Phytochemical analysis and inhibitory effects of Calligonum polygonoides on pancreatic α-amylase and β-glucosidase enzymes

doi:10.19852/j.cnki.jtcm.2022.03.009

Abstract

Abstract:

OBJECTIVES: To estimate the existence of phyto-chemicals and then to determine the antidiabetic activity against α-amylase and β-glucosidase inhibition in vitro.

METHODS: The study was carried out by following standard procedures.

RESULTS: Phytochemicals analysis indicated the presence of different phytochemicals. The total phenolic content was 6.055 mg GAE/g and the total flavonoid content was 5.706 mg RU/g in the plant extract. The total saponins, alkaloids, and tannins contents were (0.044%), (2.88%) and (2.862 nm) respectively. α-amylase inhibition activity of Calligonum polygonoides (CP) extract was 70% with IC50 of 610 μg/mL and that of β-gluco-sidase inhibition activity was 65% with IC50 of 640 µg/mL.

CONCLUSION: The findings reported for the first time the antidiabetes-promoting effects of an extract of CP in vitro, thus validating their promising anti-diabetes potential.

Key words: Calligonum polygonoides, phytochemicals, alpha-amylases, beta-glucosidase

Mushtaq Ahmed, Naila Sher, Nadia Mushtaq, Rahmat Ali Khan. Phytochemical analysis and inhibitory effects of Calligonum polygonoides on pancreatic α-amylase and β-glucosidase enzymes[J]. Journal of Traditional Chinese Medicine, 2022, 42(3): 426-431.

Figures/Tables 6

Figure 1 Standard calibration curve of Rutin

Figure 2 Standard calibration curve of Gallic acid

Figure 3 IC50 was calculated for Calligonum polygonoides and tagipmet from the simple plot of % activity vs % inhibition.

Figure 4 In-vitro antidiabetic activities by α-amylase method

Figure 5 IC50 was calculated for Calligonum polygonoides and tagipmet from the simple plot of % activity vs % inhibition.

