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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 5: 954 - 962

DOI: https://doi.org/10.19852/j.cnki.jtcm.20240806.003

Qingfei Zhisou oral liquid (清肺止嗽口服液) alleviates fever-induced inflammation by regulating arachidonic acid and lysophospholipids metabolism and inhibiting hypothalamus transient receptor potential ion channels expression

  • Jiaming GAO ,
  • Yehao ZHANG ,
  • Yuanyuan Chen ,
  • Long JIN ,
  • Jianfeng ZHAO ,
  • Hao GUO ,
  • Jianhua FU
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  • 1 Institute of Basic Medical Sciences, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing 100091, China
    2 Pharmaceutical R&D Department, Taiyuan Yidavike Medical Technology Development Co., Ltd., Taiyuan 030006, China

Received date: 2023-05-06

  Accepted date: 2023-09-05

  Online published: 2024-08-06

Supported by

Beijing Traditional Chinese Medicine Foundation for Science and Technology(JJ-2020-78);Research on the Protection of Intellectual Property Rights of Traditional Chinese Medicine; Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(CI2021A00912);Basic and Translational Research on the Application of Traditional Chinese Medicine; the Research Foundation of Major New Drug Creation, from the Ministry of Science and Technology of China(2018ZX09721003-009-022);Research on Key Technologies and Industrialization of Traditional Chinese Medicine Preparations for Children;National Natural Science Foundation of China(82074060);Study on the Effect of Tanyu Tongzhi Fang in Maintaining Vascular Homeostasis in the Treatment of Atherosclerosis

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Abstract

OBJECTIVE: To explore how Qingfei Zhisou oral liquid (清肺止嗽口服液, QFZS) adjusts body temperature bias and the interaction of inflammatory factors levels and metabolomic differences.

METHODS: Dry yeast was subcutaneously injected at 10 mL/kg to establish the pyrexia model. We randomly divided 60 Sprague-Dawley rats into five groups: control, model, positive, low dose of QFZS and high dose of QFZS. Inflammatory proteins were evaluated by Western blotting and immunohistochemistry. For the examination of the endogenous metabolites, enzyme linked immunosorbent assay and ultra-high-performance liquid chromatography high-resolution mass spectrometry were employed.

RESULTS: QFZS significantly reduced rats' body temperature within 6 h after dry yeast injection and reduced the secretion of the arginine vasopressin, cyclic adenosine monophosphate, prostaglandin E-2, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β in serum. Meanwhile, we identified 41 metabolites between the model and QFZS groups, including arachidonic acid and lysophospholipids. QFZS restored normal arachidonic acid levels. Based on the differential metabolite enrichment analysis, QFZS's anti-inflammatory and anti-pyrexia effects might be related to the inflammatory pathway regulated by transient receptor potential. Additionally, QFZS treatment reduced transient receptor potential melastatin 2 ion channel expression and affected TNF-α, heat shock protein 70, and cyclooxygenase-2 expression in the hypothalamus.

CONCLUSION: QFZS exerts its regulatory effects on fever by regulating the metabolism of lysophospholipids and arachidonic acid and the regulation of inflammation via transient receptor potential ion channels channels.

Key words： inflammation; fever; metabolomics; transient receptor potential ion channels; Qingfei Zhisou oral liquid

Cite this article

Jiaming GAO , Yehao ZHANG , Yuanyuan Chen , Long JIN , Jianfeng ZHAO , Hao GUO , Jianhua FU . Qingfei Zhisou oral liquid (清肺止嗽口服液) alleviates fever-induced inflammation by regulating arachidonic acid and lysophospholipids metabolism and inhibiting hypothalamus transient receptor potential ion channels expression[J]. Journal of Traditional Chinese Medicine, 2024 , 44(5) : 954 -962 . DOI: 10.19852/j.cnki.jtcm.20240806.003

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