Journal of Traditional Chinese Medicine ›› 2023, Vol. 43 ›› Issue (3): 523-532.DOI: 10.19852/j.cnki.jtcm.20230404.005
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YE Yujuan, WEI Yuting, JIA Jing, YAN Xingke()
Received:
2022-02-22
Accepted:
2022-06-16
Online:
2023-06-15
Published:
2023-04-04
Contact:
YAN Xingke, College of Acupuncture and Massage, Gansu University of Traditional Chinese medicine, Lanzhou 730000, China. yanxingke@126.com. Telephone: +86-931-5161180
Supported by:
YE Yujuan, WEI Yuting, JIA Jing, YAN Xingke. Efficacy of needling Baihui (GV20), Neiguan (PC6), Shenmen (HT7) and Taichong (LR3) on cerebral cortical blood oxygen level in rats with insomnia[J]. Journal of Traditional Chinese Medicine, 2023, 43(3): 523-532.
Group | n | SL | ST |
---|---|---|---|
Blank | 10 | 2.7±0.7 | 102.2±5.2 |
Model | 10 | 4.4±0.7a | 78.1±14.2a |
Grabbing | 10 | 4.5±0.8 | 74.7±2.4 |
Western Medicine | 10 | 2.9±0.6b | 94.8±13.6b |
Acupuncture | 10 | 3.0±0.5b | 88.1±3.9b |
Sham acupuncture | 10 | 4.4±1.0c | 69.3±4.5c |
Table 1 Comparison of pentobarbital sodium sleep test results of rats in each group after treatment ($\bar{x}±s$, t/min)
Group | n | SL | ST |
---|---|---|---|
Blank | 10 | 2.7±0.7 | 102.2±5.2 |
Model | 10 | 4.4±0.7a | 78.1±14.2a |
Grabbing | 10 | 4.5±0.8 | 74.7±2.4 |
Western Medicine | 10 | 2.9±0.6b | 94.8±13.6b |
Acupuncture | 10 | 3.0±0.5b | 88.1±3.9b |
Sham acupuncture | 10 | 4.4±1.0c | 69.3±4.5c |
Group | n | OE% | OT% |
---|---|---|---|
Blank | 10 | 65.9±5.4 | 66.5±4.3 |
Model | 10 | 40.0±5.3a | 46.8±3.9a |
Grabbing | 10 | 42.1±5.3 | 50.0±2.7 |
Western Medicine | 10 | 57.8±3.9b | 60.7±6.1b |
Acupuncture | 10 | 64.3±2.3bc | 68.2±6.0bc |
Sham acupuncture | 10 | 43.7±4.2d | 43.1±5.7d |
Table 2 Comparison of elevated cross maze results of rats in each group after treatment ($\bar{x}±s$)
Group | n | OE% | OT% |
---|---|---|---|
Blank | 10 | 65.9±5.4 | 66.5±4.3 |
Model | 10 | 40.0±5.3a | 46.8±3.9a |
Grabbing | 10 | 42.1±5.3 | 50.0±2.7 |
Western Medicine | 10 | 57.8±3.9b | 60.7±6.1b |
Acupuncture | 10 | 64.3±2.3bc | 68.2±6.0bc |
Sham acupuncture | 10 | 43.7±4.2d | 43.1±5.7d |
Group | n | Vertical score | Horizontal crossing times | Center grid score | Modification times |
---|---|---|---|---|---|
Blank | 10 | 11.2±1.5 | 26.7±4.1 | 19.6±1.7 | 1.7±0.8 |
Model | 10 | 5.9±1.9a | 16.8±2.2a | 14.3±1.2a | 3.6±1.1a |
Grabbing | 10 | 6.4±1.7 | 17.1±2.2 | 13.7±1.6 | 3.3±1.1 |
Western Medicine | 10 | 10.9±1.4b | 24.9±1.7b | 65.7±5.5b | 1.6±1.1b |
Acupuncture | 10 | 11.1±1.4b | 24.8±2.7b | 19.3±2.4b | 1.8±1.0c |
Sham acupuncture | 10 | 5.5±1.3d | 16.9±2.0d | 13.4±1.5d | 3.6±1.1d |
Table 3 Comparison of open field test results of rats in each group after treatment ($\bar{x}±s$)
Group | n | Vertical score | Horizontal crossing times | Center grid score | Modification times |
---|---|---|---|---|---|
Blank | 10 | 11.2±1.5 | 26.7±4.1 | 19.6±1.7 | 1.7±0.8 |
Model | 10 | 5.9±1.9a | 16.8±2.2a | 14.3±1.2a | 3.6±1.1a |
Grabbing | 10 | 6.4±1.7 | 17.1±2.2 | 13.7±1.6 | 3.3±1.1 |
Western Medicine | 10 | 10.9±1.4b | 24.9±1.7b | 65.7±5.5b | 1.6±1.1b |
Acupuncture | 10 | 11.1±1.4b | 24.8±2.7b | 19.3±2.4b | 1.8±1.0c |
Sham acupuncture | 10 | 5.5±1.3d | 16.9±2.0d | 13.4±1.5d | 3.6±1.1d |
