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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 5: 896 - 905

DOI: https://doi.org/10.19852/j.cnki.jtcm.20240806.001

Actinidia chinensis polysaccharide interferes with the epithelial-mesenchymal transition of gastric cancer by regulating the nuclear transcription factor-κB pathway to inhibit invasion and metastasis

  • Guangshun ZHANG ,
  • Xiaonan XU ,
  • Chuyun XU ,
  • Guanghui LIAO ,
  • Hao XU ,
  • Zhaohuan LOU ,
  • Guangji ZHANG
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  • 1 School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China
    2 Key Labortary of Blood-stasis-toxin Syndrome of Zhejiang Province, Hangzhou 310053, China
    3 School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China
    4 Key Labortary of Blood-stasis-toxin Syndrome of Zhejiang Province, Hangzhou 310053, China
    5 Traditional Chinese Medicine "Preventing Disease" Wisdom Health Project Research Center of Zhejiang, Hangzhou 310053, China
    6 the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310053, China

Received date: 2023-04-21

  Accepted date: 2023-08-29

  Online published: 2024-08-06

Supported by

Innovative Research and Application based on the Core Etiology and Pathogenesis of Different Diseases and the Same Treatment Plan based on "Blood Stasis, Toxin and Depression"(2019YFC1708700);Innovative Research and Application based on the Core Etiology and Pathogenesis of Different Diseases and the Same Treatment Plan based on "Blood Stasis, Toxin and Depression"(2019YFC1708701);Biological Basic Research on Gastric Cancer-Carcinoma Transformation and Intervention by Removing Blood Stasis and Detoxification Based on the Pathogenesis Theory of Blood Stasis and Toxin Interaction(82030119);Study on the Mechanism of Tanshinone Combined with Arsenic Trioxide Stasis and Poison to Regulate Macrophage Polarization and Improve the Inflammatory Microenvironment of Liver Cancer(81874455);Research on Innovative Traditional Chinese Medicine Drugs-preclinical Research on Anti-lung Cancer of Diterpenoid Tanshinone(2019C03072);Study on the Diagnosis and Treatment Program of Damp-heat Accumulation Type Colorectal Cancer Based on Correlation Analysis of Intestinal Microbiota- Traditional Chinese Medicine Syndrome Type(2020ZX005);Research on Quantitative Characterization of Preparation Process and Quality Standard Control Specification of Traditional Chinese Medicine Paste (Gao Zi Ji)(2019ZZ006);Study on the Molecular Mechanism of Actinidia Chinensis Polysaccharide Interfering with Epithelial Mesenchymal Transformation in Gastric Cancer by Inhibiting Nuclear Factor-κB Pathway(81273904);Study on the Mechanism of the Effective Components of Tengli Root Regulating Tumor-associated Fibroblasts to Improve the Tumor Microenvironment and Prevent the Metastasis of Gastric Cancer(2022JKZKTS16)

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Abstract

OBJECTIVE: To investigate the mechanisms of the effect of Actinidia chinensis polysaccharide (ACPS) on the invasion and metastasis of gastric cancer cells.

METHODS: BGC-823-Luc gastric cancer cells stably transfected with a luciferase gene were used to establish an insitutransplanted tumor mouse model. A live mouse imaging system was used to observe tumor growth, and hematoxylin and eosin staining was applied to analyze tissue histopathology. Transwell and scratch wound assays were performed to examine the invasive and migratory ability of BGC-823 cells. Immunofluorescence, confocal microscopy, immunohistochemistry, and Western blot assays were used to analyze the expressions of the nuclear transcription factor-κB (NF-κB) signaling pathway and epithelial-mesenchymal transition (EMT)-related proteins.

RESULTS: ACPS significantly inhibited the growth of subcutaneously transplanted BGC-823-Luc gastric cancer tumors in nude mice and reduced inflammatory cell infiltration in tumor tissues. ACPS inhibited Epidermal Growth Factor-induced invasion, migration, and morphological changes in the cytoskeleton of BGC-823 cells. ACPS inhibited gastric cancer EMT and decreased the expression of matrix metallopeptidase 9, N-cadherin and p-NF-κB p65 in transplanted tumor tissues. ACPS inhibited the expression of matrix metalloproteinases and vascular adhesion factors in BGC-823 cells, promoted p65-NF-κB nuclear translocation, and regulated proteins associated with the NF-κB p65 pathway.

CONCLUSIONS: ACPS inhibited gastric cancer invasion and metastasis both in vivo and in vitro, which evidenced the inhibition of gastric cancer EMT viaregulating the NF-κB inflammatory pathway.

Key words： stomach neoplasms; invasion; neoplasm metastasis; epithelial-mesenchymal transition; NF-kappa B; signal transduction; Actinidia chinensis polysaccharide

Cite this article

Guangshun ZHANG , Xiaonan XU , Chuyun XU , Guanghui LIAO , Hao XU , Zhaohuan LOU , Guangji ZHANG . Actinidia chinensis polysaccharide interferes with the epithelial-mesenchymal transition of gastric cancer by regulating the nuclear transcription factor-κB pathway to inhibit invasion and metastasis[J]. Journal of Traditional Chinese Medicine, 2024 , 44(5) : 896 -905 . DOI: 10.19852/j.cnki.jtcm.20240806.001

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