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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 6: 1146 - 1152

DOI: https://doi.org/10.19852/j.cnki.jtcm.2024.06.005

Research Articles

Anti-oxidative and immunological role of Cyclocarya paliurus polysaccharide on the liver injury of diabetic rats

  • Xichao XIA ,
  • Shipeng XUE ,
  • Guoying SONG ,
  • Bin LI ,
  • Huiping WANG ,
  • Ju QIU ,
  • Jihong Wang ,
  • Qingchun LIU ,
  • Yuhong MA ,
  • Jingfeng OUYANG
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  • 1 Nursing College, Pingdingshan University, Pingdingshan 467000, China
    2 Basic Medicine College of Nanyang Medical University, Nanyang 473041, China
    3 Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China

Received date: 2023-03-10

  Accepted date: 2023-06-16

  Online published: 2024-11-12

Supported by

Effect of Cyclocarya Paliurus Polysaccharides on the Diabetes Mellitus(212102310845);Effect of Kelening decoction on diabetic rats(242102310556)

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Abstract

OBJECTIVE: To investigate the effects of Cyclocarya paliurus (C. paliurus) polysaccharide on the liver injury of diabetic rats.

METHODS: Rats were divided into 6 groups, including normal group, model group, control group, low-dose group of C. paliurus polysaccharide treatment, middle-dose group of C. paliurus polysaccharide treatment and high-dose group of C. paliurus polysaccharide treatment. Histological analysis of liver was analyzed using hematoxilin and eosin. Levels of plasma biological parameters and anti-oxidative enzymes were determined by spectrophotometry. Nuclear factor kappa-B-p65 (NF-κB p65), tumor necrosis factor-α (TNF-α), interleukins 6 (IL-6), IL-1β were measured by real-time polymerase chain reaction and enzyme-linked immunosorbent assay.

RESULTS: Compared with that of model group, the glucose level of plasma decreased 62.32% (P < 0.01), but glycogen and insulin level increased 1.51 times and 1.27 times in the high-dose group of C. paliurus polysaccharide treatment, respectively. Plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) level decreased 47.47% (P < 0.05), 43.65% (P < 0.05) and 50.51% (P < 0.05) in the high-dose group of C. paliurus polysaccharide treatment, respectively. Superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase level increased 1.16 times (P < 0.01), 71.28% (P < 0.05), 1.29 times (P < 0.01) and 87.46% (P < 0.05) in the high-dose group of C. paliurus polysaccharide treatment, respectively. Enzyme activities of NF-κB, TNF-α, IL-1β and IL-6 were decreased 39.29% (P < 0.05), 51.11% (P < 0.05), 37.42% (P < 0.05) and 36.50% (P < 0.05), respectively.

CONCLUSIONS: Administration of C. paliurus polysaccharide may play a protecting role for liver injury of diabetic rats through lowering glucose, ALT, AST, ALP level, increasing glycogen and insulin level, enhancing the anti-oxidative ability and down-regulating the inflammatory factors expression.

Key words： Cyclocarya paliurus polysaccharide; liver injury; diabetic rats; antioxidants; inflammatory factors

Cite this article

Xichao XIA , Shipeng XUE , Guoying SONG , Bin LI , Huiping WANG , Ju QIU , Jihong Wang , Qingchun LIU , Yuhong MA , Jingfeng OUYANG . Anti-oxidative and immunological role of Cyclocarya paliurus polysaccharide on the liver injury of diabetic rats[J]. Journal of Traditional Chinese Medicine, 2024 , 44(6) : 1146 -1152 . DOI: 10.19852/j.cnki.jtcm.2024.06.005

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