Anti-oxidative and immunological role of Cyclocarya paliurus polysaccharide on the liver injury of diabetic rats

doi:10.19852/j.cnki.jtcm.2024.06.005

Abstract

Abstract:

OBJECTIVE: To investigate the effects of Cyclocarya paliurus (C. paliurus) polysaccharide on the liver injury of diabetic rats.

METHODS: Rats were divided into 6 groups, including normal group, model group, control group, low-dose group of C. paliurus polysaccharide treatment, middle-dose group of C. paliurus polysaccharide treatment and high-dose group of C. paliurus polysaccharide treatment. Histological analysis of liver was analyzed using hematoxilin and eosin. Levels of plasma biological parameters and anti-oxidative enzymes were determined by spectrophotometry. Nuclear factor kappa-B-p65 (NF-κB p65), tumor necrosis factor-α (TNF-α), interleukins 6 (IL-6), IL-1β were measured by real-time polymerase chain reaction and enzyme-linked immunosorbent assay.

RESULTS: Compared with that of model group, the glucose level of plasma decreased 62.32% (P < 0.01), but glycogen and insulin level increased 1.51 times and 1.27 times in the high-dose group of C. paliurus polysaccharide treatment, respectively. Plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) level decreased 47.47% (P < 0.05), 43.65% (P < 0.05) and 50.51% (P < 0.05) in the high-dose group of C. paliurus polysaccharide treatment, respectively. Superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase level increased 1.16 times (P < 0.01), 71.28% (P < 0.05), 1.29 times (P < 0.01) and 87.46% (P < 0.05) in the high-dose group of C. paliurus polysaccharide treatment, respectively. Enzyme activities of NF-κB, TNF-α, IL-1β and IL-6 were decreased 39.29% (P < 0.05), 51.11% (P < 0.05), 37.42% (P < 0.05) and 36.50% (P < 0.05), respectively.

CONCLUSIONS: Administration of C. paliurus polysaccharide may play a protecting role for liver injury of diabetic rats through lowering glucose, ALT, AST, ALP level, increasing glycogen and insulin level, enhancing the anti-oxidative ability and down-regulating the inflammatory factors expression.

Key words: Cyclocarya paliurus polysaccharide, liver injury, diabetic rats, antioxidants, inflammatory factors

XIA Xichao, XUE Shipeng, SONG Guoying, LI Bin, WANG Huiping, QIU Ju, Wang Jihong, LIU Qingchun, MA Yuhong, OUYANG Jingfeng. Anti-oxidative and immunological role of Cyclocarya paliurus polysaccharide on the liver injury of diabetic rats[J]. Journal of Traditional Chinese Medicine, 2024, 44(6): 1146-1152.

Figures/Tables 5

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Group	n	SOD	CAT	GSH-Px	GR
Normal Diabetic control Metformin Low-dose	10 10 10 10	8.79±10.72 3.67±4.08^a 7.18±9.15^b 4.28±5.47^c	79.68±12.23 44.29±6.18^c 77.31±14.59^d 52.31±6.61^c	9.15±1.58 3.48±0.57^a 8.76±1.03^b 5.27±0.65^b	86.07±10.25 41.25±5.72^a 74.56±8.59^d 57.11±6.58^c
Middle-dose High-dose	10 10	5.67±6.53^cd 7.95±8.02^b	64.57±7.29^cd 75.86±8.73^d	6.38±0.65^cd 7.98±0.94^b	68.89±7.85^cd 77.33±9.40^d

Group	n	SOD	CAT	GSH-Px	GR
Normal Diabetic control Metformin Low-dose	10 10 10 10	8.79±10.72 3.67±4.08^a 7.18±9.15^b 4.28±5.47^c	79.68±12.23 44.29±6.18^c 77.31±14.59^d 52.31±6.61^c	9.15±1.58 3.48±0.57^a 8.76±1.03^b 5.27±0.65^b	86.07±10.25 41.25±5.72^a 74.56±8.59^d 57.11±6.58^c
Middle-dose High-dose	10 10	5.67±6.53^cd 7.95±8.02^b	64.57±7.29^cd 75.86±8.73^d	6.38±0.65^cd 7.98±0.94^b	68.89±7.85^cd 77.33±9.40^d

Group	n	NF-κB	TNF-α	IL-1β	IL-6
Normal Diabetic control Metformin Low-dose	10 10 10 10	0.53±0.05 3.94±0.43^a 0.69±0.07^b 2.52±0.29^ac	0.66±0.06 4.17±0.48^a 1.23±0.14^cd 3.76±0.40^a	0.47±0.05 5.93±0.63^a 1.61±0.20^ab 4.25±0.46^ac	1.07±0.09 14.79±1.33^a 2.41±0.29^cd 9.36±1.07^ac
Middle-dose High-dose	10 10	1.64±0.21^ab 0.89±0.09^b	2.65±0.30^ac 1.36±0.16^cd	3.01±0.34^ab 1.55±0.18^ab	6.42±0.68^ab 3.59±0.40^cd

Group	n	NF-κB	TNF-α	IL-1β	IL-6
Normal Diabetic control Metformin Low-dose	10 10 10 10	0.53±0.05 3.94±0.43^a 0.69±0.07^b 2.52±0.29^ac	0.66±0.06 4.17±0.48^a 1.23±0.14^cd 3.76±0.40^a	0.47±0.05 5.93±0.63^a 1.61±0.20^ab 4.25±0.46^ac	1.07±0.09 14.79±1.33^a 2.41±0.29^cd 9.36±1.07^ac
Middle-dose High-dose	10 10	1.64±0.21^ab 0.89±0.09^b	2.65±0.30^ac 1.36±0.16^cd	3.01±0.34^ab 1.55±0.18^ab	6.42±0.68^ab 3.59±0.40^cd

Group	n	NF-κB	TNF-α	IL-1β	IL-6
Normal Diabetic control Metformin Low-dose	10 10 10 10	189.2±27.5 361.5±31.2^a 203.9±23.4^b 324.3±24.6^c	24.6±2.2 56.0±3.4^a 28.2±3.2^b 49.5±5.7^a	18.7±2.3 34.6±4.1^c 21.6±2.6^b 27.1±3.6^bc	28.9±2.4 52.4±6.4^c 32.7±3.7^b 48.2±4.9^c
Middle-dose High-dose	10 10	267.2±32.7^bc 219.4±6.4^b	32.1±3.5^bc 27.4±3.2^d	23.8±2.4^bc 21.7±3.5^b	42.9±5.1^bc 33.3±3.6^b

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