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Journal of Traditional Chinese Medicine >

2026 , Vol. 46 >Issue 2: 274 - 284

DOI: https://doi.org/10.19852/j.cnki.jtcm.20251215.001

Original Articles

Qianyang Yuyin granule (潜阳育阴颗粒) modulates glycolysis and inflammatory response to treat hypertensive cardiac remodeling

  • SUN Zeqi ,
  • ZHANG Weiting ,
  • FAN Yadong ,
  • XU Junyao ,
  • HUANG Hong ,
  • XIONG Ziwen ,
  • CAO Huiting ,
  • LIU Ming ,
  • FANG Zhuyuan
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  • 1 Nanjing University of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, China
    2 Institute of Hypertension, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, China
    3 Department of Cardiology, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, China
Prof. FANG Zhuyuan, Department of Cardiology, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, China; Institute of Hypertension, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, China. fangzhuyuan@njucm.edu.cn; Telephone: +86-13951617800;
Prof. LIU Ming, Department of Cardiology, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, China; Institute of Hypertension, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, China. liuming@njucm.edu.cn; Telephone: +86-13815885859

Received date: 2024-12-12

  Accepted date: 2025-06-10

  Online published: 2025-12-15

Supported by

Jiangsu Chinese Medicine Clinical Medicine Innovation Center for Hypertension to Prevention Treatment and Translational Research of Traditional Chinese Medicine for Early Renal Damage in Hypertension(grants k2021j17);Jiangsu Chinese Medicine Clinical Medicine Innovation Center for Hypertension to Prevention Treatment and Translational Research of Traditional Chinese Medicine for Early Renal Damage in Hypertension(k2021j17-1);The Research Innovation Program for College Graduates of Jiangsu Province, to Exploring the Mechanism of Qianyang Yuyin Granule in Improving Hypertensive Renal Damage and Exploring the Mechanism of Yangxin Shumai Granule in Treating Heart Failure with Preserved Ejection Fraction by Attenuating Cardiac Fibrosis(KYCX22_1932);The Research Innovation Program for College Graduates of Jiangsu Province, to Exploring the Mechanism of Qianyang Yuyin Granule in Improving Hypertensive Renal Damage and Exploring the Mechanism of Yangxin Shumai Granule in Treating Heart Failure with Preserved Ejection Fraction by Attenuating Cardiac Fibrosis(SJCX25_1029);The 2023 Youth Fund of Jiangsu Science and Technology Plan Special Project (Investigating the Mechanism of Qianyang Yuyin Granule in Alleviating Hypertensive Renal Vascular Remodeling by Regulating Vascular Smooth Muscle Cell-macrophage Crosstalk via Extracellular Vesicles)(SBK2023045102);The Youth Program of Jiangsu Province Chinese Medicine Science and Technology Development Plan to Exploring the Mechanism of Qianyang Yuyin Granule in Improving Hypertensive Myocardial Remodeling Based on FUN14 Domain-containing Protein 1 Regulation of the Mitophagy-Panoptosis Axis(QN202410)

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Abstract

OBJECTIVE: To investigate the effect of Qianyang Yuyin granule (潜阳育阴颗粒QYYY) on AngII-induced hypertensive cardiac remodeling, focusing on the role of pyruvate kinase isozyme M2 (PKM2) mediated glycolysis.

METHODS: A hypertensive mouse model was established in male C57BL/6 mice by continuous infusion of angiotensin II (AngII; 1000 ng·kg-1·min-1). Mice were administered varying doses of QYYY, with sacubitril/ valsartan (Sac/Val) serving as the positive control. Parameters evaluated included blood pressure, cardiac function, hypertrophy, fibrosis, inflammation, and apoptosis. The metabolic profile of myocardial tissue was analyzed using ultra performance liquid chromatography tandem mass spectrometry. Additionally, the involvement of the hypoxia-inducible factor 1-alpha (HIF-1α)/PKM2 signaling pathway was examined by Western blotting and immunohistochemistry.

RESULTS: QYYY significantly lowered blood pressure, attenuated cardiac hypertrophy and fibrosis, reduced serum levels of inflammatory factors tumor necrosis factor-α and tumor necrosis factor-β, and decreased activation of phospho-NF-kappa B p65 pathway in cardiac tissue of hypertensive mice. Metabolomic analysis indicated that QYYY ameliorated cardiometabolic dysfunction, primarily associated with energy and amino acid metabolism, involving modulation of the HIF-1α/PKM2-mediated glycolytic pathway.

CONCLUSION: QYYY effectively improves cardiac remodeling in hypertensive mice, potentially through inhibition of the PKM2-mediated glycolytic signaling pathway.

Key words： cardiac remodeling; glycolysis; pyruvate kinase isozyme M2; Qianyang Yuyin granule

Cite this article

SUN Zeqi , ZHANG Weiting , FAN Yadong , XU Junyao , HUANG Hong , XIONG Ziwen , CAO Huiting , LIU Ming , FANG Zhuyuan . Qianyang Yuyin granule (潜阳育阴颗粒) modulates glycolysis and inflammatory response to treat hypertensive cardiac remodeling[J]. Journal of Traditional Chinese Medicine, 2026 , 46(2) : 274 -284 . DOI: 10.19852/j.cnki.jtcm.20251215.001

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