Journal of Traditional Chinese Medicine ›› 2023, Vol. 43 ›› Issue (6): 1103-1109.DOI: 10.19852/j.cnki.jtcm.2023.06.001
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DU Zhongheng1, CONG Wenjie1, TANG Kejing1, ZHENG Qiqi1, SONG Zhiwei1, CHEN Yong1, YANG Su3, ZHANG Chunwu2, YE Tianshen1
Received:
2022-06-22
Accepted:
2022-09-21
Online:
2023-10-25
Published:
2023-11-01
Contact:
ZHANG Chunwu, Department of Traditional Chinese Orthopedics and Traumatology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. zcw6681@126.com; YE Tianshen, Department of Acupuncture, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. yetianshen@wmu.edu.cn. Telephone: +86-13706660570
Supported by:
DU Zhongheng, CONG Wenjie, TANG Kejing, ZHENG Qiqi, SONG Zhiwei, CHEN Yong, YANG Su, ZHANG Chunwu, YE Tianshen. Electroacupuncture stimulating Zusanli (ST36), Sanyinjiao (SP6) in mice with collagen-induced arthritis leads to adenosine A2A receptor-mediated alteration of p38α mitogen-activated protein kinase signaling and inhibition of osteoclastogenesis[J]. Journal of Traditional Chinese Medicine, 2023, 43(6): 1103-1109.
Figure 1 Expression of p38α, NF-κB, NFATc1 in the ankle joints of mice in the different treatment groups A: expression of p38α in Blank group (× 100); B: expression of p38α in CIA-control group (× 100); C: expression of p38α in CIA-EA group (× 100); D: expression of p38α in CIA-EA-SCH58261 group (× 100); E: quantitation of p38α in all mice; F: expression of NF-κB in Blank group (× 100); G: expression of NF-κB in CIA-control group (× 100); H: expression of NF-κB in CIA-EA group (× 100); I: expression of NF-κB in CIA-EA-SCH58261 group (× 100); J: quantitation of NF-κB in all mice; K: expression of NFATc1 in Blank group (× 100); L: expression of NFATc1 in CIA-control group (× 100); M: expression of NFATc1 in CIA-EA group (× 100); N: expression of NFATc1 in CIA-EA-SCH58261 group (× 100); O: quantitation of NFATc1 in all mice. NF-κB: nuclear transcription factor-κB; NFATc1: nuclear factor of activated T cells C1; CIA: collagen induced arthritis; EA: electroacupuncture. Dyeing method of all pictures are the immunohistochemical SP method, PBS was used as negative control. Blank group: not have CIA and daily headgear fixation 30 min for 14 d (n =10); CIA-control group: become a CIA model and daily headgear fixation 30 min for 14 d (n = 10); CIA-EA group: become a CIA model, electroacupuncture stimulating Zusanli (ST36), Sanyinjiao (SP6) and daily headgear fixation 30 min at the same time for 14 d (n = 10); CIA-EA-SCH58261 group: received intraperitoneal injections of SCH58261 (adenosine A2A receptor antagonist, 5 mg/kg), and then headgear fixation, EA as in the CIA-EA group (n = 10). aP < 0.05, compared with the Blank group. bP < 0.05, compared with the CIA-control group. cP < 0.05, compared with the CIA-EA group. Data were compared using a one-way analysis of variance.
Figure 2 Western blotting of proteins in the p38α-MAPK signaling pathway in the different treatment groups 1: Blank group, not have CIA and daily headgear fixation 30 min for 14 d; 2: CIA-control group, become a CIA model and daily headgear fixation 30 min for 14 d; 3: CIA-EA group, become a CIA model, electroacupuncture stimulating Zusanli (ST36), Sanyinjiao (SP6) and daily headgear fixation 30 min at the same time for 14 d; 4: CIA-EA-SCH58261 group, received intraperitoneal injections of SCH58261 (Adenosine A2A receptor antagonist, 5 mg/kg), and then headgear fixation, EA as in the CIA-EA group. RANKL: receptor activator of nuclear transcription factor-κB ligand; RANK: receptor activator of NF-κB; TRAF6: tumor necrosis factor receptor associated factor 6; NF-κB: nuclear transcription factor-κB; NFATc1: nuclear factor of activated T cells C1; MAPK: mitogen-activated protein kinase; CIA: collagen induced arthritis; EA: electroacupuncture.
Figure 3 TRAP-staining of osteoclasts in representative mice from the different treatment groups A: expression of osteoclasts in Blank group (× 200); B: expression of osteoclasts in CIA-control group (× 200); C: expression of osteoclasts in CIA-EA group (× 200); D: expression of osteoclasts in CIA-EA-SCH58261 group (× 200). E: quantitation of osteoclasts in all mice. TRAP: tartrate-resistant acid phosphatase; CIA: collagen induced arthritis; EA: electroacupuncture. Dyeing method of all pictures are the TRAP staining method. Blank group: not have CIA and daily headgear fixation 30 min for 14 d (n = 10); CIA-control group: become a CIA model and daily headgear fixation 30 min for 14 d (n = 10); CIA-EA group: become a CIA model, electroacupuncture stimulating Zusanli (ST36), Sanyinjiao (SP6) and daily headgear fixation 30 min at the same time for 14 d (n = 10); CIA-EA-SCH58261 group: received intraperitoneal injections of SCH58261 (Adenosine A2A receptor antagonist, 5 mg/kg), and then headgear fixation, EA as in the CIA-EA group (n = 10). Data were compared using a one-way analysis of variance. aP < 0.05, compared with the Blank group; bP < 0.05, compared with the CIA-control group. cP < 0.05, compared with the CIA-EA group.
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