Journal of Traditional Chinese Medicine ›› 2022, Vol. 42 ›› Issue (5): 788-794.DOI: 10.19852/j.cnki.jtcm.20220815.003
• Research Articles • Previous Articles Next Articles
ZHOU Kaixuan, ZHANG Dong, BAO Huiwei, LI Lijing()
Received:
2021-07-16
Accepted:
2021-10-26
Online:
2022-08-15
Published:
2022-08-15
Contact:
LI Lijing
About author:
LI Lijing, College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China. lilijing66@163.com Telephone: +86-16689574396Supported by:
ZHOU Kaixuan, ZHANG Dong, BAO Huiwei, LI Lijing. Network pharmacology and molecular docking study on the effect of Kaempferol in treatment of metabolic associated fatty liver disease[J]. Journal of Traditional Chinese Medicine, 2022, 42(5): 788-794.
Figure 1 “compound-target-disease” network of kaempferol in the treatment of MAFLD MAFLD: metabolic associated fatty liver disease. The green represents the compound. The blue represents the common targets. The red represents the disease.
Gene | Degree | Betweenness Centrality | Closeness Centrality |
---|---|---|---|
AKT1 | 19 | 0.2748 | 0.6739 |
PTGS2 | 18 | 0.1162 | 0.6200 |
MMP9 | 16 | 0.1229 | 0.5962 |
EGFR | 16 | 0.0950 | 0.5962 |
Table 1 Core targets of kaempferol in the treatment of MAFLD
Gene | Degree | Betweenness Centrality | Closeness Centrality |
---|---|---|---|
AKT1 | 19 | 0.2748 | 0.6739 |
PTGS2 | 18 | 0.1162 | 0.6200 |
MMP9 | 16 | 0.1229 | 0.5962 |
EGFR | 16 | 0.0950 | 0.5962 |
Figure 2 Cytotoxicity results of kaempferol and oleic acid Con: control group; A: cytotoxicity of kaempferol, 10, 20, 40, 80, and 160 μM was the concentration gradient of kaempferol; B: cytotoxicity of oleic acid, 50, 100, 200, 400, and 800 μM was the concentration gradient of oleic acid. The data are expressed as the mean ± standard deviation (n = 6). aP < 0.05 versus the control group.
Figure 3 Lipid deposition was observed by oil red staining (×400) A: control group; B: model group, treated with 100 μM oleic acid; C: low-dose kaempferol treatment group, treated with 100 μM oleic acid and 10 μM kaempferol; D: high-dose kaempferol treatment group: treated with 100 μM oleic acid and 20 μM kaempferol.
Figure 4 Effect of kaempferol on PI3K, AKT, Beclin-1, NF-κB and Caspase-3 protein expression A: Western bloting bands; B, C: histogram of Western blotting results. Con: control group; Mod: treated with 100 μM oleic acid; KL: treated with 100 μM oleic acid and 10 μM kaempferol; KH: treated with 100 μM oleic acid and 20 μM kaempferol. PI3K: phosphatidylinositol-3-kinase; AKT: protein kinase B; NF-κB: nuclear factor-kappa B. aP < 0.05 versus the control group, bP < 0.05 versus the model group.
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