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Journal of Traditional Chinese Medicine >

2025 , Vol. 45 >Issue 1: 132 - 139

DOI: https://doi.org/10.19852/j.cnki.jtcm.2025.01.012

Difference of the gut microbiota of premature ovarian insufficiency in two traditional Chinese syndromes

  • Jiaman WU ,
  • Yan NING ,
  • Liya TAN ,
  • Fei MA ,
  • Yanting LIN ,
  • Yuanyuan ZHUO
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  • 1 Department of Traditional Chinese Medicine, Shenzhen Maternity and Child Healthcare Hospital, Shenzhen 518028, China
    2 Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China
    3 Department of Acupuncture and Moxibustion, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China

Received date: 2023-08-22

  Accepted date: 2024-04-25

  Online published: 2025-01-10

Supported by

Sanming Project of Medicine in Shenzhen: the First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Luo Songping National Famous Chinese Medicine Practitioner Female Reproductive Disorders Prevention and Treatment Team(SZZYSM202311010);Guangdong Provincial Administration of Traditional Chinese Medicine: Investigation of the Mechanism of Regulating Ren-Tong-Du Acupuncture on Ovarian Granulosa Cells in Polycystic Ovary Syndrome based on Activin A /Smads Signalling Pathway(20181229);Guangdong Provincial Administration of Traditional Chinese Medicine: Evaluation of the Efficacy of Menstrual Regulation and Pregnancy Promotion by Acupuncture in the Treatment of Premature Ovarian Insufficiency(20201294);Shenzhen Science and Innovation Commission: Investigating the Mechanism of Action of Acupuncture in Regulating the Gut Microbiome to Inhibit Apoptosis of Ovarian Granulosa Cells in Premature Ovarian Insufficiency Mice based on the Rictor/Torepamycin Target Protein C2 Pathway(JCYJ20210324130001004)

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Abstract

PURPOSE: To investigate the differences in gut microbial characteristics between two traditional Chinese syndromes of premature ovarian insufficiency (POI).

METHODS: Forty women with POI were recruited from the Department of Traditional Chinese Medicine at Shenzhen Maternity and Child Healthcare Hospital between June and December 2020. Women with POI were divided into the kidney deficiency and blood stasis syndrome (SDBS) and Qi and blood deficiency syndrome (QBDS) groups. Gut microbial community profiles were analyzed by 16S rRNA gene sequencing using an Illumina MiSeq system. A retrospective study comparing hormone levels and gut microbiota information was performed between the SDBS and QBDS groups.

RESULTS: Compared with the QBDS group, the serum levels of estradiol (E2) and anti-Müllerian hormone (AMH) were significantly decreased in the SDBS group. The quantities of Adlercreutzia, Eggerthella, Klebsiella, and Paraprevotella significantly increased in the SDBS group, whereas Lactobacillus decreased significantly. Moreover, alterations in the microbiome in the SDBS and QBDS groups were closely related to the levels of E2 and AMH. The area under the receiver operating characteristic curve for the classification of the two syndromes by the gut microbiome was 0.71.

CONCLUSIONS: There were significant differences in the dominant microbiota between the SDBS and QBDS groups, and the change in Proteobacteria in the QBDS group was more significant. The characteristics of gut microbiota help us differentiate between the SDBS and QBDS groups, which may provide a basis for the objectification of TCM syndrome types.

Key words： primary ovarian insufficiency; gastrointestinal microbiome; RNA, ribosomal, 16S; gonadal steroid hormones; traditional Chinese syndromes

Cite this article

Jiaman WU , Yan NING , Liya TAN , Fei MA , Yanting LIN , Yuanyuan ZHUO . Difference of the gut microbiota of premature ovarian insufficiency in two traditional Chinese syndromes[J]. Journal of Traditional Chinese Medicine, 2025 , 45(1) : 132 -139 . DOI: 10.19852/j.cnki.jtcm.2025.01.012

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