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Journal of Traditional Chinese Medicine >

2025 , Vol. 45 >Issue 1: 1 - 12

DOI: https://doi.org/10.19852/j.cnki.jtcm.2025.01.001

Hewei Jiangni granule (和胃降逆颗粒) alleviates visceral hypersensitivity of non-erosive reflux disease via stromal interaction molecule 1/transient receptor potential vanilloid subfamily member 1 pathway

  • Yuan CHENG ,
  • Xiaosi ZHANG ,
  • Junxiang LI ,
  • Liming ZHANG ,
  • Yi DAI ,
  • Chune XIE ,
  • Lei SHI ,
  • Xiaohong LI ,
  • Fushun KOU
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  • 1 School of Traditional Chinese Medicine and School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China; Gastroenterology Department, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
    2 Gastroenterology Department, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
    3 Department of Pharmacotherapy and Oriental Medicine, School of Pharmacy, Hyogo University of Health Sciences, Hyogo 650-8530, Japan
    4 Center for IBD Research, Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China

Received date: 2023-06-11

  Accepted date: 2023-10-22

  Online published: 2025-01-10

Supported by

National Natural Science Foundation of China: Study on the Molecular Mechanism of the Regulation of Crypt Goblet Cell Pyroptosis and Exocytosis to Repair Ulcerative Colitis Mucus Barrier by the Method of Clearing and Opening the Xuanfu from the Perspective of "Xuanfu-Crypt"(82305143);National Natural Science Foundation of China: Exploring the Molecular Mechanism of "Hewei Jiangni Fang" Intervention in Non-erosive Reflux Disease Esophageal Hypersensitivity from the Perspective of Mas-related Gene X2/Stromal Interaction Molecule 1/Cell Adhesion Molecule 1 Pathway Regulation of Mast Cell/Dorsal Root Ganglion Communication based on the "Xinkai-Kujiang" Method(82374401)

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Abstract

OBJECTIVE: To explore if Hewei Jiangni granule (和胃降逆颗粒, HWJNG) could regulate esophageal hypersensitivity via stromal interaction molecule 1 (STIM1)/transient receptor potential vanilloid subfamily member 1 (TRPV1) pathway.

METHODS: Qualitative analysis of HWJNG was analysis by high performance of liquid and gas chromatography. In vivo, animal model of non-erosive reflux disease (NERD) was established by fructose intake and restraint stress. HWJNG and Omeprazole were administered by gavage to the drug intervention group. Reflux and visceral hypersensitivity were analyzed by pathological changes, PH value test, mechanical paw withdrawal threshold, thermal withdrawal latency and mast cells (MCs) degranulation. In vitro, substance P (SP)-induced P815 cells and dorsal root ganglion (DRG) cells were co-cultured. Expression in both mice and cells of STIM1, TRPV1, and esophageal visceral hypersensitivity-related gastrointestinal neurochemicals were validated by enzyme linked immunosorbent assays, quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Moreover, overexpression and small interfering RNA against STIM1 were utilized to verify of the role of HWJNG in DRG cells.

RESULTS: HWJNG significantly suppressed intercellular space widening, injury of mitochondrial, MCs degranulation, mechanical allodynia and heat neuropathic sensory and increased pH value of esophageal mucosa in NERD mice. HWJNG inhibited expression of visceral hypersensitivity-related gastrointestinal neurochemicals in esophageal mucosa and activated P815 cells, and expression of the STIM1, TRPV1 and related neurotransmitters in DRG and DRG cells. STIM1 siRNA and HWJNG both reduced P815 cells adhesion to DRGs cells and Ca2+ flow into the cytoplasmic space of DRG cells. Furthermore, HWJNG could reversed STIM1 overexpression induced upregulation of TRPV1.

CONCLUSION: HWJNG suppressed intercellular space widening in NERD mice, stabilized MCs and restored neuronal hyperexcitability by regulating visceral hypersensitivity viaSTIM1/TRPV1 pathway.

Key words： non-erosive reflux disease; visceral hypersensitivity; stromal interaction molecule 1; transient receptor potential channels; Hewei Jiangni granule

Cite this article

Yuan CHENG , Xiaosi ZHANG , Junxiang LI , Liming ZHANG , Yi DAI , Chune XIE , Lei SHI , Xiaohong LI , Fushun KOU . Hewei Jiangni granule (和胃降逆颗粒) alleviates visceral hypersensitivity of non-erosive reflux disease via stromal interaction molecule 1/transient receptor potential vanilloid subfamily member 1 pathway[J]. Journal of Traditional Chinese Medicine, 2025 , 45(1) : 1 -12 . DOI: 10.19852/j.cnki.jtcm.2025.01.001

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