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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 5: 1017 - 1023

DOI: https://doi.org/10.19852/j.cnki.jtcm.20240828.007

Relative exchangeable copper, a high-quality biomarker for differentiation of Traditional Chinese Medicine syndrome in Wilson’s disease

  • Yufeng DING ,
  • Wenming YANG ,
  • Yuan CHENG ,
  • Yulong YANG ,
  • Jun ZHU ,
  • Yachun LU ,
  • Xiang FANG ,
  • Jing ZHANG
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  • 1 Department of Neurology, the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei 230031, China
    2 Department of clinical laboratory, the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei 230031, China

Received date: 2023-06-11

  Accepted date: 2023-11-27

  Online published: 2024-09-11

Supported by

Research on the Neuroprotective Mechanism of the Gandouling based on the Regulation of Long Non-coding Ribonucleic Acid Maternally Expressed Gene 3 and Targets Gene Forkhead Box O1 through the Way of Autophagy in Wilson Disease(82104783);Study on Mechanism of Xin’an Medical Gubenpeiyuan Prescription Treating Wilson’s Disease based on Enterohepatic Axis and Enterobrain Axis Pathway(U22A20366);2019 Project of Building Evidence based Practice Capacity for Traditional Chinese Medicine(2019XZZX-NB001);Mechanism Study on the Regulation of Peroxisome Proliferator-activated Receptor-gamma Coactivator-1alpha/Sirtuin-3/Mitochondrial Axis to Maintain Hippocampal Synaptic Homeostasis to Improve Cognitive Impairment in Wilson’s Disease by Qingre Lidan JieDu Prescription(2008085MH264);Mitofusin 2 Mediates the Role of Endoplasmic Reticulum-MAMs-mitochondria Regulation of Synaptic Homeostasis in Wilson’s Disease Neurons and the Mechanism of Qingre Lidan Jiedu Prescription Intervention(2208085MH271)

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Abstract

OBJECTIVE: To investigate the value of relative exchangeable copper (REC) in diagnosing Wilson’s disease (WD) and to determine its significance in the differentiation of Traditional Chinese Medicine (TCM) syndrome.

METHODS: A total of 78 patients with WD were recruited on the same day of the medical visit, and among them, 32 were suffering from non-WD (N-WD) and 37 were heterozygous ATP7B carriers (HC) enrolled as controls. Molecular genetic testing was performed for diagnosing WD and HC. Additionally, REC levels in different TCM syndromes were investigated. The correlation between REC and serum ceruloplasmin (Cp), serum copper oxidase (CO), and 24-h urinary copper was analyzed using the Global Assessment Scale and Unified Wilson’s Disease Rating Scale and the significance of REC in WD diagnosis was investigated. Resting-state functional magnetic resonance imaging was used to assess the clinical symptoms of WD and analyze its severity in different TCM syndromes.

RESULTS: REC determination helped in significantly distinguishing patients with N-WD or HC from those with WD with a cut-off of 21.15%. Furthermore, the comparative analysis of REC ratios among different TCM syndromes showed markedly high REC levels in the dampness-heat internal accumulation syndrome group. Additionally, the seed-based functional connectivity value from the putamen to the cerebellum was significantly correlated with TCM syndromes.

CONCLUSION: REC level is a high-quality biomarker for diagnosing WD that effectively reflects disease severity and plays an essential role in deciding on treatment strategies and prognosis. Furthermore, REC levels are closely related to TCM syndromes in WD; thus, it is a potential objective quantitative indicator for distinguishing TCM syndromes in WD.

Key words： Wilson’s disease; relative exchangeable copper; Traditional Chinese Medicine syndrome; Global Assessment Scale; the Unified Wilson’s Disease Rating Scale

Cite this article

Yufeng DING , Wenming YANG , Yuan CHENG , Yulong YANG , Jun ZHU , Yachun LU , Xiang FANG , Jing ZHANG . Relative exchangeable copper, a high-quality biomarker for differentiation of Traditional Chinese Medicine syndrome in Wilson’s disease[J]. Journal of Traditional Chinese Medicine, 2024 , 44(5) : 1017 -1023 . DOI: 10.19852/j.cnki.jtcm.20240828.007

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