Journal of Traditional Chinese Medicine ›› 2023, Vol. 43 ›› Issue (5): 1026-1033.DOI: 10.19852/j.cnki.jtcm.2023.05.006
Previous Articles Next Articles
JIANG Wenjing1, JIANG Huaying2,3, YUAN Lihua4, SA Yuanhong5, XIAO Jimei6, SUN Hongqi7, SONG Jingyan8,9(), SUN Zhengao8,9()
Received:
2022-05-23
Accepted:
2022-08-12
Online:
2023-10-15
Published:
2023-08-29
Contact:
Prof. SONG Jingyan, Reproductive Medicine Center of Integration of Traditional and Western Medicine, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China. Supported by:
JIANG Wenjing, JIANG Huaying, YUAN Lihua, SA Yuanhong, XIAO Jimei, SUN Hongqi, SONG Jingyan, SUN Zhengao. Xiaoyi Yusi decoction (消异育嗣汤) improves in vitro fertilization and embryo transfer outcomes in patients with endometriosis[J]. Journal of Traditional Chinese Medicine, 2023, 43(5): 1026-1033.
Item | Patients group (n = 28) | Treatment group (n = 32) | Control group (n = 33) |
---|---|---|---|
Age (years) | 30.3±4.1 | 30.8±3.5 | 29.6±3.6 |
Infertility years (years) | 3.4±1.9 | 3.1±2.0 | 2.2±1.5 |
Primary infertility (n) | 17 | 16 | 15 |
Secondary infertility (n) | 11 | 16 | 18 |
Basic FSH (mIU/mL) | 7.4±3.8 | 7.5±3.3 | 7.0±2.5 |
Basic LH (mIU/mL) | 4.6±1.7 | 4.9±2.7 | 4.9±1.7 |
Basic E2 (pg/mL) | 48.5±29.2 | 49.9±26.8 | 45.5±27.5 |
Table 1 Clinical background of subjects
Item | Patients group (n = 28) | Treatment group (n = 32) | Control group (n = 33) |
---|---|---|---|
Age (years) | 30.3±4.1 | 30.8±3.5 | 29.6±3.6 |
Infertility years (years) | 3.4±1.9 | 3.1±2.0 | 2.2±1.5 |
Primary infertility (n) | 17 | 16 | 15 |
Secondary infertility (n) | 11 | 16 | 18 |
Basic FSH (mIU/mL) | 7.4±3.8 | 7.5±3.3 | 7.0±2.5 |
Basic LH (mIU/mL) | 4.6±1.7 | 4.9±2.7 | 4.9±1.7 |
Basic E2 (pg/mL) | 48.5±29.2 | 49.9±26.8 | 45.5±27.5 |
Group | n | Total integral (before treatment) | Total integral (after treatment) | Integral difference | P value |
---|---|---|---|---|---|
Patients | 28 | 11.4±1.8 | 11.1±1.7 | 0.29±1.03 | > 0.05 |
Treatment | 32 | 11.8±1.7 | 8.7±2.0a | 3.06±1.73a | < 0.01 |
Table 2 Total integral of the patient group and the treatment group before and after the experiment
Group | n | Total integral (before treatment) | Total integral (after treatment) | Integral difference | P value |
---|---|---|---|---|---|
Patients | 28 | 11.4±1.8 | 11.1±1.7 | 0.29±1.03 | > 0.05 |
Treatment | 32 | 11.8±1.7 | 8.7±2.0a | 3.06±1.73a | < 0.01 |
Item | Patients group (n = 28) | Treatment group (n = 32) | Control group (n = 33) | |
---|---|---|---|---|
Number of oocyte (n) | 8.9±6.5 | 12.1±6.6a | 14.1±5.0a | |
Number of embryos (n) | 2.1±1.8 | 3.1±2.4a | 4.4±2.1a | |
Rate of high-quality egg (%) | 45.4 | 65.0a | 62.2a | |
Rate of high-quality embryos (%) | 33.3 | 51.2b | 51.3b | |
Biochemical pregnancy rate (%) | 75.0 | 81.3 | 87.9 | |
Clinical pregnancy rate (%) | 57.1 | 71.9a | 87.9a |
Table 3 The number of follicles and endocrine parameters during the day of HCG
Item | Patients group (n = 28) | Treatment group (n = 32) | Control group (n = 33) | |
---|---|---|---|---|
Number of oocyte (n) | 8.9±6.5 | 12.1±6.6a | 14.1±5.0a | |
Number of embryos (n) | 2.1±1.8 | 3.1±2.4a | 4.4±2.1a | |
Rate of high-quality egg (%) | 45.4 | 65.0a | 62.2a | |
Rate of high-quality embryos (%) | 33.3 | 51.2b | 51.3b | |
Biochemical pregnancy rate (%) | 75.0 | 81.3 | 87.9 | |
Clinical pregnancy rate (%) | 57.1 | 71.9a | 87.9a |
Figure 2 Heat map from the data of significantly differential metabolites The horizontal axis of the figure shows a dendrogram of the samples. The vertical axis of the figure shows a dendrogram of the metabolite differences.
