Journal of Traditional Chinese Medicine ›› 2022, Vol. 42 ›› Issue (2): 227-233.DOI: 10.19852/j.cnki.jtcm.20220126.001
• Research Articles • Previous Articles Next Articles
WANG Wei1, LI Qingling1, MA Qiang2, XIA Ran2, GAO Bing2, WANG Yi3, WANG Jing4()
Received:
2021-07-22
Accepted:
2021-10-14
Online:
2022-01-26
Published:
2022-01-26
Contact:
WANG Jing
About author:
Prof. WANG Jing, Xin’an Medical Key Laboratory; Anhui University of Chinese Medicine, Hefei 230012, China. wangjing2161@126.com, Telephone: +86-13955189703Supported by:
WANG Wei, LI Qingling, MA Qiang, XIA Ran, GAO Bing, WANG Yi, WANG Jing. Effects of moxibustion at bilateral Feishu (BL13) and Xinshu (BL15) combined with benazepril on myocardial cells apoptosis index and apoptosis-related proteins cytochrome c and apoptosis-inducing factor in rats with chronic heart failure[J]. Journal of Traditional Chinese Medicine, 2022, 42(2): 227-233.
Figure 1 Model identification A: echocardiography in normal group; B: echocardiography in model-I group; C: EF between normal group and model-I group; D: FS between normal group and model-I group. Normal group: rats were fed normally (n = 3); Model-I group: CHF rats were fed normally without treatment (n = 3). aP < 0.01, compared with normal group.
Figure 2 Comparisons of echocardiogram indexes in different groups A-E, G-K: echocardiography in different groups; F: echocardiogram indexes in different groups; F1: EF in different groups; F2: FS in different groups; F3: SV in different groups; L: HR in different groups. Normal group: rats were fed normally without intervention (n = 10). Model group: CHF rats were fed normally without treatment (n = 6). Moxibustion group (abbreviate as Mox group in F1-F3, L): CHF rats were performed for 20 min at Feishu (BL13) and Xinshu (BL15) with moxa sticks daily for 3 weeks (n = 8); benazepril group (abbreviate as Ben group in F1-F3, L): CHF rats were administered with benazepril at a daily dose of 2 mg/kg for 3 weeks (n = 7); aibei group: CHF rats were administered with benazepril at a daily dose of 2 mg/kg, and performed for 20 min at Feishu (BL13) and Xinshu (BL15) with moxa sticks daily for 3 weeks (n = 9). aP < 0.01, compared with normal group; bP < 0.01, compared with model group; cP < 0.05, dP < 0.01, compared with Aibei group.
Group | n | CO (mL/ min) | LVSP (kPa) | LVEDP (kPa) |
---|---|---|---|---|
Normal | 10 | 34.867±1.420 | 23.683±1.170 | 0.581±0.020 |
Model | 6 | 20.143±1.301a | 12.431±1.230a | 1.682±0.060a |
Moxibustion | 8 | 28.373±0.670bd | 18.403±0.410bd | 1.083±0.032bd |
Benazepril | 7 | 25.627±2.710bd | 17.471±0.720bd | 1.123±0.031bd |
Aibei | 9 | 32.707±1.650b | 20.821±0.820b | 0.823±0.040b |
Table 1 Comparisons of hemodynamic indices in different groups ($\bar{x}±s$)
Group | n | CO (mL/ min) | LVSP (kPa) | LVEDP (kPa) |
---|---|---|---|---|
Normal | 10 | 34.867±1.420 | 23.683±1.170 | 0.581±0.020 |
Model | 6 | 20.143±1.301a | 12.431±1.230a | 1.682±0.060a |
Moxibustion | 8 | 28.373±0.670bd | 18.403±0.410bd | 1.083±0.032bd |
Benazepril | 7 | 25.627±2.710bd | 17.471±0.720bd | 1.123±0.031bd |
Aibei | 9 | 32.707±1.650b | 20.821±0.820b | 0.823±0.040b |
Figure 3 Comparisons of myocardial cells apoptosis in different groups A-E: TUNEL staining in different groups (×200); F: myocardial cells apoptosis index in different groups. Normal group: rats were fed normally without intervention (n = 10); model group: CHF rats were fed normally without treatment (n = 6); moxibustion group (abbreviate as Mox group in F): CHF rats were performed for 20 min at Feishu (BL13) and Xinshu (BL15) with moxa sticks daily for 3 weeks (n = 8); benazepril group (abbreviate as Ben group in F): CHF rats were administered with benazepril at a daily dose of 2 mg/kg for 3 weeks (n = 7); aibei group: CHF rats were administered with benazepril at a daily dose of 2 mg/kg, and performed for 20 min at Feishu (BL13) and Xinshu (BL15) with moxa sticks daily for 3 weeks (n = 9). TUNEL: terminal-deoxynucleoitidyl transferase mediated nick end labeling; CHF: chronic heart failure. aP < 0.01, compared with normal group; bP < 0.01, compared with model group; dP < 0.01, compared with Aibei group.
Figure 4 Pathological changes of left ventricular myocardial tissue in different groups A-E: HE staining in different groups (×400). → : indicates changes of myocardial cell swelling and cytoplasmic vacuolation; ←: indicates rupture and dissolution of myocardial fiber. Normal group: rats were fed normally without intervention (n = 10); model group: CHF rats were fed normally without treatment (n = 6); moxibustion group: CHF rats were performed for 20 min at Feishu (BL13) and Xinshu (BL15) with moxa sticks daily for 3 weeks (n = 8); benazepril group: CHF rats were administered with benazepril at a daily dose of 2 mg/kg for 3 weeks (n = 7); Aibei group: CHF rats were administered with benazepril at a daily dose of 2 mg/kg, and performed for 20 min at Feishu (BL13) and Xinshu (BL15) with moxa sticks daily for 3 weeks (n = 9). HE: hematoxylin and eosin; CHF: chronic heart failure.
Figure 5 Comparisons of the expressions of Cyt-C and AIF in different groups A: Cyt-C, AIF and GAPHD protein expression in different groups; 1: normal group; 2: model group; 3: moxibustion group; 4: benazepril group; 5: aibei group; B: the expression level of Cyt-C in different groups; C: the expression level of AIF in different groups. Normal group: rats were fed normally without intervention (n = 10); model group: CHF rats were fed normally without treatment (n = 6); moxibustion group (abbreviate as Mox group in B, C): CHF rats were performed for 20 min at Feishu (BL13) and Xinshu (BL15) with moxa sticks daily for 3 weeks (n = 8); benazepril group (abbreviate as Ben group in B, C): CHF rats were administered with benazepril at a daily dose of 2 mg/kg for 3 weeks (n = 7); Aibei group: CHF rats were administered with benazepril at a daily dose of 2 mg/kg, and performed for 20 min at Feishu (BL13) and Xinshu (BL15) with moxa sticks daily for 3 weeks (n = 9). Cyt-C: cytochrome c; AIF: apoptosis-inducing factor; GAPHD: glyceraldehyde-3-phosphate dehydrogenase; CHF: chronic heart failure. aP < 0.01, compared with normal group; bP < 0.01, compared with model group; cP < 0.05, dP < 0.01, compared with Aibei group.
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