Journal of Traditional Chinese Medicine ›› 2023, Vol. 43 ›› Issue (1): 78-86.DOI: 10.19852/j.cnki.jtcm.20220727.001
• Original articles • Previous Articles Next Articles
TIAN Meijing1, HE Yannan1, ZHENG Mingcui1, QIN Gaofeng3, GONG Zhuoyan1, HUANG Shuaiyang2(), WANG Pengwen1()
Received:
2021-12-12
Accepted:
2022-03-26
Online:
2023-02-15
Published:
2023-01-10
Contact:
HUANG Shuaiyang,WANG Pengwen
About author:
Dr. HUANG Shuaiyang, Department of Respiratory Medicine, the Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China. 1138507954@qq.com. Telephone:+86-10-84013195; +86-18813110509Supported by:
TIAN Meijing, HE Yannan, ZHENG Mingcui, QIN Gaofeng, GONG Zhuoyan, HUANG Shuaiyang, WANG Pengwen. Chinese herbal compound Jinsiwei (金思维) improves synaptic plasticity in mice with sporadic Alzheimer’s disease induced by streptozotocin[J]. Journal of Traditional Chinese Medicine, 2023, 43(1): 78-86.
Figure 1 Efficacy of Jinsiwei on Morris Water Maze test and step-down passive avoidance test results in STZ-induced SD mice A: escape latency; B: swimming distance; C: number of crossing the platform; D: error times; E: step-down latency. CG: control group (mice were administered with an equal volume of 0.5% carboxymethyl cellulose via oral gavage once a day for 3 months); MG: model group (mice were administered with an equal volume of 0.5% carboxymethyl cellulose via oral gavage once a day for 3 months); DG: donepezil group (mice were administered with 0.92 mg/kg donepezil via oral gavage once a day for 3 months); JH: Jinsiwei high-dose group (mice were administered with 20 mg/kg Jinsiwei via oral gavage once a day for 3 months); JM: Jinsiwei medium-dose group (mice were administered with 10 mg/kg Jinsiwei via oral gavage once a day for 3 months); JL: Jinsiwei low-dose group (mice were administered with 5 mg/kg Jinsiwei via oral gavage once a day for 3 months). STZ: streptozotocin; SAD: sporadic Alzheimer’s disease. Results are shown as mean ± standard deviation (n = 15). aP < 0.01, model group vs control group; bP < 0.01, donepezil group vs model group; dP < 0.05, Jinsiwei high-dose group vs model group; eP < 0.01, fP < 0.05, Jinsiwei medium-dose group vs model group; cP < 0.01, gP < 0.05, Jinsiwei low-dose group vs model group.
Figure 2 Efficacy of Jinsiwei on the number of synapses in STZ-induced SAD mice A: ultrastructure of synapses in the hippocampal CA1 of mice were observed under transmission electron microscopy at ×2.0 k; B: number of synapses in hippocampal CA1 regions; →: synapses; scale bar = 2 μm. A1: control group (mice were administered with an equal volume of 0.5% carboxymethyl cellulose via oral gavage once a day for 3 months); A2: model group (mice were administered with an equal volume of 0.5% carboxymethyl cellulose via oral gavage once a day for 3 months); A3: donepezil group (mice were administered with 0.92 mg/kg donepezil via oral gavage once a day for 3 months); A4: Jinsiwei high-dose group (mice were administered with 20 mg/kg Jinsiwei via oral gavage once a day for 3 months); A5: Jinsiwei medium-dose group (mice were administered with 10 mg/kg Jinsiwei via oral gavage once a day for 3 months); A6: Jinsiwei low-dose group (mice were administered with 5 mg/kg Jinsiwei via oral gavage once a day for 3 months); STZ: streptozotocin; SAD: sporadic Alzheimer’s disease. Results are shown as mean ± standard deviation (n = 3). aP < 0.01 vs control group, bP < 0.01, cP < 0.05 vs model group.
Figure 3 Efficacy of Jinsiwei on the distribution of positive cells and protein expression of drebrin and cofilin in the hippocampal CA1 region of STZ-induced SAD mice A, B: distribution and positive cells of drebrin in the hippocampal CA1 region of mice (immunohistochemical staining, ×20); C, D: distribution and positive cells of cofilin in the hippocampal CA1 region of mice (immunohistochemical staining, ×20); E: protein expression of drebrin; F: protein expression of cofilin; →: positive cells of cofilin; scale bar = 50 μm. A1, C1, CG: control group (mice were administered with an equal volume of 0.5% carboxymethyl cellulose via oral gavage once a day for 3 months); A2, C2, MG: model group (mice were administered with an equal volume of 0.5% carboxymethyl cellulose via oral gavage once a day for 3 months); A3, C3, DG: donepezil group (mice were administered with 0.92 mg/kg donepezil via oral gavage once a day for 3 months); A4, C4, JH: Jinsiwei high-dose group (mice were administered with 20 mg/kg Jinsiwei via oral gavage once a day for 3 months); A5, C5, JM: Jinsiwei medium-dose group (mice were administered with 10 mg/kg Jinsiwei via oral gavage once a day for 3 months); A6, C6, JL: Jinsiwei low-dose group (mice were administered with 5 mg/kg Jinsiwei via oral gavage once a day for 3 months); STZ: streptozotocin; SAD: sporadic Alzheimer’s disease. Results are shown as mean ± standard deviation (n = 6). aP < 0.01 vs control group; bP < 0.01, cP < 0.05 vs model group.
Figure 4 Efficacy of Jinsiwei on the distribution of positive cells and protein expression of syn and NR2B in the hippocampal CA1 region of STZ-induced SAD mice A, B: distribution and positive cells of syn in the hippocampal CA1 region of mice (immunohistochemical staining, ×20); C, D: distribution and positive cells of NR2B in the hippocampal CA1 region of mice (immunohistochemical staining, ×20); E: protein expression of syn; F: protein expression of NR2B; →: positive cells of syn; scale bar = 50 μm. A1, C1, CG: control group (mice were administered with an equal volume of 0.5% carboxymethyl cellulose via oral gavage once a day for 3 months); A2, C2, MG: model group (mice were administered with an equal volume of 0.5% carboxymethyl cellulose via oral gavage once a day for 3 months); A3, C3, DG: donepezil group (mice were administered with 0.92 mg/kg donepezil via oral gavage once a day for 3 months); A4, C4, JH: Jinsiwei high-dose group (mice were administered with 20 mg/kg Jinsiwei via oral gavage once a day for 3 months); A5, C5, JM: Jinsiwei medium-dose group (mice were administered with 10 mg/kg Jinsiwei via oral gavage once a day for 3 months); A6, C6, JL: Jinsiwei low-dose group (mice were administered with 5 mg/kg Jinsiwei via oral gavage once a day for 3 months). NR2B: N-methyl D-aspartate receptor subtype 2B; STZ: streptozotocin; SAD: sporadic Alzheimer’s disease. Results are shown as mean ± standard deviation (n = 6). aP < 0.01 vs control group; bP < 0.01, cP < 0.05 vs model group.
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