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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 5: 963 - 973

DOI: https://doi.org/10.19852/j.cnki.jtcm.2024.05.004

Role of toll-like receptor 4/mutant myeloid differentiation primary response 88/nuclear factor kappa-B mediated inflammation in diabetes mellitus with Northwest dryness syndrome

  • Deqiang DENG ,
  • Yan XIAO ,
  • Dan MA ,
  • Jinling QIU ,
  • Congli HAO ,
  • Di WANG ,
  • Miao ZHANG
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  • 1 State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi 830054, China
    2 Department of Endocrinology, Traditional Chinese Medicine Hospital of Urumqi, Urumqi 830099, China

Received date: 2023-06-15

  Accepted date: 2023-11-20

  Online published: 2024-09-11

Supported by

Correlation Study on Chronic Inflammation Mediated by Toll-like Receptor 4/Mutant Myeloid Differentiation Primary Response 88/Nuclear Factor Kappa-B Signaling Pathway(SKL-HIDCA-2021-ZY5)

Fold

Abstract

OBJECTIVE: To investigate the role of toll-like receptor 4 (TLR4)/mutant myeloid differentiation primary response 88 (MyD88)/nuclear factor kappa-B (NF-κB) signaling pathway-mediated inflammation in diabetes mellitus with Northwest dryness syndrome.

METHODS: Rats were randomly divided into the normal control, type 2 diabetes (T2DM) model, Northwest dryness syndrome + T2DM (Northwest dryness), and simple internal dampness + T2DM (internal dampness) groups. Enzyme-linked immunosorbent assay was used to detect biochemical indexes and inflammatory factors. The histopathological observation was performed. Quantitative real-time polymerase chain reaction and Western blot analysis were used to detect the mRNA and protein expression levels, respectively.

RESULTS: Compared with the T2DM group, the glycosylated hemoglobin A1c, insulin, glucose tolerance, the homeostasis model assessment of insulin resistance, tumor necrosis factor-α, interleukin 1β, interleukin 16, malondialdehyde, blood lipid, alanine aminotransferase, and aspartate aminotransferase were significantly elevated in the internal dampness group. Their levels were significantly elevated in the Northwest dryness group than in the T2DM and internal dampness groups. The superoxide dismutase, glutathione peroxidase, liver glycogen, and organ-to-weight ratio were significantly declined in the internal dampness group and the Northwest dryness group than in the T2DM group. However, these levels were elevated in the Northwest dryness group than in the internal dampness group. Moreover, the mRNA expression levels of interferon regulatory factor 5 and NF-κB p65, and the protein expression levels of TLR4, MyD88, and NF-κB were significantly higher in the internal dampness and the Northwest dryness groups than the T2DM group. Additionally, the mRNA and protein levels were significantly higher in the Northwest dryness group than in the internal dampness group.

CONCLUSION: Northwest dryness syndrome-mediated TLR4/MyD88/NF-κB pathway and chronic inflammation might be associated with the occurrence and development of T2DM.

Key words： toll-like receptor 4; myeloid differentiation factor 88; NF-kappa B; inflammation; diabetes mellitus; Northwest dryness syndrome

Cite this article

Deqiang DENG , Yan XIAO , Dan MA , Jinling QIU , Congli HAO , Di WANG , Miao ZHANG . Role of toll-like receptor 4/mutant myeloid differentiation primary response 88/nuclear factor kappa-B mediated inflammation in diabetes mellitus with Northwest dryness syndrome[J]. Journal of Traditional Chinese Medicine, 2024 , 44(5) : 963 -973 . DOI: 10.19852/j.cnki.jtcm.2024.05.004

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