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Journal of Traditional Chinese Medicine >

2025 , Vol. 45 >Issue 3: 552 - 560

DOI: https://doi.org/10.19852/j.cnki.jtcm.2025.03.004

Intervention and mechanism of Xiaoyin Anshen Yin (消银安神饮) in treatment of psoriasis combined with sleep disorders

  • Ruohan DIAO ,
  • Xingwu DUAN ,
  • Lingling LI ,
  • Tiange QU ,
  • Huishang FENG ,
  • Guangshan CHEN
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  • 1 Department of Dermatology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
    2 Beijing University of Chinese Medicine, Beijing 100029, China
    3 Department of Dermatology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China

Received date: 2024-10-26

  Accepted date: 2025-01-14

  Online published: 2025-05-21

Supported by

National Natural Science Foundation of China: Study on the Mechanism of Cooling Blood and Tranquilizing Mind in the Treatment of Psoriasis with Sleep Disorder based on the Regulation of Oxidative Stress by Melatonin(82074436)

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Abstract

OBJECTIVE: To explore the therapeutic mechanisms of Xiaoyin Anshen Yin (消银安神饮, XYAS) in treating psoriasis associated with sleep focusing on melatonin and the regulation of the nuclear factor kappa-B (NF-κB) pathway.
METHODS: Forty Sprague-Dawley rats were randomly divided into four groups, and administered distilled water, XYAS and its two different disassembly prescriptions by gavage respectively. Four types of drug-containing serums corresponding to the four groups were then prepared. Tumor necrosis factor (TNF)-α stimulated HaCaT was used to establish a psoriasis cell model, and the serums and the retinoid related orphan receptor alpha (RORα) inverse agonist were used respectively to intervene in the model. Enzyme-linked immunosorbent assay was used to detect the levels of interleukin (IL)-6 and melatonin in each group; flow cytometry was used to detect the levels of reactive oxygen species (ROS), mitochondrial membrane potential, and apoptosis; Western blot was used to evaluate the levels of superoxide dismutase 2 (SOD2), cytochrome-c (Cyt-c), inhibitor of kappa-B alpha (IκBα), p65 and phosphorylated p65.
RESULTS: XYAS and its disassembly prescriptions inhibited the secretion of inflammatory factors such as IL-6, reduced the ROS content and Cyt-c expression, increased the mitochondrial membrane potential and SOD2 content, promoted the apoptosis in HaCaT cells and inhibited the activation of the NF-κB pathway. XYAS was also found increase the melatonin content. The above effects are beneficial in the treatment of psoriasis combined with sleep disorders. Meanwhile, XYAS no longer had a significant ameliorative effect after applying the RORα inverse agonist, suggesting that the therapeutic effect of XYAS is related to RORα.
CONCLUSIONS: The results of this study confirm that XYAS can be utilized for the treatment of psoriasis combined with sleep disorders via inhibiting the NF-κB pathway, anti-inflammatory, antioxidant and pro-apoptotic, which is in part related to the regulatory role of melatonin and its receptor RORα.

Key words： psoriasis; melatonin; nuclear receptor subfamily 1, group F, member 1; NF-kappa B; sleep disorder

Cite this article

Ruohan DIAO , Xingwu DUAN , Lingling LI , Tiange QU , Huishang FENG , Guangshan CHEN . Intervention and mechanism of Xiaoyin Anshen Yin (消银安神饮) in treatment of psoriasis combined with sleep disorders[J]. Journal of Traditional Chinese Medicine, 2025 , 45(3) : 552 -560 . DOI: 10.19852/j.cnki.jtcm.2025.03.004

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