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Journal of Traditional Chinese Medicine ›› 2019, Vol. 39 ›› Issue (05): 667-677.DOI: 10.19852/j.cnki.jtcm.2019.05.008

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Shen-Zhi-Ling oral solution improves learning and memory ability in Alzheimer's disease mouse model

Chi Huiying, Liu Te, Pan Weidong, Chen Jiulin, Wu Beiling, Yu Zhihua, Chen Chuan   

  1. Shanghai Geriatric Institute of Chinese Medicine, Shanghai University of Traditional Chinese Medicine;Department of Pathology, Yale University School of Medicine;Department of Neurology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine;
  • Online:2019-10-15 Published:2019-10-15
  • Supported by:
    Supported by Shanghai Natural Science Foundation(No.16ZR1434000), Grant from the Shanghai Municipal Health Bureau Fund(No.20134331,20134y014), Grant from the Projects Sponsored by the Development Fund for Shanghai talents(No.2017054), the Projects Sponsored by the Fund for Xinglin Talents of Shanghai University of TCM(201707081)

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Abstract: OBJECTIVE: To investigate the effector mechanisms and effector targets of Shen-Zhi-Ling(SZL)oral solution in the treatment of Alzheimer's disease(AD).METHODS: In this study, we carried out gavage with SZL oral solution in an APP/PS-1 heterozygous double transgenic AD mouse model for 12 continuous weeks.Haematoxylin and eosin staining, Nissl staining and Annexin V/Propidium Iodide staining were used to detect the brain histopathology in AD mouse model.Immunofluorescence staining was used to detect the expression levels of autophagy's proteins.Morris water maze test was used to detect the learning and memory ability in AD mouse model.RESULTS: Pathological results showed that neuronal loss in the hippocampus of mice in the SZL intervention group was significantly alleviated and the number of apoptotic neurons was significantly decreased compared with the control group(physiological saline and non-intervention groups).Immunofluorescence staining results showed that the expression of autophagy activators, Beclin-1 and LC3 B, was significantly increased in the hippocampal neurons of mice of the SZL intervention group,while the expression of the apoptotic factor, caspase-3, was significantly decreased.At the same time, hippocampal accumulation of Aβ42 protein was significantly decreased.In addition, results of the water maze experiment showed that the latency period in mice from the SZL intervention group was significantly reduced.CONCLUSION: In summary, we believe that the SZL oral solution significantly activates autophagy in hippocampal neurons, effectively reducing the accumulation of Aβ42 peptides, alleviating neuronal injury and apoptosis, and ultimately improving the cognitive function in a mouse model of AD.

Key words: Alzheimer disease, Autophagy, Shen-Zhi-Ling oral solution

Cite this article

Chi Huiying, Liu Te, Pan Weidong, Chen Jiulin, Wu Beiling, Yu Zhihua, Chen Chuan. Shen-Zhi-Ling oral solution improves learning and memory ability in Alzheimer's disease mouse model[J]. Journal of Traditional Chinese Medicine, 2019, 39(05): 667-677.