Journal of Traditional Chinese Medicine ›› 2023, Vol. 43 ›› Issue (3): 514-522.DOI: 10.19852/j.cnki.jtcm.20230328.001
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Waggle needling Yanglingquan (GB34) enhances cerebral blood flow and ameliorates condition of spastic paresis in rats with middle cerebral artery occlusion induced by surgical nylon suture
ZHANG Zhou1, MU Jiedan1, MA Liangxiao1,2(
), YU Wenyan1, SUN Tianyi1, QIAN Xu1, TIAN Yuan1, ZHANG Yidan1, WANG Junxiang3
- 1 School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China
2 the Key Unit of State Administration of Traditional Chines Medicine, Evaluation of Characteristic Acupuncture Therapy, Beijing 100029, China
3 School of Nursing, Beijing University of Chinese Medicine, Beijing 100029, China
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Received:2022-01-12Accepted:2022-04-10Online:2023-06-15Published:2023-03-28 -
Contact:Prof. MA Liangxiao, School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China and the Key Unit of State Administration of Traditional Chines Medicine, Evaluation of Characteristic Acupuncture Therapy, Beijing 100029, China. maliangxiao@vip.sina.com. Telephone: +86-10-53912199 -
Supported by:Study on the Mechanism of Waggle Needling GB34 Relieving Spasticity in Post-stroke Rats Based on KCC2-GABAA Receptor Pathway(81774417);Research and Development of Physical Therapy Technology and Equipment for Regulation of Human Functional State(2020-JYB-ZDGG-062)
Cite this article
ZHANG Zhou, MU Jiedan, MA Liangxiao, YU Wenyan, SUN Tianyi, QIAN Xu, TIAN Yuan, ZHANG Yidan, WANG Junxiang. Waggle needling Yanglingquan (GB34) enhances cerebral blood flow and ameliorates condition of spastic paresis in rats with middle cerebral artery occlusion induced by surgical nylon suture[J]. Journal of Traditional Chinese Medicine, 2023, 43(3): 514-522.
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Figure 1 Experimental design of this study and the effect of acupuncture at Yanglingquan (GB34) on neurological deficit and muscle tone in MCAO-induced SP rats A: experimental design of this study. Behavioral tests included the modified neurological severity score and modified Ashworth scale; B: effect of acupuncture at Yanglingquan (GB34) on neurological deficit in MCAO-induced spastic paresis rats. The change of neurological deficit assessed by mNSS at days 0, 1, 3, 5, 7 and 9; C: effect of acupuncture at Yanglingquan (GB34) on muscle tone in MCAO-induced spastic paresis rats. The change of hyper muscle tone assessed by MAS at days 0, 1, 3, 5, 7 and 9. Control group: Rats without any intervention (n = 9). Sham group: Rats received sham operation (n = 9); Model group: MCAO rats without treatment (n = 10); Waggle needling group: MCAO rats + waggle needling Yanglingquan (GB34) for 6 d (n = 9); Perpendicular needling group: MCAO rats + perpendicular needling Yanglingquan (GB34) for 6 d (n = 9). MCAO: middle cerebral artery occlusion; GB34: Yanglingquan acupoint; RT-qPCR: real‐time quantitative polymerase chain reaction; mNSS: the modified neurological severity score; MAS: Modified Ashworth Scale; SP: spastic paresis; Control: control group; Sham: sham group; Model: model group; WN: waggle needling group; PN: perpendicular needling group. All data were expressed as mean ± standard error of mean. aP<0.01, bP<0.001, eP<0.01, versus model group; cP<0.01, dP<0.001 versus PN group.
Figure 1 Experimental design of this study and the effect of acupuncture at Yanglingquan (GB34) on neurological deficit and muscle tone in MCAO-induced SP rats A: experimental design of this study. Behavioral tests included the modified neurological severity score and modified Ashworth scale; B: effect of acupuncture at Yanglingquan (GB34) on neurological deficit in MCAO-induced spastic paresis rats. The change of neurological deficit assessed by mNSS at days 0, 1, 3, 5, 7 and 9; C: effect of acupuncture at Yanglingquan (GB34) on muscle tone in MCAO-induced spastic paresis rats. The change of hyper muscle tone assessed by MAS at days 0, 1, 3, 5, 7 and 9. Control group: Rats without any intervention (n = 9). Sham group: Rats received sham operation (n = 9); Model group: MCAO rats without treatment (n = 10); Waggle needling group: MCAO rats + waggle needling Yanglingquan (GB34) for 6 d (n = 9); Perpendicular needling group: MCAO rats + perpendicular needling Yanglingquan (GB34) for 6 d (n = 9). MCAO: middle cerebral artery occlusion; GB34: Yanglingquan acupoint; RT-qPCR: real‐time quantitative polymerase chain reaction; mNSS: the modified neurological severity score; MAS: Modified Ashworth Scale; SP: spastic paresis; Control: control group; Sham: sham group; Model: model group; WN: waggle needling group; PN: perpendicular needling group. All data were expressed as mean ± standard error of mean. aP<0.01, bP<0.001, eP<0.01, versus model group; cP<0.01, dP<0.001 versus PN group.
