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Journal of Traditional Chinese Medicine ›› 2020, Vol. 40 ›› Issue (5): 782-791.DOI: 10.19852/j.cnki.jtcm.2020.05.008

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Curcumin from Jianghuang (Rhizoma Curcumae Longae) protects against exposure to ultraviolet B by antioxidation and attenuating mitochondrion-dependent apoptosis

Hou Jiguang, Fang Fang, Kang Shunai, Wang Zhicheng, Yang Yanming   

  1. Department of Radiotherapy,Second Hospital of Jilin University;NHC Key laboratory of Radiobiology (Jilin University), School of Public Health, Jilin University;
  • Online:2020-10-15 Published:2020-10-15
  • Supported by:
    Supported by grants from Jilin Health Technology Innovation (No. 2019J069), Science and Technology Research Projects of the Education Department of Jilin Province (No. JJKH20180189KJ), the Science and Technology Development Plan of Jilin (No. 20180101305JC)

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Abstract: OBJECTIVE: To investigate the protective effect of curcumin extracted from Jianghuang(Rhizoma Curcumae Longae) against ultraviolet B(UVB) and the possible mechanism.METHODS: Effects of curcumin were detected in vivo and in vitro. Morphological changes of white guinea pig skin were assessed by hematoxylin and eosin staining. Ha CaT cell proliferation was measured by 3-[4,5-dimethylthylthiazol-2-yl]-2,5 diphenyltetrazolium broide(MTT) assays. The cell cycle distribution, apoptotic rate, level of reactive oxygen species(ROS), mitochondrial membrane potential, and intracellullar calcium ion concentration of Ha CaT cells were detected by flow cytometry. Antioxidant levels in skin tissues and Ha Cat cells were measured by biochemical methods.RESULTS: UVB inhibited in vitro cell proliferation by inducing G2/M arrest, increasing ROS, apoptosis,and necrosis, and decreasing B-cell lymphoma-2,and increasing Bax, cytochrome c, and caspase-3 levels.CONCLUSION: Curcumin blocks the effects of UVB by reducing ROS and apoptosis, and reversing UVB-induced changes in the expression of apoptotic proteins. The mitochondrial pathway is involved in curcumin-regulated apoptosis.

Key words: Ultraviolet rays, Curcumin, Antioxidants, Apoptosis, Mitochondria

Cite this article

Hou Jiguang, Fang Fang, Kang Shunai, Wang Zhicheng, Yang Yanming. Curcumin from Jianghuang (Rhizoma Curcumae Longae) protects against exposure to ultraviolet B by antioxidation and attenuating mitochondrion-dependent apoptosis[J]. Journal of Traditional Chinese Medicine, 2020, 40(5): 782-791.