Journal of Traditional Chinese Medicine ›› 2024, Vol. 44 ›› Issue (5): 926-933.DOI: 10.19852/j.cnki.jtcm.2024.05.003
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Protective effect of Zhizi Huangqi Shanzha formula (栀子黄芪山楂方) on aflatoxin poisoning in mice and its effect on intestinal flora
SUN Chuanbo, XU Guangpei, JIANG Ping, HUANG Shipping, CHEN Cunwu, HE Yanfei(
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- College of Biotechnology and Pharmaceutical Engineering, West Anhui University, Lu’an 237012, China
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Received:2023-03-12Accepted:2023-07-14Online:2024-10-15Published:2024-09-11 -
Contact:HE Yanfei, College of Biotechnology and Pharmaceutical Engineering, West Anhui University, Lu’an 237012, China. 341281a00ek.cdb@sina.cn Telephone: +86-15956792864 -
Supported by:Provincial Natural Science Foundation of Anhui Province-Quantitative Constitutive Effect and Mechanism of Action of Dendrobium Polysaccharides against Tumours based on the Tumour Microenvironment(2208085QH273);Natural Science Research Project for Anhui Universities-mechanism of Anti-bacterial Diarrhea Effect of Probiotic-fermented Portulaca Oleracea in Targeting and Regulating Amino acid Metabolism of Intestinal Flora and Screening of Anti-tumor Active Fraction of Paris polyphylla and its Effect on the Tumour Immune Microenvironment(2023AH052634);Natural Science Research Project for Anhui Universities-mechanism of Anti-bacterial Diarrhea Effect of Probiotic-fermented Portulaca Oleracea in Targeting and Regulating Amino acid Metabolism of Intestinal Flora and Screening of Anti-tumor Active Fraction of Paris polyphylla and its Effect on the Tumour Immune Microenvironment(2022AH051668)
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SUN Chuanbo, XU Guangpei, JIANG Ping, HUANG Shipping, CHEN Cunwu, HE Yanfei. Protective effect of Zhizi Huangqi Shanzha formula (栀子黄芪山楂方) on aflatoxin poisoning in mice and its effect on intestinal flora[J]. Journal of Traditional Chinese Medicine, 2024, 44(5): 926-933.
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Figure 1 Effect of ZHSF on the blood cell composition of mice poisoned with AFB1 A: percentage of lymphocyte in the groups. B: percentage of eosinophils in the groups. C: percentage of monocyte in the groups. D: percentage of neutrophils in the groups. Control group: mice were orally administered (p.o.) with saline for 10 d. Model group: mice were orally administered (p.o.) with AFB1 of 0.4 mg/kg from the first day to the third day and orally administered (p.o.) with saline for 7 d before the sample harvesting. AFB1 + ZHSF (1.5 g/kg) group: mice were orally administered (p.o.) with AFB1 of 0.4 mg/kg from the first day to the third day and orally administered (p.o.) with ZHSF (1.5 g/kg) for 7 d before the sample harvesting. AFB1 + ZHSF (3.0 g/kg) group: mice were orally administered (p.o.) with AFB1 of 0.4 mg/kg from the first day to the third day and orally administered (p.o.) with ZHSF (3.0 g/kg) for 7 d before the sample harvesting. AFB1 + ZHSF (6.0 g/kg) group: mice were orally administered (p.o.) with AFB1 of 0.4 mg/kg from the first day to the third day and orally administered (p.o.) with ZHSF (6.0 g/kg) for 7 d before the sample harvesting. Blood routine analysis was used to analyze the blood cell composition in control group, model group, positive group and ZHSF groups. ZHSF: Zhizi Huangqi Shanzha formula; AFB1: aflatoxin B1; Lym: lymphocyte; Mon: monocyte; Eos: eosinophils; Neu: neutrophils. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 5). aP<0.05 compared to the model group.
Figure 1 Effect of ZHSF on the blood cell composition of mice poisoned with AFB1 A: percentage of lymphocyte in the groups. B: percentage of eosinophils in the groups. C: percentage of monocyte in the groups. D: percentage of neutrophils in the groups. Control group: mice were orally administered (p.o.) with saline for 10 d. Model group: mice were orally administered (p.o.) with AFB1 of 0.4 mg/kg from the first day to the third day and orally administered (p.o.) with saline for 7 d before the sample harvesting. AFB1 + ZHSF (1.5 g/kg) group: mice were orally administered (p.o.) with AFB1 of 0.4 mg/kg from the first day to the third day and orally administered (p.o.) with ZHSF (1.5 g/kg) for 7 d before the sample harvesting. AFB1 + ZHSF (3.0 g/kg) group: mice were orally administered (p.o.) with AFB1 of 0.4 mg/kg from the first day to the third day and orally administered (p.o.) with ZHSF (3.0 g/kg) for 7 d before the sample harvesting. AFB1 + ZHSF (6.0 g/kg) group: mice were orally administered (p.o.) with AFB1 of 0.4 mg/kg from the first day to the third day and orally administered (p.o.) with ZHSF (6.0 g/kg) for 7 d before the sample harvesting. Blood routine analysis was used to analyze the blood cell composition in control group, model group, positive group and ZHSF groups. ZHSF: Zhizi Huangqi Shanzha formula; AFB1: aflatoxin B1; Lym: lymphocyte; Mon: monocyte; Eos: eosinophils; Neu: neutrophils. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 5). aP<0.05 compared to the model group.