Figure 2 Effect of acupuncture at Yanglingquan (GB34) on cerebral blood flow recovery in MCAO-induced SP rats A: representative LSI images assessing the CBF. The dynamic change of CBF was recorded in the ROI; B: summary data of cortical CBF in ROI at 5 min before MCAO, 5 min after MCAO and after 6 days’ treatment. Intervention included waggle needling and perpendicular needling Yanglingquan (GB34) in each group. C: representative LSI images of cortical CBF in different groups at 5 min before MCAO, 5 min after MCAO and after 6 days’ treatment. c1-c5: The representative LSI images of rats in Control group, Sham group, Model group, Waggle needling group and Perpendicular needling group at 5 min before MCAO. c6-c10: The representative LSI images of rats in Control group, Sham group, Model group, Waggle needling group and Perpendicular needling group at 5 min after MCAO. c11-c15: The representative LSI images of rats in Control group, Sham group, Model group, Waggle needling group and Perpendicular needling group at 5 min after 6 days’ treatment. Control group: Rats without any intervention (n = 9). Sham group: Rats received sham operation (n = 9). Model group: MCAO rats without treatment (n = 10). Waggle needling group: MCAO rats + waggle needling Yanglingquan (GB34) for 6 d (n = 9). Perpendicular needling group: MCAO rats + perpendicular needling Yanglingquan (GB34) for 6 d (n = 9). ROI: region of interest. MCAO: middle cerebral artery occlusion; SP: spastic paresis; LSI: laser speckle imaging; CBF: cortical cerebral blood flow; ROI: region of interest; Pre MCAO: 5 min pre MCAO operation; Post MCAO: 5 min post MCAO operation; After Intervention: after intervention at day 9. Control: control group; Sham: sham group; Model: model group; WN: waggle needling group; PN: perpendicular needling group. Scale bar = 3 mm. All data were expressed as mean ± standard error of mean. aP<0.001 versus model group; bP<0.001 versus PN group.
Figure 2 Effect of acupuncture at Yanglingquan (GB34) on cerebral blood flow recovery in MCAO-induced SP rats A: representative LSI images assessing the CBF. The dynamic change of CBF was recorded in the ROI; B: summary data of cortical CBF in ROI at 5 min before MCAO, 5 min after MCAO and after 6 days’ treatment. Intervention included waggle needling and perpendicular needling Yanglingquan (GB34) in each group. C: representative LSI images of cortical CBF in different groups at 5 min before MCAO, 5 min after MCAO and after 6 days’ treatment. c1-c5: The representative LSI images of rats in Control group, Sham group, Model group, Waggle needling group and Perpendicular needling group at 5 min before MCAO. c6-c10: The representative LSI images of rats in Control group, Sham group, Model group, Waggle needling group and Perpendicular needling group at 5 min after MCAO. c11-c15: The representative LSI images of rats in Control group, Sham group, Model group, Waggle needling group and Perpendicular needling group at 5 min after 6 days’ treatment. Control group: Rats without any intervention (n = 9). Sham group: Rats received sham operation (n = 9). Model group: MCAO rats without treatment (n = 10). Waggle needling group: MCAO rats + waggle needling Yanglingquan (GB34) for 6 d (n = 9). Perpendicular needling group: MCAO rats + perpendicular needling Yanglingquan (GB34) for 6 d (n = 9). ROI: region of interest. MCAO: middle cerebral artery occlusion; SP: spastic paresis; LSI: laser speckle imaging; CBF: cortical cerebral blood flow; ROI: region of interest; Pre MCAO: 5 min pre MCAO operation; Post MCAO: 5 min post MCAO operation; After Intervention: after intervention at day 9. Control: control group; Sham: sham group; Model: model group; WN: waggle needling group; PN: perpendicular needling group. Scale bar = 3 mm. All data were expressed as mean ± standard error of mean. aP<0.001 versus model group; bP<0.001 versus PN group.