Figure 3 VIP scores of each potential biomarker to the discrimination between the patient group and the treatment group The contribution of every potential biomarker to the discrimination between the patient group and the treatment group were ranked as VIP scores. VIP: variable importance in projection.
Figure 4 Metabolic pathway analysis of significantly changed metabolites between the patient group and the treatment group The results showed that pyruvate and glycerophospholipid metabolism pathways and the citrate cycle are closely related to endometriosis, indicating that they may be targetted in the treatment of endometriosis with XYYSD. XYYSD: Xiaoyi Yusi decoction.
1. |
Calagna G, Della Corte L, Giampaolino P, Maranto M, Perino A. Endometriosis and strategies of fertility preservation: a systematic review of the literature. Eur J Obstet Gynecol Reprod Biol 2020; 254: 218-25.
DOI PMID |
2. |
Burns WN, Schenken RS. Pathophysiology of endometriosis-associated infertility. Clin Obstet Gynecol 1999; 42: 586-610.
PMID |
3. | Li X, Zeng C, Zhou YF, et al. Endometriosis fertility index for predicting pregnancy after endometriosis surgery. Chin Med J (Engl) 2017; 130: 1932-7. |
4. | Wang WJ, Yang YH, Lu ZL. Observation on the therapeutic effect of tonifying kidney and removing blood stasis on infertility caused by endometriosis by stages. Zhong Guo Min Zu Min Jian Yi Yao 2018; 27: 83-4. |
5. |
Aderemi AV, Ayeleso AO, Oyedapo OO, Mukwevho E. Metabolomics: a scoping review of its role as a tool for disease biomarker discovery in selected non-communicable diseases. Metabolites 2021; 11: 418.
DOI URL |
6. |
Sinha R, Sharma B, Dangi AK, Shukla P. Recent metabolomics and gene editing approaches for synthesis of microbial secondary metabolites for drug discovery and development. World J Microbiol Biotechnol 2019; 35: 166.
DOI |
7. |
Wang M, Huang J, Fan H, et al. Treatment of rheumatoid arthritis using combination of methotrexate and tripterygium glycosides tablets-A quantitative plasma pharmacochemical and pseudo-targeted metabolomic approach. Front Pharmacol 2018; 9: 1051.
DOI URL |
8. |
Hou Y, He D, Ye L, Wang G, Zheng Q, Hao H. An improved detection and identification strategy for untargeted metabolomics based on UPLC-MS. J Pharm Biomed Anal 2020; 191: 113531.
DOI URL |
9. |
Nyamundanda G, Gormley IC, Fan Y, Gallagher WM, Brennan L. MetSizeR: selecting the optimal sample size for metabolomic studies using an analysis based approach. BMC Bioinformatics 2013; 14: 338.
DOI PMID |
10. | State Administration of Traditional Chinese Medicine. Criteria of diagnosis and therapeutic effect of diseases and syndromes in Traditional Chinese Medicine. Nanjing: Nanjing University Press, 1994: 204-5. |
11. | Luo SP, Liu YF. Gynecology of Traditional Chinese Medicine. 3rd ed. Beijing: People’s Medical Publishing House, 2017: 283. |
12. |
Song JY, Wang XM, Guo Y, et al. Novel high-coverage targeted metabolomics method (SWATHtoMRM) for exploring follicular fluid metabolome alterations in women with recurrent spontaneous abortion undergoing in vitro fertilization. Sci Rep 2019; 9: 10873.