Figure 6 In-vitro antidiabetic activities by β-glucosidase method

References 44

1	Farnsworth NR. The role of ethnopharmacology in drug development. Ciba Found Symp 1990; 154:2-11. PMID
2	Anandarajagopal K, Sudhahar D, Ajaykumar TV, et al. Evaluation of CNS depressant activity of aerial parts of Basella alba Linn. J Pharmacol Toxicol 2011; 1.
3	Luo Q, Cai Y, Yan J, et al. Hypoglycemic and hypolipidemic effects and antioxidant activity of fruit extracts from Lycium barbarum. Life Sci 2004; 76:137-49. DOI URL
4	Risérus U, Willett WC, Hu FB. Dietary fats and prevention of type 2 diabetes. Prog. Lipid Res 2009; 48:44-51. DOI PMID
5	Gupta R, Gigras P, Mohapatra H, et al. Microbial α-amylases: a biotechnological perspective. Process Biochem 2003; 38:1599-616. DOI URL
6	Anam K, Widharna RM, Kusrini D. α-Glucosidase inhibitor of Terminalia species. Int J Pharmacol 2009; 5:277-80. DOI URL
7	Fred-Jaiyesimi A, Kio A, Richard W. α-Amylase inhibitory effect of 3β-olean-12-en-3-yl (9Z)- hexadec-9-enoate isolated from Spondias mombin leaf. Food Chem 2009; 116:285-8. DOI URL
8	Ghadyale V, Takalikar S, Haldavnekar V, et al. Effective control of postprandial glucose level through inhibition of intestinal alpha glucosidase by Cymbopogon martinii (Roxb.). J Evid Based Complementary Altern Med 2012; 2012:372909.
9	Singh SK, Rai PK, Jaiswal D, et al. Evidence-based Critical Evaluation of Glycemic Potential of Cynodon dactylon. J Evid Based Complementary Altern Med 2008; 5:415-20.
10	Katewa SS, Galav PK. Traditional herbal medicines from Shekhawati region of Rajasthan. Indian J Tradit Knowl 2005; 4:237-45.
11	Lichterman BL. Aspirin: the story of a wonder drug. Bri Med J 2004; 329:1408. DOI URL
12	Sofowora A. Medicinal plants and traditional medicine in Africa. Ibadan [[u.a.]: Spectrum Books Ltd.; 1993; 191-289.
13	Harborne JB. Phytochemical methods: a guide to modern techniques of plant analysis. London: Chapman and Hall; 1973; 1-36.
14	Trease GE, Evans MD. A Textbook of Pharmacognosy. 13th ed. Builler Tindall and Caussel London 1989; 44.
15	Siddiqui S, Verma A, Rather AA, et al. Preliminary phytochemicals analysis of some important medicinal and aromatic plants. Adv Bio Res 2009; 3:188-95.
16	Van-Burden TP, Robinson WC. Formation of complexes between protein and tannin acid. J Agri Food Chem 1981; 1:77-82.
17	Obadoni BO, Ochuko PO. Phytochemical studies and comparative efficacy of the crude extract of some homeostatic plants in Edo and Delta states of Nigeria. Global J Pure Appl Sci 2001; 8:203-8.
18	Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdicphosphotungstic acid reagents. Am J Enol Vitic 1965; 16:144-58.
19	Sakanaka S, Tachibana Y, Okada Y. Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinohacha). Food Chem 2005; 89:569-75. DOI URL
20	Malik CP, Singh MB. Plant Enzymology and Histoenzymology. Kalyani publishers, New Delhi 1981: 278.
21	Gupta R, Misra A. In vitro antidiabetic activity of pentacyclic tritrprnoida and fatty acid ester from bauhinia purpurea. Br J Diabetes Vasc 2007; 7:16-25.
22	Krishnaveni S, Balasubramanian T, Sadasivam S. Sugar distribution in sweet stalk sorghum. Food Chem 1984; 15:229-32. DOI URL
23	Hasani-Ranjbar S, Larijani B, Abdollah M. A systematic review of Iranian medicinal plants useful in diabetes mellitus. Arch Med Sci 2008: 285-92.
24	Tsakala O, Pen RS, Miemanang K, et al. Paullinoside A and Paullinomide a: a new cerebroside and a new ceramide from the leaves of Paullinia pinnata. Z Naturfosch 2006; 61:1123-7.
25	Okwu DE, Ekeke O. Phytochemical screening and mineral composition of chewingsticks in South Eastern Nigeria. Int J Pure Appl Sci 2003; 9:235-8.
26	Pietta PG. Flavonoids as antioxidants. J Nat Prod 2000; 63:1035-42. PMID
27	Shehzad A, Khan S, Sup LY. Future oncology. Precis Future Med 82012: 179-90.
28	Elekofehinti OO, Kamdem JP, Kade IJ, et al. Saponins fromSolanum anguivi lam. fruit exhibit in vitro and in vivo antioxidant activities in alloxan-induced oxidative stress. Asian J Pharm Clin Res 2012; 6:249-54.
29	Betteridge DJ. What is oxidative stress. Metabolism 2000; 49:3-8.
30	Velioglu YS, Mazza G, Gao L, et al. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 1998; 46 :4113-7. DOI URL
31	Thielecke F, Boschmann M. The potential role of green tea catechins in the prevention of the metabolic syndrome - a review. Phytochemistry 2009; 70:11-24. DOI PMID
32	Lim SS, Jung SH, Ji J, et al. Synthesis of flavonoids and their effects on aldose reductase and sorbitol accumulation in streptozotocininduced diabetic rat tissues. Pharm Pharmacol 2001; 53:653-68. DOI URL
33	Venkatesh S, Reddy GD, Reddy BM, et al. Antihyperglycemic activity of Caralluma attenuata. Fitoterapia 2003; 74:274-9. PMID
34	Suba V, Murugesan T, Arunachalam G, et al. Anti-diabetic potential of Barleria lupulina extract in rats. Int. J. Phytomedicine 2004; 11:202-5.
35	Barrett A, Ndou T, Hughey CA, et al. Inhibition of alpha-amylase and glucoamylase by tannins extracted from cocoa, pomegranates, cranberries, and grapes. J Agric Food Chem 2013; 61:1477-86. DOI URL
36	Jadhav R, Puchchakayala G. Hypoglycemic and antidiabetic activity of flavonoids: boswellic acid, ellagic acid, quercetin, rutin on streptozotocinnicotinamide induced type 2 diabetic rats. Int J Pharm Pharm Sci 2012; 1:251-6.
37	Babu PV, Liu D, Gilbert ER. Recent advances in understanding the anti-diabetic actions of dietary flavonoids. J Nutr Biochem 2013; 24:1777-89. DOI URL
38	Lin Y, Shi R, Wang X, et al. Luteolin, a flavonoid with potential for cancer prevention and therapy. Curr Cancer Drug Targets 2008; 8:634-46. DOI URL
39	Bannour M, Fellah B, Rocchetti G, et al. Phenolic profiling and antioxidant capacity of Calligonum azel Maire, a Tunisian desert plant. Food Res Int 2017; 101:148-54. DOI PMID
40	Tadera K, Minami Y, Takamatsu K, et al. Inhibition of alpha-glucosidase and alpha-amylase by flavonoids. J Nutr Sci Vitaminol (Tokyo) 2006; 52:149-53. DOI URL
41	Dendup T, Prachyawarakorn V, Pansanit A, et al. Alpha-Glucosidase inhibitory activities of isoflavanones, isoflavones, and pterocarpans from Mucuna pruriens. Planta Med 2014; 80:604-8. DOI URL
42	Lopez SN, Sierra MG, Gattuso SJ, et al. An unusual homoisoflavanone and a structurally-related dihydrochalcone from Polygonum ferrugineum (Polygonaceae). Phytochemistry 2006; 67:2152-8. DOI URL
43	Tundis R, Loizzo MR, Menichini F. Natural products as alpha-amylase and alpha-glucosidase inhibitors and their hypoglycaemic potential in the treatment of diabetes: an update. Mini Rev Med Chem 2010; 10:315-31. PMID
44	Sales PM, Souza PM, Simeoni LA, et al. α-Amylase inhibitors: a review of raw material and isolated compounds from plant source. J Pharm Pharm Sci: a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques 2012; 15:141-83.

