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Journal of Traditional Chinese Medicine >

2025 , Vol. 45 >Issue 3: 508 - 517

DOI: https://doi.org/10.19852/j.cnki.jtcm.2025.03.008

Xiong's Shiwei Wendan decoction (熊氏十味温胆汤) attenuates plaque lesions and balances gut microbiota dysbiosis in ApoE-/- mice with high-fat diet

  • Qian LIU ,
  • Liuchen XIAO ,
  • Yue YUAN ,
  • Xiaopeng DANG ,
  • Jie WEN ,
  • Moye TAN ,
  • Yuxin LIU ,
  • Hongfeng GU ,
  • Xuejiao XIE
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  • 1 Graduate School of Hunan University of Chinese Medicine, Changsha 410208, China
    2 Graduate School of Hunan University of Chinese Medicine, Changsha 410208, China
    3 Department of preventive treatment of disease, Changde First Hospital of Traditional Chinese Medicine, Changde 415099, China
    4 Graduate School of Hunan University of Chinese Medicine, Changsha 410208, China
    5 Department of Zhong jing' Theory, College of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China
    6 Hengyang Key Laboratory of Neurodegeneration and Cognitive Impairment and Institute of Neuroscience, Hengyang Medical College, University of South China, Hengyang 421001, China
    7 Department of Zhong jing' Theory, College of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China

Received date: 2024-02-22

  Accepted date: 2024-09-27

  Online published: 2025-05-21

Supported by

National Natural Science Foundation of China: the Anti-Atherosclerotic Mechanism of Xiong’s Shiwei Wendan decoction based on the Regulation of Reverse Cholesterol Transport via the MicroRNA-33A Adenosine Triphosphate-binding Cassette Subfamily A Member 1/ Adenosine Triphosphate-binding Cassette Subfamily A Member 1 Pathway(81603600);Natural Science Foundation of Hunan Province of China: the Anti-Atherosclerotic Mechanism of Xiong's Shiwei Wendan Decoction based on the Activation of Lipophagy Mediated by the AMP-activated Protein Kinase/Mechanistic Target of Rapamycin Pathway(2021JJ30510);Health Research Project of Hunan Provincial Health Commission: the An-ti-Atherosclerotic Mechanism of Xiong's Shiwei Wendan Decoction based on Lipophagy Mediated by the MicroRNA-499a-3p/ Autophagy-related 5 Pathway(D202303018265)

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Abstract

OBJECTIVE: To evaluate the anti-atherosclerotic potential and gut microbiota (GM) modulation effects of Xiong's Shiwei Wendan decoction (熊氏十味温胆汤, XSWD).
METHODS: For in vitro study, Tsuchiya human peripheral blood mononuclear cell-1 (THP-1) derived foam cells were used to examine the possible anti-atherosclerotic effect of XSWD and XSWD-medicated serum. Atherosclerosis-prone apolipoprotein E-deficient (ApoE-/-) mice were utilized for in vivo analysis. After an 8-week high-fat diet (HFD) adminstration, 25 male ApoE-/- mice were randomly divided into the model group, different doses of XSWD groups (1.25, 2.5, 5 mg/mL), and atorvastatin group (2.6 mg/kg). Following a continuous 8-week intervention, all mice underwent examination for AS lesion formation and assessment of its serum lipid profile. To investigate the effect on the gut microbiome, 16S rRNA gene sequencing targeting the V3-V4 hypervariable region was performed on the colonic content of mice.
RESULTS: XSWD administration attenuated lipid deposition in THP-1 cells, significantly reduced aortic plaque lesions, improved the lipid profile, and normalized GM composition in HFD-fed ApoE-/- mice.
CONCLUSION: This study investigated the potential anti-atherosclerotic and gut microbio-ta-restoring effects of XSWD in ApoE-/- mice, with findings suggesting that XSWD may be a promising preventive measure against atherosclerosis through its ability to reduce lipid accumula-tion in foam cells, improve lipid profile, and restore gut microbiota composition.

Key words： atherosclerosis; lipid metabolism; gastrointestinal microbiome; Xiong's Shiwei Wendan decoction

Cite this article

Qian LIU , Liuchen XIAO , Yue YUAN , Xiaopeng DANG , Jie WEN , Moye TAN , Yuxin LIU , Hongfeng GU , Xuejiao XIE . Xiong's Shiwei Wendan decoction (熊氏十味温胆汤) attenuates plaque lesions and balances gut microbiota dysbiosis in ApoE-/- mice with high-fat diet[J]. Journal of Traditional Chinese Medicine, 2025 , 45(3) : 508 -517 . DOI: 10.19852/j.cnki.jtcm.2025.03.008

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