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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 6: 1187 - 1193

DOI: https://doi.org/10.19852/j.cnki.jtcm.2024.06.007

Research Articles

Untargeted serum metabonomic reveals alleviated ovalbumin-induced asthma by Baijin Pingchuan (白金平喘) through primary bile acid biosynthesis

  • Lizhong DING ,
  • Qiang ZHANG ,
  • Yingying SUN ,
  • Yibu KONG ,
  • Yongfu SONG ,
  • Yongji WANG
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  • Department of Pediatrics, the Affiliated hospital to Changchun University of Chinese Medicine, Changchun 130017, China

Received date: 2023-07-22

  Accepted date: 2023-11-27

  Online published: 2024-11-12

Supported by

Science and Technology Development Plan Project of Jilin Provincial: Research on the Development of Baijin Pingchuan Granules as an In-hospital Preparation for the Treatment of Pediatric Asthma (Heat-type Asthma)(20200404043YY);Traditional Chinese Medicine Science and Technology Project of Jilin Provincial: Exploring the Mechanism of Baijin Pingchuan Granules in Alleviating Airway Inflammation in Asthma based on the “Three-Stage Differentiation and Treatment” Theory of National Medical Masters(2020090)

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Abstract

OBJECTIVE: To investigate the effect of baijinpingchuan (白金平喘, BJPC) on the asthma rat model and identify differential metabolites and disturbed metabolic pathways.

METHODS: The rats were categorized into six groups: control, dexamethasone (DEX), ovalbumin (OVA), and low-, median-, and high-dose BJPC. The rats, except for the control group, were initially treated with OVA to develop the asthma model, which was then activated using DEX, OVA, and low-, median-, and high-dose BJPC. Enzyme-linked immunosorbent assay kit was used to detect the expression of interleukin (IL)-33, IL-25, thymic stromal lymphopoietin (TSLP), and transforming growth factor-beta 1 (TGF-β1). Hematoxylin and eosin staining were performed to observe the pathological condition of the lung. Untargeted serum metabonomic analysis was conducted to identify differential metabolites and disturbed metabolic pathways.

RESULTS: High-dose BJPC significantly inhibited the expression of IL-33, IL-25, TSLP, and TGF-β1 (P < 0.0001). Further, high-dose BJPC improved inflammatory cell infiltration, which plays a similar role in asthma as DEX. OVA-induced and BJPC-treated rats were identified through 17 differential metabolites, especially cholic acid. Furthermore, primary bile acid biosynthesis was a significantly differential pathway in the mechanism of BJPC for treating asthma.

CONCLUSIONS: BJPC plays an anti-inflammation role in asthma, which might be a promising therapy through mediating primary bile acid biosynthesis.

Key words： asthma; ovalbumin; untargeted serum metabonomic; Baijin Pingchuan

Cite this article

Lizhong DING , Qiang ZHANG , Yingying SUN , Yibu KONG , Yongfu SONG , Yongji WANG . Untargeted serum metabonomic reveals alleviated ovalbumin-induced asthma by Baijin Pingchuan (白金平喘) through primary bile acid biosynthesis[J]. Journal of Traditional Chinese Medicine, 2024 , 44(6) : 1187 -1193 . DOI: 10.19852/j.cnki.jtcm.2024.06.007

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