Figure 1 Localization of brain area The blue area indicates that the cerebral cortex is inhibited, and the red area indicates that the cerebral cortex is activated. A: blood flow state of the prefrontal lobe of rats in the blank group, B: blood flow state of the prefrontal lobe of rats in the model group, C: blood flow state of the occipital lobe of rats in the blank group, D: blood flow state of the occipital lobe of rats in the model group.
Figure 2 Schematic diagram of cerebral cortical blood flow in resting state of rats in each group The closer to dark red, the higher the concentration of oxy-Hb, indicating that the activity of cerebral cortex is relatively active; The closer to dark blue, the lower the concentration of oxy-Hb, suggesting that the activity of cerebral cortex is inhibited to a certain extent. A: blank group; B: model group; C: grasping group; D: western medicine group; E: acupuncture group; F: sham acupuncture group. Blank group: rats without insomnia modeling with liver-Qi stagnation and acupuncture treatment; model group: rats with insomnia modeling with liver-Qi stagnation; grabbing group: rats with insomnia modeling with liver-Qi stagnation and grasping stimulation; Western Medicine group: rats with insomnia modeling with liver-Qi stagnation and eszopiclone treatment; acupuncture group: rats with insomnia modeling with liver-Qi stagnation and acupuncture treatment; sham acupuncture group: rats with insomnia modeling with liver-Qi stagnation and sham acupuncture treatment.
Figure 3 Determination of brain area The blue area indicates that the cerebral cortex is in an inhibited state, and the red area indicates that the cerebral cortex is in an activated state. A: blood flow state of the occipital lobe of rats in the blank group, B: blood flow state of the occipital lobe of rats in the model group. Blank group: rats without insomnia modeling with liver-Qi stagnation and acupuncture treatment; model group: rats with insomnia modeling with liver-Qi stagnation; grabbing group: rats with insomnia modeling with liver-Qi stagnation and grasping stimulation; Western Medicine group: rats with insomnia modeling with liver-Qi stagnation and eszopiclone treatment; acupuncture group: rats with insomnia modeling with liver-Qi stagnation and acupuncture treatment; sham acupuncture group: rats with insomnia modeling with liver-Qi stagnation and sham acupuncture treatment.
Figure 4 schematic diagram of cerebral cortical blood flow in resting state of rats in each group The closer to dark red, the higher the concentration of oxy Hb, indicating that the activity of cerebral cortex is relatively active; the closer to dark blue, the lower the concentration of oxy Hb, suggesting that the activity of cerebral cortex is inhibited to a certain extent. A: blank group; B: model group; C: grasping group; D: Western Medicine group; E: acupuncture group; F: sham acupuncture group. blank group: rats without insomnia modeling with liver-Qi stagnation and acupuncture treatment; model group: rats with insomnia modeling with liver-Qi stagnation; grabbing group: rats with insomnia modeling with liver-Qi stagnation and grasping stimulation; Western Medicine group: rats with insomnia modeling with liver-Qi stagnation and eszopiclone treatment; acupuncture group: rats with insomnia modeling with liver-Qi stagnation and acupuncture treatment; sham acupuncture group: rats with insomnia modeling with liver-Qi stagnation and sham acupuncture treatment.
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& China Academy of Chinese Medical Sciences
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