Phytochemical analysis, antioxidant, acetylcholinesterase, and α-amylase inhibitors from Hippeastrum hybridum extract

doi:10.19852/j.cnki.jtcm.2024.03.001

Abstract

Abstract:

OBJECTIVE: To investigate the effects of Hippeastrum hybridum (HH) as a free radical scavenger, and an inhibitor of the two enzymes i-e Alpha-amylase (α-amylase) and acetylcholinesterase (AChE).

METHODS: In this study, HH plant was preliminary analyzed for phytochemical screening and then tested for its antioxidant, anti-α-amylase, and anti-AChE efficiency via standard procedures.

RESULTS: Phytochemical analysis shows the existence of different compounds; while Coumarins and quinones were absent. The total phenolic, flavonoid, and tannins content were found to be (78.52 ± 0.69) mg GAE/g, (2.01 ± 0.04) mg RUE/g, and (58.12 ± 0.23) mg TAE/g of plant extract respectively. 28.02% ± 0.02% alkaloid and 2.02% ± 0.05% saponins were present in the HH extract. The HH extract showed the anti-oxidant property with IC50 (50% inhibition) of (151.01 ± 0.13) (HH), (79.01 ± 0.04) (Ascorbic acid) for ferric reducing, (91.48 ± 0.13) (HH), (48.02 ± 0.11) (Ascorbic acid) against Ammonium molybdenum, (156.02 ± 0.31) (HH), (52.38 ± 0.21) (Ascorbic acid) against DPPH, 136.01 ± 0.21 (HH), 52.02± 0.31 (Ascorbic acid) against H₂O₂, and 154.12 ± 0.03 (HH), (40.05 ± 0.15) (Ascorbic acid) μg/mL against ABTS respectively. Statistical analysis indicated that HH caused a competitive type of inhibition of α-amylase (V_max remained constant and Km increases from 10.65 to 84.37%) while Glucophage caused the un-competitive type of inhibition i-e both Km and V_max decreased from 40.49 to 69.15% and 38.86 to 69.61% respectively. The K_i, (inhibition constant); K_I, (dissociation constant), K_m, (Michaelis-Menten constant), and IC50 were found to be 62, 364, 68.1, and 38.08 ± 0.22 for HH and 12, 101.05, 195, 34.01 ± 0.21 for Glucophage. Similarly, HH causes an anon-competitive type of inhibition of AChE i-e Km remains constant while V_max decreases from 60.5% to 74.1%. The calculated K_i, K_I, K_m, and IC50 were found to be 32, 36.2, 0.05, and 18.117 ± 0.018.

CONCLUSION: From the current results, it is concluded that HH extract contains bioactive compounds, and could be a good alternative to controlling oxidants, Alzheimer's and Type-II diabetic diseases.

Key words: Hippeastrum hybridum, phytochemicals, antioxidant, alpha-amylase, acetylcholinesterase

Naila Sher, Mushtaq Ahmed, Nadia Mushtaq. Phytochemical analysis, antioxidant, acetylcholinesterase, and α-amylase inhibitors from Hippeastrum hybridum extract[J]. Journal of Traditional Chinese Medicine, 2024, 44(3): 496-504.

Figures/Tables 1

References 65.