[1]	Qudsia Tabassam, Tahir Mehmood, Sibtain Ahmed, Farooq Anwar, Abdul Rauf Raza. Characterization and anticancer potential of Withania somnifera fruit bioactives (a native species to Pakistan) using gas chromatography-mass spectrometer, nuclear magnetic resonance and liquid chromatography-mass spectrometry-electrospray ionization [J]. Journal of Traditional Chinese Medicine, 2022, 42(6): 908-918.
[2]	?nder Aybast?er;. Efficacy of methanol-water extract of Inula helenium root against oxidative DNA damage [J]. Journal of Traditional Chinese Medicine, 2021, 41(2): 293-300.
[3]	Xue Mei, Bian Yong, Liu Yalin, Zhou Junjie, Xu Jianjun, Zhang Liang, Huang Xi. Danggui Buxue decoction ameliorates lipid metabolic defects involved in the initiation of diabetic atherosclerosis; identification of active compounds [J]. Journal of Traditional Chinese Medicine, 2020, 40(3): 414-421.
[4]	Hu Xuan, Wang Dan, Pang Yuxin, Wu Zhisheng, Huan Hengfu, Chen Zhijian, Li Weidong. Development of chromatographic fingerprint for quality analysis of diploid and tetraploid Lonicera japonica [J]. Journal of Traditional Chinese Medicine, 2020, 40(1): 73-82.
[5]	Ghosia Lutfullah, Asma Shah, Kafeel Ahmad, Jamila Haider. Phytochemical screening, antioxidant and antibacterial properties of daphne mucronata [J]. Journal of Traditional Chinese Medicine, 2019, 39(06): 764-771.
[6]	Pouran Andarkhor, Mahmood Khodadoost, Mohammad Kamalinejad, Ramin Talaie, Latif Gachkar, Arman Zargaran. Twelve vegetables used for prevention and treatment of hemorrhoids in Persian Medicine [J]. Journal of Traditional Chinese Medicine, 2019, 39(04): 466-473.
[7]	Samkeliso Takaidza, Fanyana Mtunzi, Michael Pillay. Analysis of the phytochemical contents and antioxidant activities of crude extracts from Tulbaghia species [J]. Journal of Traditional Chinese Medicine, 2018, 38(02): 272-279.
[8]	Mohammad Hosein Farzaei, Roodabeh Bahramsoltani, Ali Ghobadi, Fatemeh Farzaei, Fariba Najafi. Pharmacological activity of Mentha longifolia and its phytoconstituents [J]. Journal of Traditional Chinese Medicine, 2017, 37(05): 710-720.
[9]	Akuodor Godwin Christian, Ezeonu Chinonyelum Thecla, Essien Augustine Dick, Asika Ebere Chile, Chilaka Kingsley Chimsorom, Nwachukwu Daniel Ckukwu, Nwobodo Ndubuisi Nwobodo. In vivo antiplasmodial activity of Bombax buonopozense root bark aqueous extract in mice infected by Plasmodium berghei [J]. Journal of Traditional Chinese Medicine, 2017, 37(04): 431-435.
[10]	Assie Jokar, Fatemeh Masoomi, Omid Sadeghpour, Mohsen Nassiri-Toosi, Shokouhsadat Hamedi. Potential therapeutic applications for Terminalia chebula in Iranian traditional medicine [J]. Journal of Traditional Chinese Medicine, 2016, 36(02): 250-254.

Home

Online First

Author Center

Reviewer Center

Issues

Announcement

About Us