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Journal of Traditional Chinese Medicine ›› 2022, Vol. 42 ›› Issue (3): 451-457.DOI: 10.19852/j.cnki.jtcm.20220408.004

• Research Article • Previous Articles     Next Articles

Sterile alpha motif and histidine-aspartic acid domain-containing protein 1 expression and its relationship with T cell activation in human immunodeficiency virus/acquired immune deficiency syndrome patients with lung-spleen Qi deficiency syndrome pattern

LIU Zhen1, ZHANG Qingyan1, DENG Bowen1, SANG Feng1, WANG Danni2, KANG Ning3, LI Jie1, ZHANG Min2, LIANG Shoupei4, DUAN Chenchen1, LIU Zhibin2, XU Qianlei2(), LI Qiang1()   

  1. 1 Henan Key Laboratory of Viral Diseases Prevention and Treatment of Traditional Chinese Medicine, the First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450000, China
    2 Department of Acquired Immune Deficiency Syndrome Treatment and Research Center, The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450000, China
    3 Department of Obstetrics and Gynecology, the First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450000, China
    4 Medical Department, Medical Department of Zhengzhou People’s Hospital, Zhengzhou 450000, China
  • Received:2021-04-26 Accepted:2021-07-08 Online:2022-06-15 Published:2022-04-08
  • Contact: XU Qianlei,LI Qiang
  • About author:XU Qianlei, Department of Acquired Immune Deficiency Syndrome Treatment and Research Center, the First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450000, China. xuqianlei666@126.com;
  • Supported by:
    National Natural Science Foundation of China (Construction of HIV Pseudovirus Infected Cell Model and Application of Yiaikang in the Study of T Cell Immune Response)(82004207);National Special Science and Technology Program on Major Infectious Diseases (Study on the Scheme of TCM to Reduce the Incidence of Adverse Reactions to AIDS Antiviral Therapy)(2017ZX10205502002);Study on the Scheme of TCM to Reduce the Incidence of AIDS Opportunistic Infection(2017ZX10205502003);Science and Technology Tackling Plan of Henan Province (Exploring the Activation Mechanism of Yiaikang on CD8+ T Lymphocytes in HIV/AIDS Patients based on STING/SAMHD1 Pathway)(202102310178);Regulation of HIV/AIDS Treg Cell-Derived Exosomeso on Activation of CD4+ T Cells and the Intervention of Yiaikang(212102311126);Subject Construction Project of Henan Characteristic Backbone Subject of TCM of Henan University of TCM (Deep Sequencing/Molecular Docking/Gene Editing Network System to Analyze The Mechanism of TCM Regulating the Immune Function of HIV Patients)(STG-ZYXKY-2020029);Henan Province TCM Research Project (Explore the Mechanism of Yiaikang in the Treatment of AIDS Immunity Based on HIV gag-CTL Response)(2018JDZX106);Henan Province TCM Research Project (Explore the Mechanism of Yiaikang in the Treatment of AIDS Immunity Based on HIV gag-CTL Response)(2019AZB003);Henan Province TCM Research Project (Study on TCM Treatment of Aged AIDS)(20-21ZYZD03);Key Scientific Research Projects of Colleges and Universities in Henan Province (to Explore the Regulation Mechanism of Yiaikang on AIDS Lung Qi Deficiency Syndrome from TGF-β /Smad/ID)(20A360010)

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Abstract:

OBJECTIVE: To investigate the relationship between antiviral restriction factor Sterile Alpha Motif and Histidine-Aspartic acid domain-containing protein 1 (SAMHD1) expression and T cell activation, furthermore, identifying objective indexes of lung-spleen Qi deficiency symptom pattern.

METHODS: We assessed the profile of T lymphocyte subsets, characteristics of SAMHD1 and human leukocyte antigen DR (HLA-DR) expression in lung-spleen Qi deficiency patients. At the same time, people living with human immunodeficiency virus / acquired immune deficiency syndrome (HIV/AIDS) (PLWHA) without obvious clinical symptoms and healthy donors in this area were used as controls.

RESULTS: Immunohematologic indexes lower CD4 count, lower CD4/CD8 ratio and higher SAMHD1 level were found in lung-spleen Qi deficiency patients. Furthermore, we demonstrated a positive relationship between SAMHD1 and HLA-DR level as well as with interferon factor in lung-spleen Qi deficiency syndrome and patients without obvious clinical signs and symptoms groups.

CONCLUSIONS: These data indicated the positive relationship between SAMHD1 and T cell activation which further elucidated the role of SAMHD1 in cellular immune response. Furthermore, combination of T lymphocyte subsets counts and SAMHD1 level may be used as clinical and biological reference basis for the differentiation and diagnosis of HIV / AIDS traditional Chinese medicine syndromes.

Key words: HIV, acquired immunodeficiency syndrome, symptoms complex, SAM domain and HD domain-containing protein 1, T-lymphocytes

Cite this article

LIU Zhen, ZHANG Qingyan, DENG Bowen, SANG Feng, WANG Danni, KANG Ning, LI Jie, ZHANG Min, LIANG Shoupei, DUAN Chenchen, LIU Zhibin, XU Qianlei, LI Qiang. Sterile alpha motif and histidine-aspartic acid domain-containing protein 1 expression and its relationship with T cell activation in human immunodeficiency virus/acquired immune deficiency syndrome patients with lung-spleen Qi deficiency syndrome pattern[J]. Journal of Traditional Chinese Medicine, 2022, 42(3): 451-457.