1.	Ahmed M, Sher N, Mushtaq N, Khan RA. Phytochemical analysis and inhibitory effects of Calligonum polygonoides on pancreatic α-amylase and β-glucosidase enzymes. J Tradit Chin Med 2022; 42: 426.
2.	Emerit J, Edeas M, Bricaire F. Neurodegenerative diseases and oxidative stress. Biomed Pharmacother 2004; 58: 39-46. DOI PMID
3.	Kiss B, Popa DS, Crişan G, Bojiţǎ M, Loghin F. The evaluation of antioxidant potential of Veronica officinalis and Rosmarinus officinalis extracts by monitoring malondialdehide and glutathione levels in rats. Farmacia 2009; 57: 432-41.
4.	Shah A, Ahmed A, Sher N, et al. Efficacy of Silene arenosa extract on acetylcholinesterase in Bungarus sindanus (krait) venom. J Tradit Chin Med 2021; 41: 349-54.
5.	Thilagam E, Parimaladevi B, Kumarappan C, Chandra Mandal S. α-glucosidase and α-amylase inhibitory activity of senna surattensis. J Acupunct Meridian Stud 2013; 6: 24-30.
6.	Gupta R, Gigras P, Mohapatra H, Goswami VK, Chauhan B. Microbial α-amylases: a biotechnological perspective. Process Biochem 2003; 38 1599-616. DOI URL
7.	Fred-Jaiyesimi A, Kio A, Richard W. α-Amylase inhibitory effect of 3β-olean-12-en-3-yl (9Z)- hexadec-9-enoate isolated from Spondias mombin leaf. Food Chem 2009; 116: 285-88. DOI URL
8.	Singh SK, Rai PK, Jaiswal D, Watal G. Evidence-based critical evaluation of glycemic potential of cynodon dactylon. Evid Based Complement Alternat Med 2008; 5: 415-20. DOI URL
9.	Eruygur N, Icar E. Cholinesterase, α-glucosidase, α-amylase, and tyrosinase inhibitory effects and antioxidant activity of veronica officinalis extracts. Turk J Agr Eng Res 2018; 5: 253-25.
10.	Amor S, Puentes F, Baker D, van der Valk P. Inflammation in neurodegenerative diseases. Immunology 2010; 129: 154-69. DOI PMID
11.	Chen H, Kwong JC, Copes R, et al. Living near major roads and the incidence of dementia, Parkinson's disease, and multiple sclerosis: a population-based cohort study. Lancet 2017; 389 (10070): 718-26. DOI PMID
12.	Tan CC, Yu JT, Tan MS, Jiang T, Zhu XC, Tan L. Autophagy in aging and neurodegenerative diseases: implications for pathogenesis and therapy. Neurobiol Aging 2014; 35: 941-57. DOI URL
13.	Johri A, Beal MF. Mitochondrial dysfunction in neurodegenerative diseases. J Pharmacol Exp Ther 2012; 342: 619-30. DOI PMID
14.	Takalo M, Salminen A, Soininen H, Hiltunen M, Haapasalo A. Protein aggregation and degradation mechanisms in neurodegenerative diseases. Am J Neurodegener Dis 2013; 2: 1-14.
15.	Chen WW, Zhang X, Huang WJ. Role of neuroinflammation in neurodegenerative diseases (Review). Mol Med Rep 2016; 13: 3391-6. DOI URL
16.	Schliebs R, Arendt T. The cholinergic system in aging and neuronal degeneration. Behav Brain Res 2011; 221: 555-63. DOI PMID
17.	Butterfield DA, Swomley AM, Sultana R. Amyloid -peptide (1-42)-induced oxidative stress in alzheimer disease: importance in disease pathogenesis and progression. Antioxid Redox Signal 2013; 19: 823-35. DOI URL
18.	Hebert DN, Foellmer B, Helenius A. Glucose trimming and reglucosylation determine glycoprotein association with calnexin in the endoplasmic reticulum. Cell 1995; 81: 425-33. PMID
19.	Racchi M, Mazzucchelli M, Porrello E, Lanni C, Govoni S. Acetylcholinesterase inhibitors: novel activities of old molecules. Pharmacol Res 2004; 50: 441-51. PMID
20.	Kumar A, Singh A, Ekavali. A review on Alzheimer's disease pathophysiology and its management: an update. Pharmacol Rep 2015; 67: 195-203. DOI PMID
