Journal of Traditional Chinese Medicine ›› 2024, Vol. 44 ›› Issue (4): 784-793.DOI: 10.19852/j.cnki.jtcm.20240610.005
• Original articles • Previous Articles Next Articles
HU Yuanyuan1, LIU Xinguang2,3, ZHAO Peng2,3, WU Jinyan2,3, YAN Xinhua2,3, HOU Runsu2,3, WANG Xiangcheng2,3, YANG Fan2,3, TIAN Xinrong2,3, LI Jiansheng2,3()
Received:
2022-11-22
Accepted:
2023-04-28
Online:
2024-08-15
Published:
2024-06-10
Contact:
LI Jiansheng, Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan and Education Ministry of China, Zhengzhou 450046, China; Henan Key Laboratory of Chinese Medicine for Respiratory Disease, Henan University of Chinese Medicine, Zhengzhou 450046, China. Supported by:
HU Yuanyuan, LIU Xinguang, ZHAO Peng, WU Jinyan, YAN Xinhua, HOU Runsu, WANG Xiangcheng, YANG Fan, TIAN Xinrong, LI Jiansheng. Integration of serum pharmacochemistry with network pharmacology to reveal the potential mechanism of Yangqing Chenfei formula (养清尘肺方) for the treatment of silicosis[J]. Journal of Traditional Chinese Medicine, 2024, 44(4): 784-793.
No. | Component | Formula | RT | m/z |
---|---|---|---|---|
1 | Arginine | C6H14N4O2 | 1.28 | 175.12 |
2 | Aspartic acid | C4H7NO4 | 1.32 | 134.04 |
3 | Baicalin | C21H18O11 | 21.71 | 447.09 |
4 | Eriocitrin | C27H32O15 | 16.98 | 595.17 |
5 | Esculin | C15H16O9 | 13.39 | 339.07 |
6 | Gallic acid | C7H6O5 | 4.17 | 169.01 |
7 | Ginsenoside Rb1 | C54H92O23 | 26.34 | 1109.61 |
8 | L-Malic acid | C4H6O5 | 1.64 | 133.01 |
9 | Narirutin | C27H32O14 | 18.53 | 581.19 |
10 | Nobiletin | C21H22O8 | 39.61 | 403.14 |
11 | Oxypaeoniflorin | C23H28O12 | 13.97 | 495.15 |
12 | Paeoniflorin | C23H28O11 | 15.83 | 479.16 |
13 | Paeonol | C9H10O3 | 28.07 | 167.07 |
14 | Peimine | C27H45NO3 | 20.61 | 432.35 |
15 | Peiminine | C27H43NO3 | 22.09 | 430.33 |
16 | Rutin | C27H30O16 | 16.88 | 609.15 |
17 | Scopoletin | C10H8O4 | 18.34 | 193.05 |
18 | Tangeretin | C20H20O7 | 43.03 | 373.13 |
19 | Threonine | C4H9NO3 | 1.32 | 120.07 |
Table 1 Information of the 19 active components in YCF medicated-serum
No. | Component | Formula | RT | m/z |
---|---|---|---|---|
1 | Arginine | C6H14N4O2 | 1.28 | 175.12 |
2 | Aspartic acid | C4H7NO4 | 1.32 | 134.04 |
3 | Baicalin | C21H18O11 | 21.71 | 447.09 |
4 | Eriocitrin | C27H32O15 | 16.98 | 595.17 |
5 | Esculin | C15H16O9 | 13.39 | 339.07 |
6 | Gallic acid | C7H6O5 | 4.17 | 169.01 |
7 | Ginsenoside Rb1 | C54H92O23 | 26.34 | 1109.61 |
8 | L-Malic acid | C4H6O5 | 1.64 | 133.01 |
9 | Narirutin | C27H32O14 | 18.53 | 581.19 |
10 | Nobiletin | C21H22O8 | 39.61 | 403.14 |
11 | Oxypaeoniflorin | C23H28O12 | 13.97 | 495.15 |
12 | Paeoniflorin | C23H28O11 | 15.83 | 479.16 |
13 | Paeonol | C9H10O3 | 28.07 | 167.07 |
14 | Peimine | C27H45NO3 | 20.61 | 432.35 |
15 | Peiminine | C27H43NO3 | 22.09 | 430.33 |
16 | Rutin | C27H30O16 | 16.88 | 609.15 |
17 | Scopoletin | C10H8O4 | 18.34 | 193.05 |
18 | Tangeretin | C20H20O7 | 43.03 | 373.13 |
19 | Threonine | C4H9NO3 | 1.32 | 120.07 |
Figure 1 Functional analysis of the active component targets A: top 20 pathways enriched by the top 100 active component targets; B: top 10 biological processes enriched by the top 100 active component targets. The larger the pvalue, the redder the color. The size of the node is proportional to its count. KEGG: Kyoto Encyclopedia of Genes and Genomes.
Figure 2 Functional analysis of the silicosis genes A: top 20 pathways enriched by the top 100 silicosis genes; B: top 10 biological processes enriched by the top 100 silicosis genes. The larger the pvalue, the redder the color. The size of the node is proportional to its count. KEGG: Kyoto Encyclopedia of Genes and Genomes.
Figure 3 Intersection analysis of active component targets and silicosis genes A: herb-component-gene-pathway network; B: biological process analysis of 61 common genes. Rhombus represents herb, round represents active component, hexagon represents gene, arrow represents pathway. The size of the node is proportional to its degree. YCF: Yangqing Chenfei formula; ZW: Ziwan (Radix Asteris Tatarici); JG: Jiegeng (Radix Platycodi); GL: Gualou (Fructus et Semen Trichosanthis); ZB: Zhebeimu (Bulbus Fritillariae Thunbergii); XYS: Xiyangshen (Radix Panacis Quinquefolii); CS: Chishao (Radix Paeoniae Rubra); CP: Chenpi (Pericarpium Citri Reticulatae).
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