DOI |
13. | Sun ZG, Song JY, Zhang XX, et al. Novel SWATHTM technology for follicular fluid metabolomics in patients with endometriosis. Pharmazie 2018; 73: 318-23. |
14. |
Chong J, Soufan O, Li C, et al. MetaboAnalyst 4.0: towards more transparent and integrative metabolomics analysis. Nucleic Acids Res 2018; 46: W486-94.
DOI URL |
15. |
Yang F, Du J, Zhang H, et al. Serum metabolomics of burkitt lymphoma mouse models. Plos One 2017; 12: e0170896.
DOI URL |
16. | Chong J, Wishart DS, Xia J. Using metaboAnalyst 4.0 for comprehensive and integrative metabolomics data analysis. Curr Protoc Bioinformatics 2019; 68: e86. |
17. |
Zhao RH. Strategies for activating blood circulation-regulating gan (liver)-tonifying shen (kidney) sequential therapy of endometriosis-associated infertility. Chin J Integr Med 2019; 25: 243-5.
DOI |
18. | Song JY, Sun ZG, Zhang XX, Wang AJ. Preliminary screening of metabolites of follicular fluid in endometriosis-related infertility and its relationship with oocyte quality. Zhong Hua Sheng Zhi Yu Bi Yun Za Zhi 2017; 37: 519-27. |
19. |
Moreira F, Corcini CD, Mondadori RG, et al. Leptin and mitogen-activated protein kinase (MAPK) in oocytes of sows and gilts. Anim Reprod Sci 2013; 139: 89-94.
DOI PMID |
20. |
Gao J, Xu K, Liu H, et al. Impact of the gut microbiota on intestinal immunity mediated by tryptophan metabolism. Front Cell Infect Microbiol 2018; 8: 13.
DOI URL |
21. |
Santos T, Carvalho J, Corvo MC, Cabrita EJ, Queiroz JA, Cruz C. L-tryptophan and dipeptide derivatives for supercoiled plasmid DNA purification. Int J Biol Macromol 2016; 87: 385-96.
DOI PMID |
22. |
Chen M, Zhang B, Cai S, et al. Metabolic disorder of amino acids, fatty acids and purines reflects the decreases in oocyte quality and potential in sows. J Proteomics 2019; 200: 134-43.
DOI PMID |
23. |
de Bruin JP, Bovenhuis H, van Noord PA, et al. The role of genetic factors in age at natural menopause. Hum Reprod 2001; 16: 2014-8.
PMID |
24. | Zhang GG, Zhao LF, Zhu JC, Feng YF, Wu X. Anti-inflammatory activities and glycerophospholipids metabolism in KLA-stimulated RAW 264.7 macrophage cells by diarylheptanoids from the rhizomes of alpinia officinarum. Biomed Chromatogr 2018; 32. |
25. |
Li JJ, Gao Y, Guan LH, et al. Discovery of phosphatidic acid, phosphatidylcholine, and phosphatidylserine as biomarkers for early diagnosis of endometriosis. Front Physiol 2018; 9: 14.
DOI PMID |
26. |
Preis KA, Seidel GJ, Gardner DK. Metabolic markers of developmental competence for in vitro-matured mouse oocytes. Reproduction 2005; 130: 475-83.
DOI URL |
27. |
Peralta OA, Bucher D, Angulo C, Castro MA, Ratto MH, Concha I. Tissue localization of GM-CSF receptor in bovine ovarian follicles and its role on glucose uptake by mural granulosa cells. Anim Reprod Sci 2016; 170: 157-69.
DOI PMID |
28. |
Wang L, Tang JH, Wang L, et al. Oxidative stress in oocyte aging and female reproduction. J Cell Physiol 2021; 236: 7966-83.
DOI PMID |
29. |
Mori T, Ito F, Koshiba A, et al. Aromatase as a target for treating endometriosis. J Obstet Gynaecol Res 2018; 44: 1673-81.
DOI URL |
30. |
Rocha-Junior CV, Da Broi MG, Miranda-Furtado CL, Navarro PA, Ferriani RA, Meola J. Progesterone receptor B (PGR-B) is partially methylated in eutopic endometrium from infertile women with endometriosis. Reprod Sci 2019; 26: 1568-74.