21.	Bores GM, Huger FP, Petko W, et al. Pharmacological evaluation of novel Alzheimer's disease therapeutics: acetylcholinesterase inhibitors related to galanthamine. J Pharmacol Exp Ther 1996; 277: 728‐38. PMID
22.	Gwaltney-Brant SM. Christmastime plants. Process Biochem 2006; 21: 643-63.
23.	Prasad KS, Savithramma N. Biosynthesis and Validation of Silver Nanoparticles from Nymphaea caerulea. Am J Adv Drug Deliv 2015 3149-159.
24.	Sofowora A. Medicinal plants and traditional medicine in Africa. Ibadan [[u.a.]: Spectrum Books Ltd., 1993: 32.
25.	Harborne JB. Phytochemical methods: a guide to modern techniques of plant analysis. London: Chapman and Hall, 1973: 622.
26.	Trease GE, Evans MD. A textbook of pharmacognosy. 13th ed. London: Builler Tindall and Caussel, 1989: 99.
27.	Siddiqui S, Verma A, Rather AA, Jabeen F, Meghvansi MK. Preliminary phytochemicals analysis of some important medicinal and aromatic plants. Advancesin Biological Research 2009; 3: 188-95.
28.	Kapoor LD, Singh A, Kapoor SL, Srivastava SN. Survey of Indian plants for saponins, alkaloids and flavonoids. I Lloydia 1969; 32: 297-304.
29.	Van-Burden TP, Robinson WC. Formation of complexes between protein and tannin acid. J Agri Food Chem 1981; 1: 77-82.
30.	Obadoni BO, Ochuko PO. Phytochemical studies and comparative efficacy of the crude extract of some homeostatic plants in Edo and Delta states of Nigeria. Global J Pure Appl Sci 2001; 8: 203-8.
31.	Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdicphosphotungstic acid reagents. Am J Enol Vitic 1965; 16: 144-58. DOI URL
32.	Sakanaka S, Tachibana Y, Okada Y. Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinohacha). Food Chem 2005; 89: 569-75. DOI URL
33.	McDonald S, Prenzler PD, Antolovich M, Robards K. Phenolic content and antioxidant activity of olive extracts. Food Chem 2001; 73: 73-84. DOI URL
34.	Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002; 10: 178-82.
35.	Oyaizu M. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 1986; 44: 307-15. DOI URL
36.	Velavan S, Arivoli P, Mahadevan K. Biological reduction of silver nanoparticles using Cassia auriculata flower extract and evaluation of their in vitro antioxidant activities. J Nanosci Nanotechnol 2012; 2: 30-5.
37.	Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Food Sci Technol-LWT 1995; 28: 25-30. DOI URL
38.	Pick E, Mizel D. Rapid microassays for the measurement of superoxide and hydrogen peroxide production by macrophages in culture using an automatic enzyme immunoassay reader. J Immunol Methods 1981; 46: 211-26. DOI PMID
39.	Mathew S, Abraham TE. In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies. Food Chem Toxicol 2006; 44: 198-206. DOI URL
40.	Kwon Y, Apostolidis E, Shetty K. Inhibitory potential of wine and tea against α-amylase and α-glucosidase for management of hyperglycemia linked to type 2 diabetes. J Food Biochem 2006; 2: 15-31.
41.	Ali H, Houghton PJ, Soumyanath A. alpha-amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. J Ethnopharmacol 2006; 107: 449-55. DOI PMID
42.	Lineweaver H, Burk D. The determination of enzyme dissociation constants. J Am Chem Soc 1934; 56: 658-66. DOI URL
43.	Hofstee BH. On the evaluation of the constants Vm and KM in enzyme reactions. Science 1952; 116: 329-31. PMID
44.	Dowd JE, Riggs DS. A comparison of estimates of michaelis-menten kinetic constants from various linear transformations. J Biol Chem 1965; 240: 863-9. PMID
45.	Cornish-Bowden A, Cárdenas ML. Hexokinase and 'glucokinase' in liver metabolism. Trends Biochem Sci 1991; 16: 281-2. PMID
