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Journal of Traditional Chinese Medicine ›› 2021, Vol. 41 ›› Issue (1): 68-78.DOI: 10.19852/j.cnki.jtcm.2021.01.009

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Qingre Jianpi decoction(清热健脾汤) attenuates inflammatory responses by suppressing NOD-like receptor family pyrin domain-containing 3 inflammasome activation in dextran sulfate sodium-induced colitis mice

ZHANG Jiayan, KANG Xiuhong, SUN Mengyun, ZHANG Shengsheng   

  1. Department of Digestive System, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University;
  • Online:2021-02-03 Published:2020-12-09
  • Supported by:
    Supported by National Science Foundation program of China (Activation of colonic mucosal mast cells and ion transport associated with serotonin receptors in the study of the role of soothing the liver and strengthening the spleen in the treatment of diarrheal irritable bowel syndrome, No.81473644)

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Abstract: OBJECTIVE: To investigate the effects of Qingre Jianpi decoction(清热健脾汤,QRJPD) on dextran sulfate sodium(DSS)-induced colitis mice and explore its mechanism.METHODS: All mice were randomly divided into six groups. Weight changes, disease activity index values, and histological damage were detected. Inflammatory cytokines and immune cell infiltration were measured using enzyme-linked immunosorbent assays(ELISA) and immunohistochemistry(IHC) method. The key protein expression levels of the NOD-like receptor family pyrin domain-containing 3(NLRP3) inflammasome were detected by western blot analysis, IHC, and quantitative reverse transcription polymerase chain reaction.RESULTS: QRJPD played an anti-inflammatory role in the treatment of ulcerative colitis(UC), reduced the secretion of inflammatory cytokines, and inhibited the inflammatory infiltration of immune cells by suppressing DSS-induced activation of the NLRP3 inflammasome.CONCLUSION: QRJPD exerts protective effects by inhibiting DSS-induced NLRP3 inflammasome activation.

Key words: Colitis,ulcerative, anti-inflammatory agents, NLR family,pyrin domain-containing 3 protein, inflammasomes

Cite this article

ZHANG Jiayan, KANG Xiuhong, SUN Mengyun, ZHANG Shengsheng. Qingre Jianpi decoction(清热健脾汤) attenuates inflammatory responses by suppressing NOD-like receptor family pyrin domain-containing 3 inflammasome activation in dextran sulfate sodium-induced colitis mice[J]. Journal of Traditional Chinese Medicine, 2021, 41(1): 68-78.