Moxibustion alleviates decreased ovarian reserve in rats by restoring the PI3K/AKT signaling pathway. J Integr Med 2022; 20: 163-72. |
25. | Yin Y, Li H, Qin Y, et al. Moxibustion mitigates mitochondrial dysfunction and NLRP 3 inflammatory activation in cyclophosphamide-induced premature ovarian insufficiency rats. Life Sci 2023; 314: 121283. |
26. | Zhang YR, Huang W, Wang LH, Yu BL, Zhang YJ, Zhou ZY. Summary of professor Zhou Zhongyu's academic experience in the treatment of decreased ovarian reserve function with Yin-Yang regulating moxibustion. Zhen Jiu Lin Chuang Za Zhi 2023; 39: 86-90. |
27. |
Poulakos L, Kent TH. Gastric emptying and small intestinal propulsion in fed and fasted rats. Gastroenterology 1973; 64: 962-7.
PMID |
28. | Lu G, Wang Q, Xie ZJ, et al. Moxibustion ameliorates ovarian reserve in rats by mediating Nrf2/HO-1/NLRP 3 anti-inflammatory pathway. Evid Based Complement Alternat Med 2021; 2021: 8817858. |
29. | Zheng Y, Feng X, Mi H, et al. Effects of transcutaneous electrical acupoint stimulation on ovarian reserve of patients with diminished ovarian reserve in in vitro fertilization and embryo transfer cycles. J Obstet Gynaecol Res 2015; 41: 1905-11. |
30. | Zhu S, Wang Y, Chang X, Chen H, Jin X. The Protective effect of pre-moxibustion on reproductive hormones profile of rats with tripterygium glycosides-induced ovarian damage. Complement Med Res 2020; 27: 401-9. |
31. |
Hewlett M, Mahalingaiah S. Update on primary ovarian insufficiency. Curr Opin Endocrinol Diabetes Obes 2015; 22: 483-9.
DOI PMID |
32. |
Abu-Halima M, Becker LS, Ayesh BM, et al. Characterization of micro-RNA in women with different ovarian reserve. Sci Rep 2021; 11: 13351.
DOI PMID |
33. | Zhao C, Li X, Yang Y, et al. An analysis of Treg/Th 17 cells imbalance associated microRNA networks regulated by moxibustion therapy on Zusanli (ST36) and Shenshu (BL23) in mice with collagen induced arthritis. Am J Transl Res 2019; 11: 4029-45. |
34. |
Bouazzi L, Sproll P, Eid W, Biason-Lauber A. The transcriptional regulator CBX2 and ovarian function: a whole genome and whole transcriptome approach. Sci Rep 2019; 9: 17033.
DOI PMID |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||
Sponsored by China Association of Chinese Medicine
& China Academy of Chinese Medical Sciences
16 Nanxiaojie, Dongzhimen Nei, Beijing, China. 100700 Email: jtcmen@126.com
Copyright 2020 Journal of Traditional Chinese Medicine. All rights reserved.