46.	Dixon M, Webb EC. Enzymes. London: Longmans, 1964: 22.
47.	Ahmed M, Batista J, Rocha T, Mazzanti CM, Hassan W, Morsch VM. Comparative study of the inhibitory effect of antidepressants on cholinesterase activity in Bungarus sindanus (krait) venom, human serum and rat striatum. J Enzyme Inhib Med Chem 2008; 23: 912-7. DOI URL
48.	Rocha JB, Emanuelli T, Pereira ME. Effects of early undernutrition on kinetic parameters of brain acetylcholinesterase from adult rats. Acta Neurobiol Exp (Wars) 1993; 53: 431-7.
49.	Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-54. PMID
50.	Steel RGD, Torrie JH. Principles and procedures of statistics:a biometrical approach. New York: McGraw-Hill, 1984: 88.
51.	Jaradat N, Hussen F, Ali AA. Preliminary phytochemical screening, quantitative estimation of total flavonoids, total phenols and antioxidant activity of ephedra alata decne. J Mater Environ Sci 2015; 6: 1771-8.
52.	Wolfe K, Wu X, Liu RH. Antioxidant activity of apple peels. J Agric Food Chem 2003; 51: 609-14. DOI URL
53.	Koleckar V, Kubikova K, Rehakova Z, et al. Condensed and hydrolysable tannins as antioxidants influencing the health. Mini Rev Med Chem 2008; 8: 436-47. DOI URL
54.	Bahari EA, Zaaba NE, Haron N, Dasiman R, Amom Z. Antioxidant activity characterization, phytochemical screening, and proximate analysis of Cermela Hutan (Phyllanthus gomphocarpus Hook. F) roots and leaves. Med Sci Monit Basic Res 2014; 20: 170-5. DOI URL
55.	Aliev G, Obrenovich ME, Reddy VP, et al. Antioxidant therapy in Alzheimer's disease: theory and practice. Mini Rev Med Chem 2008; 8: 1395-406. DOI URL
56.	Matsuda H, Morikawa T, Yoshikawa M. Antidiabetogenic constituents from several natural medicines. Pure Appl Chem 2002; 74: 1301-8. DOI URL
57.	Shai LJ, Masoko P, Mokgotho MP, et al. Yeast alpha glucosidase inhibitory and antioxidant activities of six medicinal plants collected in Phalaborwa, South Africa. S Afr J Bot 2010; 76: 465-70. DOI URL
58.	Matsui T, Ueda T, Oki T, Sugita K, Terahara N, Matsumoto K. alpha-Glucosidase inhibitory action of natural acylated anthocyanins. 1. Survey of natural pigments with potent inhibitory activity. J Agric Food Chem 2001; 49: 1948-51. DOI URL
59.	Marrelli M, Amodeo V, Statti G, Conforti F. Biological properties and bioactive components of allium cepa l.: focus on potential benefits in the treatment of obesity and related comorbidities. Molecules 2018; 24: 39. DOI URL
60.	Barrett ML, Udani JK. A proprietary alpha-amylase inhibitor from white bean (Phaseolus vulgaris): a review of clinical studies on weight loss and glycemic control. Nutr. J 2011; 10: 24. DOI PMID
61.	Schneider LS. Treatment of Alzheimer's disease with cholinesterase inhibitors. Clin Geriatr Med 2001; 17: 337-58. PMID
62.	Khan I, Nisar M, Khan N, et al. Structural insights to investigate Conypododiol as a dual cholinesterase inhibitor from Asparagus adscendens. Fitoterapia 2010; 81: 1020-5. DOI PMID
63.	Ovais M, Ayaz M, Khalil AT, et al. HPLC-DAD finger printing, antioxidant, cholinesterase, and α-glucosidase inhibitory potentials of a novel plant Olax nana. BMC Complement Altern Med 2018; 18: 1. DOI URL
64.	Steele M, Stuchbury G, Münch G. The molecular basis of the prevention of Alzheimer's disease through healthy nutrition. Exp Gerontol 2007; 42: 28-36. PMID
65.	Mecocci P, Polidori MC. Antioxidant clinical trials in mild cognitive impairment and Alzheimer's disease. Biochim Biophys Acta 2012; 1822: 631-8. DOI PMID

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