Journal of Traditional Chinese Medicine ›› 2024, Vol. 44 ›› Issue (3): 468-477.DOI: 10.19852/j.cnki.jtcm.20240402.001
• Original articles • Previous Articles Next Articles
ZHU Wenting1, GUO Changqing2(), DU Mei2, MA Yunxuan2, CUI Yongqi2, CHEN Xilin2, GUO Changqing3
Received:
2023-08-11
Accepted:
2023-12-19
Online:
2024-06-15
Published:
2024-04-02
Contact:
Mrs. GUO Changqing, Shijiazhuang Medical College, Hebei 050599, China. Supported by:
ZHU Wenting, GUO Changqing, DU Mei, MA Yunxuan, CUI Yongqi, CHEN Xilin, GUO Changqing. Acupotomy alleviates knee osteoarthritis in rabbit by regulating chondrocyte mitophagy via Pink1-Parkin pathway[J]. Journal of Traditional Chinese Medicine, 2024, 44(3): 468-477.
Item | Group | n | Before intervention | After intervention |
---|---|---|---|---|
PROM | CON | 6 | 141.2±2.1 | 140.7±3.5 |
KOA | 6 | 31.2±6.5a | 53.2±10.0a | |
Acu | 6 | 40.3±4.6a | 97.3±12.5ab | |
Lequesne MG | CON | 6 | 0.0±0.0 | 0.0±0.0 |
KOA | 6 | 7.3±0.5 | 6.5±1.0 | |
Acu | 6 | 7.3±0.8 | 3.3±0.8b |
Table 1 Behavioral assessment of rabbits from the 3 groups (scores, $\bar{x} \pm s$)
Item | Group | n | Before intervention | After intervention |
---|---|---|---|---|
PROM | CON | 6 | 141.2±2.1 | 140.7±3.5 |
KOA | 6 | 31.2±6.5a | 53.2±10.0a | |
Acu | 6 | 40.3±4.6a | 97.3±12.5ab | |
Lequesne MG | CON | 6 | 0.0±0.0 | 0.0±0.0 |
KOA | 6 | 7.3±0.5 | 6.5±1.0 | |
Acu | 6 | 7.3±0.8 | 3.3±0.8b |
Figure 1 Cartilage degeneration under microscope A: safranin O-fast green staining of the knee cartilage (× 200); A1: CON group; A2: KOA group; A3: Acu group; B: Col-Ⅱ immunofluorescence staining in knee cartilage (× 200); B1-B3: CON group; B4-B6: KOA group; B7-B9: Acu group; C: analysis of the Mankin score; D: analysis of the Col-Ⅱ fluorescence intensity. CON group: no modelling or no intervention; KOA group: 6 weeks of modeling followed by no intervention; Acu group: 6 weeks of modeling followed by acupotomy intervention for 4 weeks. Col-Ⅱ: type Ⅱ collagen; CON: control; KOA: knee osteoarthritis; Acu: acupotomy. The data were presented as the mean ± standard deviation (n = 6). Compared with the CON group, aP < 0.01; compared with the KOA group, bP < 0.01.
Figure 2 Ultramicrostructure of chondrocyte mitochondria A, B, C: chondrocytes at low magnification (× 1200); D, E, F: chondrocytes at high magnification (× 8000); A, D: CON group; B, E: KOA group; C, F: Acu group; CON group: no modeling or no intervention; KOA group: 6 weeks of modeling followed by no intervention; Acu group: 6 weeks of modeling followed by acupotomy intervention for 4 weeks. The yellow arrow indicates mitochondria; the red arrow indicates mitochondrial autophagosomal vesicles.
Figure 3 Immunofluorescence staining of mitophagy and mitochondrial function A: double immunofluorescence staining for TOM20 and LC3B (× 400); A1, A2, A3, A4: CON group; A5, A6, A7, A8: KOA group; A9, A10, A11, A12: Acu group; A1, A5, A9: DAPI staining; A2, A6, A10: TOM20 staining; A3, A7, A11: LC3B staining; A4, A8, A12: merge of TOM20 and LC3B staining; B: level of ATP; C: colocalization coefficient; D, E: analysis of TOM20 and LC3B fluorescence intensity. CON group: no modeling or no intervention; KOA group: 6 weeks of modeling followed by no intervention; Acu group: 6 weeks of modeling followed by acupotomy intervention for 4 weeks. ATP: adenosine triphosphate; PCC: Pearson's correlation coefficient; TOM20: translocase of the outer membrane 20; LC3B: microtubule-associated protein-1 light chain-3B; CON: control; KOA: knee osteoarthritis; Acu: acupotomy; DAPI: 4',6-diamidino-2-phenylindole. The data were presented as the mean ± standard deviation (n = 6). aP < 0.01 compared with the control group; bP < 0.05, cP < 0.01, compared with the KOA group.
Figure 4 Expression of factors associated with the Pink1-Parkin pathway A: double immunofluorescence staining for Pink1 and Parkin (× 400); A-1A4: CON group; A5-A8: KOA group; A9-A12: Acu group; A1, A5, A9: DAPI staining; A2, A6, A10: Pink1 staining; A3, A7, A11: Parkin staining; A4, A8, A12: merge of Pink1 and Parkin staining; B: Pink1, Parkin, LC3Ⅱ/Ⅰ, TOM20 and TIM23 expression was detected by Western blot analyses. C: colocalization coefficient; D: mRNA expression of Pink1; E: mRNA expression of Parkin; F: Western blotting analysis of Pink1 expression; G: Western blotting analysis of Parkin expression; H: Western blotting analysis of LC3Ⅱ/Ⅰ expression; I: Western blotting analysis of TOM20 expression; J: Western blotting analysis of TIM23 expression. CON group: no modeling or no intervention; KOA group: 6 weeks of modeling followed by no intervention; Acu group: 6 weeks of modeling followed by acupotomy intervention for 4 weeks. PCC: Pearson's correlation coefficient; Pink1: PTEN-induced putative kinase 1; LC3Ⅱ/Ⅰ: microtubule-associated protein-1 light chain-3Ⅱ/Ⅰ; TOM20: translocase of the outer membrane 20; TIM23: translocase of the inner membrane 23; CON: control; KOA: knee osteoarthritis; Acu: acupotomy; DAPI: 4', 6-diamidino-2-phenylindole; PTEN: phosphatase and Tensin Homolog. The data were presented as the mean ± standard deviation (n = 6). aP < 0.01 and dP < 0.01 compared with the control group; bP < 0.01 and cP < 0.05, compared with the KOA group.
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