Journal of Traditional Chinese Medicine ›› 2022, Vol. 42 ›› Issue (3): 364-371.DOI: 10.19852/j.cnki.jtcm.20220225.002
• Research Article • Previous Articles Next Articles
Efficacy of Kushen decoction (苦参汤) on high-fat-diet-induced hyperlipidemia in rats
ZHANG Jiri Mutu1,2, LIANG Shilong1, NIE Peng1, LIAO Yong’an1, AI Qinying1, YAN Xiaojun1, LIU Hongning1, JI Yanhua1(
), ZENG Zhijun1()
- 1 Research Center for Differention and Development of TCM Basic Theory, Jiangxi Province Key Laboratory of TCM Etiopathogenisis, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi 330006, China
2 Mongolian Medical College, Inner Mongolia Minzu Uaniversity, Tongliao 028000, China
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Accepted:2021-11-17Online:2022-06-15Published:2022-02-25 -
Contact:JI Yanhua,ZENG Zhijun -
About author:JI Yanhua and ZENG Zhijun, Research Center for Differention and Development of TCM Basic Theory, Jiangxi Province Key Laboratory of TCM Etiopathogenisis, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi 330006, China. 20162018@jxutcm.edu.cn and zhijunzeng@aliyun.com
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Supported by:Supported by National Natural Science Foundation of China(81760787);China Postdoctoral Science Foundation(2017M622101);Scientific Foundation of the Education Department of Jiangxi Province(GJJ190643);Traditional Chinese Medicine Scientific Research Subject of Jiangxi Province(2019A111);Traditional Chinese Medicine Scientific Research Subject of Jiangxi Province(2019B110);Health and Family Planning Commission of Jiangxi Province(20195651);Health and Family Planning Commission of Jiangxi Province(20203766);Health and Family Planning Commission of Jiangxi Province(20203773);Health and Family Planning Commission of Jiangxi Province(202131047);Health and Family Planning Commission of Jiangxi Province(202131054);Collaborative Innovation of Modern Science and Technology and Industrial Development of Jiangxi National Traditional Medicine(Gan Jiao Gao Zi, [2013] No. 109);Jiangxi middle-aged and young backbone Talents Project of traditional Chinese medicine(Gan TCM Ke Jiao Zi [2021] No. 2);Specialized Research Fund for the First-Class Discipline of Chinese Herb from Jiangxi Province(JXSYLXK-ZHYAO111);Key Discipline Project of Jiangxi University of Chinese Medicine(2013jzzdxk069)
Cite this article
ZHANG Jiri Mutu, LIANG Shilong, NIE Peng, LIAO Yong’an, AI Qinying, YAN Xiaojun, LIU Hongning, JI Yanhua, ZENG Zhijun. Efficacy of Kushen decoction (苦参汤) on high-fat-diet-induced hyperlipidemia in rats[J]. Journal of Traditional Chinese Medicine, 2022, 42(3): 364-371.
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Figure 1 Effect of high-fat diet and treatment with low- or high-dose Kushen (A-F) Different parameters were compared between the control group, the untreated model group (high-fat diet), the model group treated with low-dose Kushen decoction (LDKS), and the model group treated with high-dose decoction (HDKS). (A) body weight; (B) body length; (C) Lee’s index; (D) serum high-density lipoprotein cholesterol (HDL-C); (E) serum low-density lipoprotein cholesterol (LDL-C); and (F) serum triglycerides (TG). Compared with the control group, a high-fat diet significantly increased body weight, body length, Lee’s index, and serum TG (aP < 0.01). Compared with the model group, low-dose Kushen decoction (LDKS group) tended to decrease body weight, body length, and Lee’s index, but the differences were not significant (P > 0.05), while the levels of serum HDL-C (cP < 0.05) and serum LDL-C (bP < 0.01) increased significantly, and the level of serum TG decreases significantly (bP < 0.01). Compared with the model group, high-dose Kushen decoction (HDKS group) decreased significantly body weight, Lee’s index, and serum TG (bP < 0.01), while serum HDL-C and serum LDL-C increased significantly (bP < 0.01).
Figure 1 Effect of high-fat diet and treatment with low- or high-dose Kushen (A-F) Different parameters were compared between the control group, the untreated model group (high-fat diet), the model group treated with low-dose Kushen decoction (LDKS), and the model group treated with high-dose decoction (HDKS). (A) body weight; (B) body length; (C) Lee’s index; (D) serum high-density lipoprotein cholesterol (HDL-C); (E) serum low-density lipoprotein cholesterol (LDL-C); and (F) serum triglycerides (TG). Compared with the control group, a high-fat diet significantly increased body weight, body length, Lee’s index, and serum TG (aP < 0.01). Compared with the model group, low-dose Kushen decoction (LDKS group) tended to decrease body weight, body length, and Lee’s index, but the differences were not significant (P > 0.05), while the levels of serum HDL-C (cP < 0.05) and serum LDL-C (bP < 0.01) increased significantly, and the level of serum TG decreases significantly (bP < 0.01). Compared with the model group, high-dose Kushen decoction (HDKS group) decreased significantly body weight, Lee’s index, and serum TG (bP < 0.01), while serum HDL-C and serum LDL-C increased significantly (bP < 0.01).
Figure 2 Analysis of ARNTL, CLOCK, and PER3 mRNAs by real-time fluorescent quantitative polymerase chain reaction Bar graphs showing ARNTL (A), CLOCK (B), and PER3 (C) mRNA levels in the different groups. Model group compare with the control group, aP < 0.05; LDKS group and HDKS group compare with the model group, bP < 0.01 or cP < 0.05.
Figure 2 Analysis of ARNTL, CLOCK, and PER3 mRNAs by real-time fluorescent quantitative polymerase chain reaction Bar graphs showing ARNTL (A), CLOCK (B), and PER3 (C) mRNA levels in the different groups. Model group compare with the control group, aP < 0.05; LDKS group and HDKS group compare with the model group, bP < 0.01 or cP < 0.05.
Figure 3 Quantification of the proteins encoded by the DEGs in livers from the different experimental groups Representative immunoblots assessing the target proteins are shown. Molecular weight markers are indicated in kilodaltons (kDa). Protein levels were normalized to internal controls. β-actin was used as a loading control. Bar charts showing ARNTL (C), CLOCK (D), and PER3 (E) protein expression in the different groups. DEGs: differentially expressed genes; LDKS: low-dose Kushen decoction; HDKS: high-dose decoction. Compared with the control group, the expression of ARNTL, PER3, and CLOCK in the model group was decreased significantly (aP < 0.01 versus control group). It can be noted that the expression of ARNTL, PER3, and CLOCK was increased significantly in the LDKS or HDKS group (bP < 0.05 and cP < 0.01 versus model group).
Figure 3 Quantification of the proteins encoded by the DEGs in livers from the different experimental groups Representative immunoblots assessing the target proteins are shown. Molecular weight markers are indicated in kilodaltons (kDa). Protein levels were normalized to internal controls. β-actin was used as a loading control. Bar charts showing ARNTL (C), CLOCK (D), and PER3 (E) protein expression in the different groups. DEGs: differentially expressed genes; LDKS: low-dose Kushen decoction; HDKS: high-dose decoction. Compared with the control group, the expression of ARNTL, PER3, and CLOCK in the model group was decreased significantly (aP < 0.01 versus control group). It can be noted that the expression of ARNTL, PER3, and CLOCK was increased significantly in the LDKS or HDKS group (bP < 0.05 and cP < 0.01 versus model group).
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