Journal of Traditional Chinese Medicine ›› 2023, Vol. 43 ›› Issue (4): 725-733.DOI: 10.19852/j.cnki.jtcm.2023.04.007
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LIU Di1, ZHANG Yingqi1(), YU Tianyuan1(), LIU Zhifeng1, JIAO Yi1, WANG Hourong1, XU Yajing1, GUAN Qian1, CHEN Lulu2, HU Hui3
Received:
2022-07-22
Accepted:
2022-11-15
Online:
2023-08-15
Published:
2023-07-03
Contact:
ZHANG Yingqi, School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 102401, China. zhangyingqi0305@163.com. Telephone: +86-18210220868; +86-15810409565Supported by:
LIU Di, ZHANG Yingqi, YU Tianyuan, LIU Zhifeng, JIAO Yi, WANG Hourong, XU Yajing, GUAN Qian, CHEN Lulu, HU Hui. Protective mechanisms of Tuina therapy against lipopolysaccharide-induced fever in young rabbits based on untargeted metabolomics analysis[J]. Journal of Traditional Chinese Medicine, 2023, 43(4): 725-733.
Group | n | Anal temperature (℃) | |||
---|---|---|---|---|---|
0 h | 1 h | 2 h | 3 h | ||
Saline | 8 | 39.10±0.23 | 39.06±0.16 | 39.05±0.14 | 39.09±0.11 |
Model | 8 | 39.09±0.18 | 40.09±0.23 | 40.31±0.33 | 40.66±0.49 |
Tuina | 8 | 39.19±0.06 | 40.10±0.05 | 39.40±0.32a | 39.64±0.46a |
Table 1 Comparison of the anal temperature of young rabbits in 3 groups ($\bar{x}±s$)
Group | n | Anal temperature (℃) | |||
---|---|---|---|---|---|
0 h | 1 h | 2 h | 3 h | ||
Saline | 8 | 39.10±0.23 | 39.06±0.16 | 39.05±0.14 | 39.09±0.11 |
Model | 8 | 39.09±0.18 | 40.09±0.23 | 40.31±0.33 | 40.66±0.49 |
Tuina | 8 | 39.19±0.06 | 40.10±0.05 | 39.40±0.32a | 39.64±0.46a |
Group | n | TRI1 | 120 min TRI2 |
---|---|---|---|
Saline Model Tuina | 8 | 0.16±0.14 1.61±0.45a 1.49±0.22b | 0.82±0.64 9.14±2.29a 4.34±1.28ab |
8 | |||
8 |
Table 2 Comparison of TRI1 and TRI2 in 3 groups($\bar{x}±s$)
Group | n | TRI1 | 120 min TRI2 |
---|---|---|---|
Saline Model Tuina | 8 | 0.16±0.14 1.61±0.45a 1.49±0.22b | 0.82±0.64 9.14±2.29a 4.34±1.28ab |
8 | |||
8 |
Group | n | Blood | Hypothalamus |
---|---|---|---|
Saline | 8 | 587±22 667±8a 634±24ab | 635±42 730±19b 686±9ab |
Model | 8 | ||
Tuina | 8 |
Table 3 Comparison of PGE2 expression in the blood and hypothalamus in 3 groups (pg/mL)
Group | n | Blood | Hypothalamus |
---|---|---|---|
Saline | 8 | 587±22 667±8a 634±24ab | 635±42 730±19b 686±9ab |
Model | 8 | ||
Tuina | 8 |
Figure 2 Analysis of the potential biomarkers A: heat map of the hierarchical cluster analysis of the Tuina group vs the model group; B: heat map of the hierarchical cluster analysis of the saline group vs the model group.