Figure 3 Effect of acupuncture at Yanglingquan (GB34) on the expressions of GABAAγ2 and KCC2 in the ischemic cortex in SP rats A: representative Western blots of GABAAγ2 and KCC2 in the cortex; B, C: protein levels of GABAAγ2 and KCC2 in the cortex; D, E: mRNA levels of GABAAγ2 and KCC2 in the cortex. Control group: rats without any intervention (n = 9). Sham group: rats received sham operation (n = 9). Model group: MCAO rats without treatment (n = 9). Waggle needling group: MCAO rats + waggle needling Yanglingquan (GB34) for 6 d (n = 9). Perpendicular needling group: MCAO rats + perpendicular needling Yanglingquan (GB34) for 6 d (n = 9). GABAAγ2: γ2 subunit of the γ-aminobutyric acid receptor A; KCC2: K+-C-cotransporter 2; SP: spastic paresis; Control: control group; Sham: sham group; Model: model group; WN: waggle needling group; PN: perpendicular needling group. All data were expressed as mean ± standard error of mean. aP<0.001 versus Model group; bP<0.01, cP<0.05 versus PN group.
Figure 3 Effect of acupuncture at Yanglingquan (GB34) on the expressions of GABAAγ2 and KCC2 in the ischemic cortex in SP rats A: representative Western blots of GABAAγ2 and KCC2 in the cortex; B, C: protein levels of GABAAγ2 and KCC2 in the cortex; D, E: mRNA levels of GABAAγ2 and KCC2 in the cortex. Control group: rats without any intervention (n = 9). Sham group: rats received sham operation (n = 9). Model group: MCAO rats without treatment (n = 9). Waggle needling group: MCAO rats + waggle needling Yanglingquan (GB34) for 6 d (n = 9). Perpendicular needling group: MCAO rats + perpendicular needling Yanglingquan (GB34) for 6 d (n = 9). GABAAγ2: γ2 subunit of the γ-aminobutyric acid receptor A; KCC2: K+-C-cotransporter 2; SP: spastic paresis; Control: control group; Sham: sham group; Model: model group; WN: waggle needling group; PN: perpendicular needling group. All data were expressed as mean ± standard error of mean. aP<0.001 versus Model group; bP<0.01, cP<0.05 versus PN group.
Figure 4 Effect of acupuncture at Yanglingquan (GB34) on the expressions of GABAAγ2 and KCC2 in the lumbar enlargement in SP rats A: representative Western blots of GABAAγ2 and KCC2 in the lumbar enlargement; B, C: protein levels of GABAAγ2 and KCC2 in the lumbar enlargement; D, E: mRNA levels of GABAAγ2 and KCC2 in the lumbar enlargement. Control group: Rats without any intervention (n = 9). Sham group: Rats received sham operation (n = 9). Model group: MCAO rats without treatment (n = 9). Waggle needling group: MCAO rats + waggle needling Yanglingquan (GB34) for 6 d (n = 9). Perpendicular needling group: MCAO rats + perpendicular needling Yanglingquan (GB34) for 6 d (n = 9). GABAAγ2: γ2 subunit of the γ-aminobutyric acid receptor A; KCC2: K+-Cl-cotransporter 2; SP: spastic paresis; Control: Control group; Sham: Sham group; Model: Model group; WN: Waggle needling group; PN: perpendicular needling group. All data were expressed as mean ± standard error of mean. aP<0.001, dP<0.01 versus Model group; bP<0.001, cP<0.01 versus PN group.
Figure 4 Effect of acupuncture at Yanglingquan (GB34) on the expressions of GABAAγ2 and KCC2 in the lumbar enlargement in SP rats A: representative Western blots of GABAAγ2 and KCC2 in the lumbar enlargement; B, C: protein levels of GABAAγ2 and KCC2 in the lumbar enlargement; D, E: mRNA levels of GABAAγ2 and KCC2 in the lumbar enlargement. Control group: Rats without any intervention (n = 9). Sham group: Rats received sham operation (n = 9). Model group: MCAO rats without treatment (n = 9). Waggle needling group: MCAO rats + waggle needling Yanglingquan (GB34) for 6 d (n = 9). Perpendicular needling group: MCAO rats + perpendicular needling Yanglingquan (GB34) for 6 d (n = 9). GABAAγ2: γ2 subunit of the γ-aminobutyric acid receptor A; KCC2: K+-Cl-cotransporter 2; SP: spastic paresis; Control: Control group; Sham: Sham group; Model: Model group; WN: Waggle needling group; PN: perpendicular needling group. All data were expressed as mean ± standard error of mean. aP<0.001, dP<0.01 versus Model group; bP<0.001, cP<0.01 versus PN group.
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