Figure 2 Effect of ZHSF on liver function and oxidative stress of AFB1 poisoning mice A: hematoxylin-eosin staining was used to analyze morphological and histological of liver in control group, model group, positive group and ZHSF groups. A1: Control group; A2: Model group; A3: Positive group; A4: ZHSF (1.5 g/kg) group; A5: ZHSF (3.0 g/kg) group; A6: ZHSF (6.0 g/kg) group. B: result of ALT; C: result of AST; D: result of SOD; E: result of MDA. Control group: Mice were orally administered (p.o.) with saline for 10 d. Model group: mice were orally administered (p.o.) with AFB1 of 0.4 mg/kg from the first day to the third day and orally administered (p.o.) with saline for 7 d before the sample harvesting. AFB1 + ZHSF (1.5 g/kg) group: mice were orally administered (p.o.) with AFB1 of 0.4 mg/kg from the first day to the third day and orally administered (p.o.) with ZHSF (1.5 g/kg) for 7 d before the sample harvesting. AFB1 + ZHSF (3.0 g/kg) group: mice were orally administered (p.o.) with AFB1 of 0.4 mg/kg from the first day to the third day and orally administered (p.o.) with ZHSF (3.0 g/kg) for 7 d before the sample harvesting. AFB1 + ZHSF (6.0 g/kg) group: mice were orally administered (p.o.) with AFB1 of 0.4 mg/kg from the first day to the third day and orally administered (p.o.) with ZHSF (6.0 g/kg) for 7 d before the sample harvesting. ZHSF: Zhizi Huangqi Shanzha formula; AFB1: aflatoxin B1; ALT: alanine aminotransferase; AST: aspartate aminotransferase; SOD: superoxide dismutase; MDA: malondialdehyde. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 5). aP<0.05 compared to the model group.
Figure 2 Effect of ZHSF on liver function and oxidative stress of AFB1 poisoning mice A: hematoxylin-eosin staining was used to analyze morphological and histological of liver in control group, model group, positive group and ZHSF groups. A1: Control group; A2: Model group; A3: Positive group; A4: ZHSF (1.5 g/kg) group; A5: ZHSF (3.0 g/kg) group; A6: ZHSF (6.0 g/kg) group. B: result of ALT; C: result of AST; D: result of SOD; E: result of MDA. Control group: Mice were orally administered (p.o.) with saline for 10 d. Model group: mice were orally administered (p.o.) with AFB1 of 0.4 mg/kg from the first day to the third day and orally administered (p.o.) with saline for 7 d before the sample harvesting. AFB1 + ZHSF (1.5 g/kg) group: mice were orally administered (p.o.) with AFB1 of 0.4 mg/kg from the first day to the third day and orally administered (p.o.) with ZHSF (1.5 g/kg) for 7 d before the sample harvesting. AFB1 + ZHSF (3.0 g/kg) group: mice were orally administered (p.o.) with AFB1 of 0.4 mg/kg from the first day to the third day and orally administered (p.o.) with ZHSF (3.0 g/kg) for 7 d before the sample harvesting. AFB1 + ZHSF (6.0 g/kg) group: mice were orally administered (p.o.) with AFB1 of 0.4 mg/kg from the first day to the third day and orally administered (p.o.) with ZHSF (6.0 g/kg) for 7 d before the sample harvesting. ZHSF: Zhizi Huangqi Shanzha formula; AFB1: aflatoxin B1; ALT: alanine aminotransferase; AST: aspartate aminotransferase; SOD: superoxide dismutase; MDA: malondialdehyde. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 5). aP<0.05 compared to the model group.
Table 1 Effect of ZHSF on the content of serum IgG and IgM mice poisoned with AFB1
| Item | Control group | Model group | Positive group | ZHSF (g/kg) | ||
|---|---|---|---|---|---|---|
| 1.5 | 3 | 6 | ||||
| IgG (μg/mL) | 1556±65a | 1259±118 | 1625±96a | 1508±31a | 1323±106a | 1325±144 |
| IgM (μg/mL) | 223±37a | 92±21 | 129±31 | 159±13a | 117±16a | 128±12a |
Table 1 Effect of ZHSF on the content of serum IgG and IgM mice poisoned with AFB1
| Item | Control group | Model group | Positive group | ZHSF (g/kg) | ||
|---|---|---|---|---|---|---|
| 1.5 | 3 | 6 | ||||
| IgG (μg/mL) | 1556±65a | 1259±118 | 1625±96a | 1508±31a | 1323±106a | 1325±144 |
| IgM (μg/mL) | 223±37a | 92±21 | 129±31 | 159±13a | 117±16a | 128±12a |
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