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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 5: 1024 - 1034

DOI: https://doi.org/10.19852/j.cnki.jtcm.2024.05.009

Association of miR-499 rs3746444, miR-149 rs2292832 polymorphisms and their expression levels with helicobacter pylori-related gastric diseases and Traditional Chinese Medicine syndromes

  • Qi LIU ,
  • Chang YU ,
  • Jintong YE ,
  • Ling ZHANG ,
  • Danyan LI ,
  • Yunkai DAI ,
  • Yunzhan ZHANG ,
  • Qi LUO ,
  • Weijing CHEN ,
  • Huaigeng PAN ,
  • Ruliu LI ,
  • Ling HU
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  • 1 Institute of Gastroenterology, Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou 510000, China
    2 First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510000, China

Received date: 2023-06-22

  Accepted date: 2023-09-15

  Online published: 2024-09-11

Supported by

Exploration of the Traditional Chinese Medicine Syndrome Characteristics During Benign and Malignant Pathological Evolution in Hp-Related Gastric Diseases from Wnt/β-catenin Signaling Pathway based on the Theory of "Evil Poison Lead to Change"(81774238);Exploration of the Traditional Chinese Medicine Syndrome Characteristics During Benign and Malignant Pathological Evolution in Hp-Related Gastric Diseases based on the Theory of "Latent Toxin" and the Polymorphism and Expression of Mucosal Barrier Related Genes(82174298)

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Abstract

OBJECTIVE: To provide an objective experimental basis for the gastric mucosa pathological evolution and the transformation of different Traditional Chinese Medicine (TCM) syndromes in helicobacter pylori (H. pylori)-related gastric diseases (HPGD) patients, based on the combination of TCM syndrome differentiation, molecular biology and histopathology.

METHODS: A total of 203 participants were enrolled in this study. The expressions of miR-499/miR-149 and H. pylori infection in the gastric tissues from all participants were detected. The genotyping for miR-499 rs3746444 and miR-149 rs2292832 was performed.

RESULTS: In H. pylori positive subjects, the proportion of precancerous gastric lesions (PGL) in liver-stomach disharmony syndrome (LSDS) group was higher than in spleen Qi deficiency syndrome (SQDS) group (P <0.001); The proportion of gastric cancer (GC) in SQDS group was higher than in spleen-stomach damp-heat syndrome (SSDHS) group and LSDS group (all P <0.001). We also found C allele of miR-149 rs2292832 was linked to lower risk of gastric atrophy [miR-149 rs2292832 C vs T: adjusted odds ratio = 0.207; 95% confidence interval (0.043-0.989); P = 0.048]. Compared with healthy control (HC) group, the expression of miR-499 was significantly increased in GC group, while the expression of miR-149 was significantly decreased in chronic inflammation group, PGL group and GC group (all P < 0.05). Test for trend showed that GC risk was on a rising trend with the increasing expression of miR-499 and decreasing expression of miR-149 (both P for trend < 0.05).

CONCLUSION: The C allele of miR-149 rs2292832 may be a protective factor for gastric mucosal atrophy. H. pylori may participate in the evolution of benign to malignant gastric mucosa lesions by inducing the overexpression of miR-499 and down regulation of miR-149. In addition, patients with H. pylori infection combined SQDS or LSDS may have higher risk of gastric mucosal malignant lesions.

Key words： microRNAs; helicobacter pylori; stomach diseases; polymorphism, genetic; syndrome complex

Cite this article

Qi LIU , Chang YU , Jintong YE , Ling ZHANG , Danyan LI , Yunkai DAI , Yunzhan ZHANG , Qi LUO , Weijing CHEN , Huaigeng PAN , Ruliu LI , Ling HU . Association of miR-499 rs3746444, miR-149 rs2292832 polymorphisms and their expression levels with helicobacter pylori-related gastric diseases and Traditional Chinese Medicine syndromes[J]. Journal of Traditional Chinese Medicine, 2024 , 44(5) : 1024 -1034 . DOI: 10.19852/j.cnki.jtcm.2024.05.009

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