Journal of Traditional Chinese Medicine ›› 2024, Vol. 44 ›› Issue (4): 762-769.DOI: 10.19852/j.cnki.jtcm.20240515.002
• Original articles • Previous Articles Next Articles
WU Zhiwei1,2, ZHU Qingguang1,2, KONG Lingjun3, SONG Pengfei3, ZHOU Xin1,2, GUO Guangxin4, ZHANG Shuaipan3, HE Tianxiang3, CHENG Yanbin1,2, FANG Min1,2,3()
Received:
2023-02-23
Accepted:
2023-07-14
Online:
2024-08-15
Published:
2024-05-15
Contact:
FANG Min, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China; Institute of Tuina, Shanghai Institute of Traditional Chinese Medicine, Shanghai 200437, China; Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. Supported by:
WU Zhiwei, ZHU Qingguang, KONG Lingjun, SONG Pengfei, ZHOU Xin, GUO Guangxin, ZHANG Shuaipan, HE Tianxiang, CHENG Yanbin, FANG Min. Tuina alleviates neuropathic pain through regulate the activation of microglia and the secretion of inflammatory cytokine in spinal cord[J]. Journal of Traditional Chinese Medicine, 2024, 44(4): 762-769.
Figure 1 Analgesic effects of tuina on PWT and PWL in SD rats with CCI A: the PWT in response to von Frey filaments; B: the PWL to a noxious thermal beam. Tuina treatment was performed each day from days 4 to 13 (total of 10 times). Both PWT and PWL were tested and evaluated among sham group, CCI-induced NPP model group (CCI group), and CCI-induced NPP model with tuina treatment group (Tuina group) at days -2, -1, 3, 7, 10 and 14. PWT: paw withdrawal threshold; PWL: paw withdrawal latency; SD: Sprague-Dawley; CCI: chronic constrictive injury; NPP: neuropathic pain. Two-way repeated measures analysis of variance followed by Bonferroni’s multiple comparison tests was used for PWT and PWL test. Data are expressed as the mean ± standard error of mean (n = 8 per group). aP < 0.001, dP < 0.05 vs the sham group; bP < 0.05, eP < 0.01, cP < 0.001 vs the CCI group.
Figure 2 Effect of Tuina on the activation of microglia in the ipsilateral SDH of CCI rats A: representative Immunofluorescence staining images of Iba1 in the ipsilateral SDH, Scale bar, 100 μm; A1: Iba1 expression of Sham in low magnification mirror; A2: Iba1 expression of Sham in high magnification mirror; A3: Iba1 expression of CCI in low magnification mirror; A4: Iba1 expression of CCI in high magnification mirror; A5: Iba1 expression of Tuina in low magnification mirror; A6: Iba1 expression of Tuina in high magnification mirror; B: area fraction of Iba1 in the ipsilateral SDH; C: protein levels of Iba-1 standardized by GAPDH. Sham group: CCI except for the ligation of the sciatic nerve; CCI group: CCI-induced NPP model group; Tuina group: CCI-induced NPP model with Tuina treatment group. Iba-1: Ionised calcium binding adaptor molecule 1; SDH: spinal dorsal horn; CCI: chronic constrictive injury; GAPDH: glyceraldehyde-3?phosphate dehydrogenase. One-way analysis of variance was utilized for area fraction and protein levels of Iba1. Data are expressed as the mean ± standard error of mean (n = 8 per group). aP < 0.001 vs the sham group; bP < 0.01, cP < 0.001 vs the CCI group.
Figure 3 Effect of Tuina on the polarization of activated microglial A: protein levels of CD68 standardized by GAPDH; B: concentration of IL-1β standardized by total protein (IL-1β / total protein × 100%); C: concentration of TNF-α standardized by total protein (TNF-α / total protein × 100%). Sham group: CCI except for the ligation of the sciatic nerve; CCI group: CCI-induced NPP model group; Tuina group: CCI-induced NPP model with Tuina treatment group. GAPDH: glyceraldehyde-3?phosphate dehydrogenase; IL-1β: interleukin-1β; TNF-α: tumor necrosis factor-α; CCI: chronic constrictive injury. One-way analysis of variance was utilized for protein levels of CD68, concentration of IL-1β and TNF-α. Data are expressed as the mean ± standard error of mean (n = 8 per group). aP < 0.001 vs the sham group; bP < 0.001, cP < 0.01 vs the CCI group.
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