Journal of Traditional Chinese Medicine ›› 2024, Vol. 44 ›› Issue (6): 1187-1193.DOI: 10.19852/j.cnki.jtcm.2024.06.007
• Research Articles • Previous Articles Next Articles
Untargeted serum metabonomic reveals alleviated ovalbumin-induced asthma by Baijin Pingchuan (白金平喘) through primary bile acid biosynthesis
DING Lizhong, ZHANG Qiang, SUN Yingying, KONG Yibu, SONG Yongfu, WANG Yongji(
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- Department of Pediatrics, the Affiliated hospital to Changchun University of Chinese Medicine, Changchun 130017, China
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Received:2023-07-22Accepted:2023-11-27Online:2024-12-15Published:2024-11-12 -
Contact:WANG Yongji, Department of Pediatrics, the Affiliated hospital to Changchun University of Chinese Medicine, Changchun 130017, China. wangyjcczyy@163.com Telephone: +86-431-81953806 -
Supported by:Science and Technology Development Plan Project of Jilin Provincial: Research on the Development of Baijin Pingchuan Granules as an In-hospital Preparation for the Treatment of Pediatric Asthma (Heat-type Asthma)(20200404043YY);Traditional Chinese Medicine Science and Technology Project of Jilin Provincial: Exploring the Mechanism of Baijin Pingchuan Granules in Alleviating Airway Inflammation in Asthma based on the “Three-Stage Differentiation and Treatment” Theory of National Medical Masters(2020090)
Cite this article
DING Lizhong, ZHANG Qiang, SUN Yingying, KONG Yibu, SONG Yongfu, WANG Yongji. Untargeted serum metabonomic reveals alleviated ovalbumin-induced asthma by Baijin Pingchuan (白金平喘) through primary bile acid biosynthesis[J]. Journal of Traditional Chinese Medicine, 2024, 44(6): 1187-1193.
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Figure 1 Performance score of asthma rats in the control, OVA, DEX, low-, median-, and high-dose baijinpingchuan groups The rats in the control group treated with saline, the OVA group treated with 1% OVA, DEX treated with dexame-thasone (0.5 mg/kg), low-dose treated with BJPC (90 g/kg), median-dose treated with BJPC (160 g/kg), high-dose treated with BJPC (320 g/kg). All the groups were treated for 7 d; The two-tailed paired Student’s t-test was used to compare data among these groups, and data was expressed using mean?±?standard deviation (n = 10). OVA: ovalbumin; DEX: dexamethasone; BJPC: Baijin Pingchuan. aP < 0.0001, compared with the control group; bP < 0.01, compared with DEX group.
Figure 1 Performance score of asthma rats in the control, OVA, DEX, low-, median-, and high-dose baijinpingchuan groups The rats in the control group treated with saline, the OVA group treated with 1% OVA, DEX treated with dexame-thasone (0.5 mg/kg), low-dose treated with BJPC (90 g/kg), median-dose treated with BJPC (160 g/kg), high-dose treated with BJPC (320 g/kg). All the groups were treated for 7 d; The two-tailed paired Student’s t-test was used to compare data among these groups, and data was expressed using mean?±?standard deviation (n = 10). OVA: ovalbumin; DEX: dexamethasone; BJPC: Baijin Pingchuan. aP < 0.0001, compared with the control group; bP < 0.01, compared with DEX group.
Figure 2 Expression of IL-25, IL-33, TSLP, and TGF-β1 detected using ELISA A: IL-25; B: IL-33; C: TSLP; D: TGF-β1. The rats in the control group treated with saline, the OVA group treated with 1% OVA, DEX treated with dexamethasone (0.5 mg/kg), low-dose treated with BJPC (90 g/kg), median-dose treated with BJPC (160 g/kg), high-dose treated with BJPC (320 g/kg). All the groups were treated for 7 d. IL-25: interleukin-25; IL-33: interleukin-33; TSLP: thymic stromal lymphopoietin; TGF-β1: transforming growth factor-beta 1; ELISA: enzyme-linked immunosorbent assay. OVA: ovalbumin; DEX: dexamethasone. The two-tailed paired Student’s t-test was used to compare data among these groups, and data was expressed using mean?±?standard deviation (n = 10). aP < 0.0001, compared with the control group; bP < 0.01, compared with DEX group.
Figure 2 Expression of IL-25, IL-33, TSLP, and TGF-β1 detected using ELISA A: IL-25; B: IL-33; C: TSLP; D: TGF-β1. The rats in the control group treated with saline, the OVA group treated with 1% OVA, DEX treated with dexamethasone (0.5 mg/kg), low-dose treated with BJPC (90 g/kg), median-dose treated with BJPC (160 g/kg), high-dose treated with BJPC (320 g/kg). All the groups were treated for 7 d. IL-25: interleukin-25; IL-33: interleukin-33; TSLP: thymic stromal lymphopoietin; TGF-β1: transforming growth factor-beta 1; ELISA: enzyme-linked immunosorbent assay. OVA: ovalbumin; DEX: dexamethasone. The two-tailed paired Student’s t-test was used to compare data among these groups, and data was expressed using mean?±?standard deviation (n = 10). aP < 0.0001, compared with the control group; bP < 0.01, compared with DEX group.
Figure 3 Observation for the pathological structure of the lung using HE staining in each group (×100) A: control; B: DEX treatment; C: OVA treatment; D: low-dose BJPC treatment; E: median-dose BJPC treatment; F: high-dose BJPC treatment. The rats in the control group treated with saline, the OVA group treated with 1% OVA, DEX treated with dexamethasone (0.5 mg/kg), low-dose treated with BJPC (90 g/kg), median-dose treated with BJPC (160 g/kg), high-dose treated with BJPC (320 g/kg). All the groups were treated for 7 d (n = 10). HE: hematoxylin-eosin; OVA: ovalbumin; DEX: dexamethasone; BJPC: Baijin Pingchuan.
Figure 3 Observation for the pathological structure of the lung using HE staining in each group (×100) A: control; B: DEX treatment; C: OVA treatment; D: low-dose BJPC treatment; E: median-dose BJPC treatment; F: high-dose BJPC treatment. The rats in the control group treated with saline, the OVA group treated with 1% OVA, DEX treated with dexamethasone (0.5 mg/kg), low-dose treated with BJPC (90 g/kg), median-dose treated with BJPC (160 g/kg), high-dose treated with BJPC (320 g/kg). All the groups were treated for 7 d (n = 10). HE: hematoxylin-eosin; OVA: ovalbumin; DEX: dexamethasone; BJPC: Baijin Pingchuan.
Figure 4 Differential metabolites were identified between the OVA and high-dose BJPC groups A: volcano map; B: heatmap. The rats the OVA group treated with 1% OVA, GND treated with BJPC (320 g/kg). All the groups were treated for 7 d (n = 9). BJPC: Baijin Pingchuan; GND: high-dose BJPC group; MX: ovalbumin.
Figure 4 Differential metabolites were identified between the OVA and high-dose BJPC groups A: volcano map; B: heatmap. The rats the OVA group treated with 1% OVA, GND treated with BJPC (320 g/kg). All the groups were treated for 7 d (n = 9). BJPC: Baijin Pingchuan; GND: high-dose BJPC group; MX: ovalbumin.
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