Metabolite name | mz | VIP value | P value | Fold Change | Trend |
---|---|---|---|---|---|
Myo-inositol | 203.05 | 1.66 | 0.04 | 1.40 | ↑ |
Nicotinamide N-oxide | 139.05 | 2.44 | 0.03 | 2.42 | ↑ |
2, 4-Dimethyl-5-vinylthiazole | 140.05 | 2.40 | 0.03 | 3.27 | ↑ |
Gamma-aminobutyric acid | 104.07 | 1.58 | 0.05 | 1.23 | ↑ |
PC [20:4(8Z, 11Z, 14Z, 17Z)/P-18:0] | 794.61 | 1.90 | 0.03 | 0.80 | ↓ |
PC [18:1(11Z)/14:0] | 732.55 | 1.79 | 0.04 | 1.79 | ↑ |
PC (20:0/14:0) | 762.59 | 1.57 | 0.04 | 1.39 | ↑ |
SM (d18:1/20:0) | 759.64 | 1.63 | 0.04 | 1.19 | ↑ |
Armillaramide | 556.53 | 1.83 | 0.04 | 1.33 | ↑ |
PI [20:1(11Z)/18:2(9Z, 12Z)] | 889.57 | 2.08 | 0.01 | 1.34 | ↑ |
PI [22:4(10Z, 13Z, 16Z, 19Z)/16:0] | 887.56 | 1.84 | 0.02 | 1.24 | ↑ |
DG [20:3(8Z, 11Z, 14Z)/18:3(6Z, 9Z, 12Z)/0:0] | 641.51 | 1.90 | 0.02 | 0.71 | ↓ |
5-Hydroxyindoleacetic acid | 192.06 | 1.60 | 0.04 | 0.73 | ↓ |
LysoPC(22:0) | 580.43 | 1.71 | 0.04 | 0.74 | ↓ |
(?±)-2, 2'-Iminobispropanoic acid | 162.08 | 1.93 | 0.02 | 0.72 | ↓ |
Porphobilinogen | 227.10 | 1.72 | 0.04 | 0.73 | ↓ |
Table 4 Results of differential metabolite screening of the Tuina group versus the model group
Metabolite name | mz | VIP value | P value | Fold Change | Trend |
---|---|---|---|---|---|
Myo-inositol | 203.05 | 1.66 | 0.04 | 1.40 | ↑ |
Nicotinamide N-oxide | 139.05 | 2.44 | 0.03 | 2.42 | ↑ |
2, 4-Dimethyl-5-vinylthiazole | 140.05 | 2.40 | 0.03 | 3.27 | ↑ |
Gamma-aminobutyric acid | 104.07 | 1.58 | 0.05 | 1.23 | ↑ |
PC [20:4(8Z, 11Z, 14Z, 17Z)/P-18:0] | 794.61 | 1.90 | 0.03 | 0.80 | ↓ |
PC [18:1(11Z)/14:0] | 732.55 | 1.79 | 0.04 | 1.79 | ↑ |
PC (20:0/14:0) | 762.59 | 1.57 | 0.04 | 1.39 | ↑ |
SM (d18:1/20:0) | 759.64 | 1.63 | 0.04 | 1.19 | ↑ |
Armillaramide | 556.53 | 1.83 | 0.04 | 1.33 | ↑ |
PI [20:1(11Z)/18:2(9Z, 12Z)] | 889.57 | 2.08 | 0.01 | 1.34 | ↑ |
PI [22:4(10Z, 13Z, 16Z, 19Z)/16:0] | 887.56 | 1.84 | 0.02 | 1.24 | ↑ |
DG [20:3(8Z, 11Z, 14Z)/18:3(6Z, 9Z, 12Z)/0:0] | 641.51 | 1.90 | 0.02 | 0.71 | ↓ |
5-Hydroxyindoleacetic acid | 192.06 | 1.60 | 0.04 | 0.73 | ↓ |
LysoPC(22:0) | 580.43 | 1.71 | 0.04 | 0.74 | ↓ |
(?±)-2, 2'-Iminobispropanoic acid | 162.08 | 1.93 | 0.02 | 0.72 | ↓ |
Porphobilinogen | 227.10 | 1.72 | 0.04 | 0.73 | ↓ |
Figure 3 Analysis of the metabolic pathway A: graph of the pathway analysis of the saline group versus the model group; B: graph of the pathway analysis of the Tuina group versus the model group. Each bubble in the bubble diagram represents a metabolic pathway. The bubble's horizontal coordinates and bubble size indicate the influence factor size of the pathway in the topological analysis; the vertical coordinates of the bubble and the bubble color indicate the P-value of the enrichment analysis.
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