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Journal of Traditional Chinese Medicine ›› 2021, Vol. 41 ›› Issue (5): 677-683.DOI: 10.19852/j.cnki.jtcm.2021.05.003

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Mechanism of total glucosides from Chishao(Radix Paeoniae Rubra)on proliferation and apoptosis of hepatocellular carcinoma cells via phosphatase and tensin homolog deleted on chromosome ten/phosphatidylinositol 3-kinase/protein kinase B signaling pathway

FAN Bingbing, LI Tianjiao, MENG Xiansheng, WANG Shuai, BAO Yongrui, WANG Fei   

  1. College of Pharmacy, Liaoning University of Traditional Chinese Medicine;College of Graduate Studies, Liaoning University of Traditional Chinese Medicine;
  • Online:2021-10-12 Published:2021-10-15
  • Supported by:
    Supported by National Natural Science Foundation of China:Study on the Method of Discovering Active Substance in Anti-liver Cancer of Oroxylum Indicum Based on Microfluidic Cell Biological Chip Technology (No. 81874342), Supported by National Key R&D Program of China:Take Bufei Jianpi Formula as a Model to Study the Material Basis and Mechanism of Improving COPD (SQ2018YFC170161), the Project of Pnnovation Team of Liaoning Province:Innovative Team of Integrated Research on Pharmacodynamic Metabonomics and Mechanism of Traditional Chinese Medicine (No.LT2017015)

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Abstract: OBJECTIVE: To investigate the possible molecular mechanism of total glycosides of Chishao(Radix Paeoniae Rubra)(TG-RPR) on proliferation and apoptosis of hepatocellular carcinoma cells.METHODS: The proliferation of TG-RPR on Hep G2 cells was detected using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay. The apoptosis of Hep G2 cells was measured by annexin V-FITC/double staining. The phosphatase and tensin homolog deleted on chromosome ten(PTEN)/phosphatidylinositol 3-kinase(PI3 K)/protein kinase B(Akt) signaling pathway was evaluated by Western Blot and reverse transcription-polymerase chain reaction(RT-PCR).RESULTS: TG-RPR can up-regulation the expression of pro-apoptotic factors such as PTEN and BCL2-Associated X(Bax), down-regulation the expression of anti-apoptotic factors including B-cell lymphoma-2(Bcl-2), PI3 K, and Akt.CONCLUSION: TG-RPR significantly inhibits the proliferation of Hep G2 cells in a dose-dependent manner and promotes apoptosis. These results demonstrated TG-RPR has significant inhibitory effect on Hep G2 cells. These results identify a critical role of TG-RPR in proliferation and apoptosis of Hep G2 cells via modulating PTEN/PI3 K/Akt signaling pathway. TG-RPR may offer a promise as a potential pharmaceutical therapy for hepatocellular carcinoma.

Key words: carcinoma,hepatocellular, glycosides, PTEN phosphohydrolase, phosphatidylinositol 3-kinase, proto-oncogene proteins c-akt, Chishao(Radix Paeoniae Rubra)

Cite this article

FAN Bingbing, LI Tianjiao, MENG Xiansheng, WANG Shuai, BAO Yongrui, WANG Fei. Mechanism of total glucosides from Chishao(Radix Paeoniae Rubra)on proliferation and apoptosis of hepatocellular carcinoma cells via phosphatase and tensin homolog deleted on chromosome ten/phosphatidylinositol 3-kinase/protein kinase B signaling pathway[J]. Journal of Traditional Chinese Medicine, 2021, 41(5): 677-683.