DOI PMID |
31. | Vogel M, Träger L. Purine nucleoside triphosphates in streptomyces hydrogenans. Influence of steroids on the relative nucleotide level. Zentralbl Bakteriol Orig A 1979; 245: 130-8. |
32. |
Ren J, Lakoski S, Haller RG, Sherry AD, Malloy CR. Dynamic monitoring of carnitine and acetylcarnitine in the trimethylamine signal after exercise in human skeletal muscle by 7T 1H-MRS. Magn Reson Med 2013; 69: 7-17.
DOI PMID |
33. |
Fabara AN, Fraaije MW. Production of indigo through the use of a dual-function substrate and a bifunctional fusion enzyme. Enzyme Microb Technol 2020; 142: 109692.
DOI URL |
34. |
Pazos M, Andersen ML, Skibsted LH. Amino acid and protein scavenging of radicals generated by iron/hydroperoxide system: an electron spin resonance spin trapping study. J Agric Food Chem 2006; 54: 10215-21.
DOI URL |
35. |
Chimerel C, Field CM, Piñero-Fernandez S, Keyser UF, Summers DK. Indole prevents escherichia coli cell division by modulating membrane potential. Biochim Biophys Acta 2012; 1818: 1590-4.
DOI PMID |
36. |
Field CM, Summers DK. Indole inhibition of ColE1 replication contributes to stable plasmid maintenance. Plasmid 2012; 67: 88-94.
DOI PMID |
37. |
Matchett WH. Inhibition of tryptophan synthetase by indoleacrylic acid. J Bacteriol 1972; 110: 146-54.
DOI PMID |
38. |
Kuang Y, Han XY, Xu M, Wang Y, Zhao YX, Yang Q. Oxaloacetate ameliorates chemical liver injury via oxidative stress reduction and enhancement of bioenergetic fluxes. Int J Mol Sci 2018; 19: 1626-40.
DOI URL |
[1] | LI Chaoran, YANG Yan, FENG Chuwen, LI Heng, QU Yuanyuan, WANG Yulin, WANG Delong, WANG Qingyong, GUO Jing, SHI Tianyu, SUN Xiaowei, WANG Xue, HOU Yunlong, SUN Zhongren, YANG Tiansong. Integrated 'omics analysis for the gut microbiota response to moxibustion in a rat model of chronic fatigue syndrome [J]. Journal of Traditional Chinese Medicine, 2023, 43(6): 1176-1189. |
[2] | WANG Miao, ZHU Yan, ZHAO Hui, ZHAO Hongfang. Moxibustion enables protective effects on rheumatoid arthritis-induced myocardial injury via transforming growth factor beta1 signaling and metabolic reprogramming [J]. Journal of Traditional Chinese Medicine, 2023, 43(6): 1190-1199. |
[3] | WANG Chao, WU Qiong, LI Ping, WANG Zhigang, LOU Xusheng, LI Yuanyuan, ZHANG Lin. Effect of Traditional Chinese Medicine combined with Western Medicine on blood lipid levels and inflammatory factors in patients with angina pectoris in coronary heart disease identified as intermingled phlegm and blood stasis syndrome: a network Meta-analysis [J]. Journal of Traditional Chinese Medicine, 2023, 43(4): 640-649. |
[4] | LIU Di, ZHANG Yingqi, YU Tianyuan, LIU Zhifeng, JIAO Yi, WANG Hourong, XU Yajing, GUAN Qian, CHEN Lulu, HU Hui. Protective mechanisms of Tuina therapy against lipopolysaccharide-induced fever in young rabbits based on untargeted metabolomics analysis [J]. Journal of Traditional Chinese Medicine, 2023, 43(4): 725-733. |
[5] | LUO Xin, XIE Jing, HUANG Li, GAN Wenfan, CHEN Ming. Efficacy and safety of activating blood circulation and removing blood stasis of Traditional Chinese Medicine for managing renal fibrosis in patients with chronic kidney disease: a systematic review and Meta-analysis [J]. Journal of Traditional Chinese Medicine, 2023, 43(3): 429-440. |
[6] | ZHAO Qingyi, SUN Yi, ZHOU Jing, GAO Yanling, MA Guizhi, HU Zhihai, WANG Yi, SHI Yin. Effectiveness of herb-partitioned moxibustion combined with electroacupuncture on polycystic ovary syndrome in patients with symptom pattern of kidney deficiency and phlegm-dampness [J]. Journal of Traditional Chinese Medicine, 2021, 41(6): 985-993. |
[7] | HONG Zongchao, CAI Quan, DUAN Xueyun, YANG Yanfang, WU Hezhen, JIANG Nan, FAN Heng. Effect of compound Sophorae decoction in the treatment of ulcerative colitis by tissue extract metabolomics approach [J]. Journal of Traditional Chinese Medicine, 2021, 41(3): 414-423. |
[8] | YAO Zouying;WANG Jing;LU Hua;LI Limin;ZHUO Tianxiu;WAN Qian;YIE Shangmian;. Zichong granules(资冲颗粒)promote differentiation of ovarian granulosa-like cells from human embryonic stem cells in vitro [J]. Journal of Traditional Chinese Medicine, 2021, 41(2): 203-211. |
[9] | Guo Xinwei, Lang Na, Li Guanru, Li Ping, Qu Jianhua, Zhou Dongmei, Wang Ping, Cang Zhang, Deng BingXu, Lou Weihai, Xu Jingna, Lan Haibing, Liu Yumin, Zhang Weishu, Sun Liyun. Effectiveness of flesh-moistening paste in psoriasis vulgaris patients with symptom pattern of blood stasis: a randomized and parallel-controlled trial [J]. Journal of Traditional Chinese Medicine, 2020, 40(6): 1018-1025. |
[10] | Liu Mi, Shen Jiacheng, Liu Caichun, Zhong Huan, Yang Qing, Shu Wenna, Ma Mingzhu, Dong Jiyang, Yang Zongbao, Chang Xiaorong, She Chang, Yu Shu. Effects of moxibustion and acupuncture at Zusanli(ST 36) and Zhongwan(CV 12) on chronic atrophic gastritis in rats [J]. Journal of Traditional Chinese Medicine, 2020, 40(5): 827-835. |
[11] | Mao Shuai, Zuo Tian, Jiang Lu, Zhang Minzhou. Effect of supplementing Qi and promoting blood circulation therapy on left ventricular remodeling: a systematic review and Meta-analysis [J]. Journal of Traditional Chinese Medicine, 2020, 40(3): 365-375. |
[12] | Hu Hantong, Chen Lifang, Jin Xiaofei, Li Ru, Fang Jianqiao. Effect of herb-partitioned moxibustion on pain and quality of life in women with endometriosis: a protocol for a randomized clinical trial [J]. Journal of Traditional Chinese Medicine, 2020, 40(2): 324-332. |
[13] | Qu Tiange, Mei Chencheng, Zeng Yajun, Li Lingling, Duan Xingwu. Correlation analysis of Treg/Th17 cells and related cytokines in patients with psoriasis vulgaris [J]. Journal of Traditional Chinese Medicine, 2019, 39(05): 700-706. |
[14] | Zhang Zhijie, Liu Shaoming, Zhang Yueyang, Yang Jingzhe, Kong Tao, Wang Chengli, Ning Peng, Chen Xiaochao, Li Xuesong, Jia Yusen, Chen Xiaojun. Traditional Chinese Medicine syndrome elements of male infertility revealed by latent tree model analysis [J]. Journal of Traditional Chinese Medicine, 2018, 38(06): 926-935. |
[15] | Tan Yong, Shen Shanshan, Guan Fulan, He Bing, Lu Cheng, Xiao Cheng, Jiang Miao, Zhao Ning, Li Li, Cheng Shiping, Zu Xianpeng, Zhang Weidong, Liu Xinru, Lü Aiping. Exploring pathogenesis in subjects with subjective Tinnitus having kidney deficiency pattern in terms of Traditional Chinese Medicine based on serum metabolic profiles [J]. Journal of Traditional Chinese Medicine, 2018, 38(05): 773-780. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||
Sponsored by China Association of Chinese Medicine
& China Academy of Chinese Medical Sciences
16 Nanxiaojie, Dongzhimen Nei, Beijing, China. 100700 Email: jtcmen@126.com
Copyright 2020 Journal of Traditional Chinese Medicine. All rights reserved.